Restriction map for
pF1KE0528
(Linear) (Six-base) MAPSORT of: KIEE0528.seq Check: 6691 from: 1 to: 864
KIEE0528.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 0 230 864
Size: 230 634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 864
Size: 70 794
AfeI AGC'GCT
Cuts at: 0 337 864
Size: 337 527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 745 755 864
Size: 745 10 109
Fragments arranged by size:
745 109 10
AlwNI CAG_nnn'CTG
Cuts at: 0 276 657 844 864
Size: 276 381 187 20
Fragments arranged by size:
381 276 187 20
ApaI G_GGCC'C
Cuts at: 0 296 864
Size: 296 568
ApaLI G'TGCA_C
Cuts at: 0 70 864
Size: 70 794
AvaI C'yCGr_G
Cuts at: 0 128 376 864
Size: 128 248 488
Fragments arranged by size:
488 248 128
BanI G'GyrC_C
Cuts at: 0 696 864
Size: 696 168
BanII G_rGCy'C
Cuts at: 0 214 296 377 688 864
Size: 214 82 81 311 176
Fragments arranged by size:
311 214 176 82 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 210 864
Size: 210 654
BglI GCCn_nnn'nGGC
Cuts at: 0 300 864
Size: 300 564
Bme1580I G_kGCm'C
Cuts at: 0 74 296 699 864
Size: 74 222 403 165
Fragments arranged by size:
403 222 165 74
BmrI ACTGGGnnnn_n'
Cuts at: 0 208 864
Size: 208 656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 286 586 864
Size: 202 84 300 278
Fragments arranged by size:
300 278 202 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 769 799 864
Size: 769 30 65
Fragments arranged by size:
769 65 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 202 658 864
Size: 199 3 456 206
Fragments arranged by size:
456 206 199 3
BseYI C'CCAG_C
Cuts at: 0 472 864
Size: 472 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 864
Size: 232 632
BsiHKAI G_wGCw'C
Cuts at: 0 74 440 864
Size: 74 366 424
Fragments arranged by size:
424 366 74
BsmI GAATG_Cn'
Cuts at: 0 93 864
Size: 93 771
BsmBI CGTCTCn'nnnn_
Cuts at: 0 125 864
Size: 125 739
Bsp1286I G_dGCh'C
Cuts at: 0 74 214 296 377 440 688 699 864
Size: 74 140 82 81 63 248 11 165
Fragments arranged by size:
248 165 140 82 81 74 63 11
BspHI T'CATG_A
Cuts at: 0 98 864
Size: 98 766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 476 864
Size: 476 388
BsrBI CCG'CTC
Cuts at: 0 548 785 864
Size: 548 237 79
Fragments arranged by size:
548 237 79
BsrDI GCAATG_nn'
Cuts at: 0 512 864
Size: 512 352
BssSI C'ACGA_G
Cuts at: 0 355 864
Size: 355 509
BstAPI GCAn_nnn'nTGC
Cuts at: 0 88 657 864
Size: 88 569 207
Fragments arranged by size:
569 207 88
BstEII G'GTnAC_C
Cuts at: 0 123 415 864
Size: 123 292 449
Fragments arranged by size:
449 292 123
BtgI C'CryG_G
Cuts at: 0 746 864
Size: 746 118
DraIII CAC_nnn'GTG
Cuts at: 0 360 864
Size: 360 504
EarI CTCTTCn'nnn_
Cuts at: 0 236 596 864
Size: 236 360 268
Fragments arranged by size:
360 268 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 202 286 586 864
Size: 70 132 84 300 278
Fragments arranged by size:
300 278 132 84 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 184 268 864
Size: 52 132 84 596
Fragments arranged by size:
596 132 84 52
EcoO109I rG'GnC_Cy
Cuts at: 0 45 273 293 486 810 864
Size: 45 228 20 193 324 54
Fragments arranged by size:
324 228 193 54 45 20
HaeII r_GCGC'y
Cuts at: 0 339 864
Size: 339 525
MslI CAynn'nnrTG
Cuts at: 0 170 172 864
Size: 170 2 692
Fragments arranged by size:
692 170 2
MspA1I CmG'CkG
Cuts at: 0 299 714 864
Size: 299 415 150
Fragments arranged by size:
415 299 150
NspI r_CATG'y
Cuts at: 0 27 864
Size: 27 837
PflMI CCAn_nnn'nTGG
Cuts at: 0 360 864
Size: 360 504
PpuMI rG'GwC_Cy
Cuts at: 0 273 810 864
Size: 273 537 54
Fragments arranged by size:
537 273 54
PspOMI G'GGCC_C
Cuts at: 0 292 864
Size: 292 572
SapI GCTCTTCn'nnn_
Cuts at: 0 236 864
Size: 236 628
SfcI C'TryA_G
Cuts at: 0 845 864
Size: 845 19
SphI G_CATG'C
Cuts at: 0 27 864
Size: 27 837
StyI C'CwwG_G
Cuts at: 0 62 674 864
Size: 62 612 190
Fragments arranged by size:
612 190 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 403 864
Size: 91 312 461
Fragments arranged by size:
461 312 91
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BglI Bme1580I BmrI BpmI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BssSI BstAPI BstEII BtgI DraIII
EarI Eco57MI EcoNI EcoO109I HaeII MslI MspA1I NspI
PflMI PpuMI PspOMI SapI SfcI SphI StyI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BsrFI BsrGI
BssHII BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI