Restriction map for
pF1KE0525
(Linear) (Six-base) MAPSORT of: KIEE0525.seq Check: 6057 from: 1 to: 1152
KIEE0525.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 56 856 1035 1152
Size: 56 800 179 117
Fragments arranged by size:
800 179 117 56
AleI CACnn'nnGTG
Cuts at: 0 1141 1152
Size: 1141 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 185 1152
Size: 185 967
AlwNI CAG_nnn'CTG
Cuts at: 0 449 459 964 1152
Size: 449 10 505 188
Fragments arranged by size:
505 449 188 10
ApaI G_GGCC'C
Cuts at: 0 437 1152
Size: 437 715
ApaLI G'TGCA_C
Cuts at: 0 214 1152
Size: 214 938
ApoI r'AATT_y
Cuts at: 0 409 1152
Size: 409 743
AvaI C'yCGr_G
Cuts at: 0 897 945 1152
Size: 897 48 207
Fragments arranged by size:
897 207 48
BanI G'GyrC_C
Cuts at: 0 707 868 1152
Size: 707 161 284
Fragments arranged by size:
707 284 161
BanII G_rGCy'C
Cuts at: 0 437 1106 1152
Size: 437 669 46
Fragments arranged by size:
669 437 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 1152
Size: 155 997
BglI GCCn_nnn'nGGC
Cuts at: 0 265 1152
Size: 265 887
BlpI GC'TnA_GC
Cuts at: 0 1019 1152
Size: 1019 133
Bme1580I G_kGCm'C
Cuts at: 0 218 437 871 1152
Size: 218 219 434 281
Fragments arranged by size:
434 281 219 218
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 912 1152
Size: 912 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 126 1152
Size: 46 80 1026
Fragments arranged by size:
1026 80 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 282 1152
Size: 282 870
BsaAI yAC'GTr
Cuts at: 0 219 1152
Size: 219 933
BsaWI w'CCGG_w
Cuts at: 0 559 1152
Size: 559 593
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 1152
Size: 202 950
BsiHKAI G_wGCw'C
Cuts at: 0 218 786 878 1106 1152
Size: 218 568 92 228 46
Fragments arranged by size:
568 228 218 92 46
BsmI GAATG_Cn'
Cuts at: 0 37 1152
Size: 37 1115
BsmBI CGTCTCn'nnnn_
Cuts at: 0 192 1152
Size: 192 960
Bsp1286I G_dGCh'C
Cuts at: 0 218 437 786 871 878 1106 1152
Size: 218 219 349 85 7 228 46
Fragments arranged by size:
349 228 219 218 85 46 7
BspEI T'CCGG_A
Cuts at: 0 559 1152
Size: 559 593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 856 1035 1070 1152
Size: 56 800 179 35 82
Fragments arranged by size:
800 179 82 56 35
BsrBI CCG'CTC
Cuts at: 0 54 1152
Size: 54 1098
BsrDI GCAATG_nn'
Cuts at: 0 329 827 1152
Size: 329 498 325
Fragments arranged by size:
498 329 325
BsrGI T'GTAC_A
Cuts at: 0 731 1152
Size: 731 421
BstAPI GCAn_nnn'nTGC
Cuts at: 0 829 1152
Size: 829 323
BstBI TT'CG_AA
Cuts at: 0 131 1152
Size: 131 1021
BstXI CCAn_nnnn'nTGG
Cuts at: 0 928 1152
Size: 928 224
BtgI C'CryG_G
Cuts at: 0 542 1152
Size: 542 610
BtsI GCAGTG_nn'
Cuts at: 0 837 1152
Size: 837 315
DraIII CAC_nnn'GTG
Cuts at: 0 657 1152
Size: 657 495
EaeI y'GGCC_r
Cuts at: 0 770 1152
Size: 770 382
EcoICRI GAG'CTC
Cuts at: 0 1104 1152
Size: 1104 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 126 1152
Size: 46 80 1026
Fragments arranged by size:
1026 80 46
HaeII r_GCGC'y
Cuts at: 0 11 1152
Size: 11 1141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 323 355 1152
Size: 4 32 287 32 797
Fragments arranged by size:
797 287 32 32 4
HincII GTy'rAC
Cuts at: 0 336 623 1152
Size: 336 287 529
Fragments arranged by size:
529 336 287
HindIII A'AGCT_T
Cuts at: 0 106 125 394 1152
Size: 106 19 269 758
Fragments arranged by size:
758 269 106 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1152
Size: 1148 4
MslI CAynn'nnrTG
Cuts at: 0 481 521 1141 1152
Size: 481 40 620 11
Fragments arranged by size:
620 481 40 11
MspA1I CmG'CkG
Cuts at: 0 184 195 1152
Size: 184 11 957
Fragments arranged by size:
957 184 11
PasI CC'CwG_GG
Cuts at: 0 119 1152
Size: 119 1033
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 1152
Size: 477 675
PfoI T'CCnGG_A
Cuts at: 0 350 497 1152
Size: 350 147 655
Fragments arranged by size:
655 350 147
PmlI CAC'GTG
Cuts at: 0 219 1152
Size: 219 933
PspOMI G'GGCC_C
Cuts at: 0 433 1152
Size: 433 719
PspXI vC'TCGA_Gb
Cuts at: 0 945 1152
Size: 945 207
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 682 714 1152
Size: 682 32 438
Fragments arranged by size:
682 438 32
PstI C_TGCA'G
Cuts at: 0 164 1152
Size: 164 988
PvuII CAG'CTG
Cuts at: 0 195 1152
Size: 195 957
SacI G_AGCT'C
Cuts at: 0 1106 1152
Size: 1106 46
SfcI C'TryA_G
Cuts at: 0 160 1128 1152
Size: 160 968 24
Fragments arranged by size:
968 160 24
SmlI C'TyrA_G
Cuts at: 0 945 1152
Size: 945 207
StyI C'CwwG_G
Cuts at: 0 400 1152
Size: 400 752
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 1152
Size: 595 557
TatI w'GTAC_w
Cuts at: 0 731 1152
Size: 731 421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 514 546 1152
Size: 514 32 606
Fragments arranged by size:
606 514 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 1152
Size: 861 291
XhoI C'TCGA_G
Cuts at: 0 945 1152
Size: 945 207
Enzymes that do cut:
AarI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbsI BglI BlpI Bme1580I BplI BpmI
BsaI BsaAI BsaWI BseRI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstXI
BtgI BtsI DraIII EaeI EcoICRI Eco57MI HaeII Hin4I
HincII HindIII MmeI MslI MspA1I PasI PflMI PfoI
PmlI PspOMI PspXI PsrI PstI PvuII SacI SfcI
SmlI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaHI BsaXI
BseYI BsgI BsiEI BsiWI BspHI BsrFI BssHII BssSI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EarI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PpiI
PpuMI PshAI PsiI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XmaI XmnI ZraI