Restriction map for
pF1KE0529
(Linear) (Six-base) MAPSORT of: KIEE0529.seq Check: 8712 from: 1 to: 642
KIEE0529.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
Acc65I G'GTAC_C
Cuts at: 0 140 506 642
Size: 140 366 136
Fragments arranged by size:
366 140 136
AflII C'TTAA_G
Cuts at: 0 607 642
Size: 607 35
AflIII A'CryG_T
Cuts at: 0 5 642
Size: 5 637
AlwNI CAG_nnn'CTG
Cuts at: 0 114 642
Size: 114 528
ApoI r'AATT_y
Cuts at: 0 241 519 642
Size: 241 278 123
Fragments arranged by size:
278 241 123
BanI G'GyrC_C
Cuts at: 0 140 506 642
Size: 140 366 136
Fragments arranged by size:
366 140 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 120 619 642
Size: 120 499 23
Fragments arranged by size:
499 120 23
BclI T'GATC_A
Cuts at: 0 410 642
Size: 410 232
BglII A'GATC_T
Cuts at: 0 65 642
Size: 65 577
Bme1580I G_kGCm'C
Cuts at: 0 480 642
Size: 480 162
BmrI ACTGGGnnnn_n'
Cuts at: 0 295 642
Size: 295 347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 304 367 443 569 642
Size: 90 214 63 76 126 73
Fragments arranged by size:
214 126 90 76 73 63
Bpu10I CC'TnA_GC
Cuts at: 0 28 642
Size: 28 614
BsaI GGTCTCn'nnnn_
Cuts at: 0 427 642
Size: 427 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 151 181 642
Size: 151 30 461
Fragments arranged by size:
461 151 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 642
Size: 193 449
BseYI C'CCAG_C
Cuts at: 0 57 398 642
Size: 57 341 244
Fragments arranged by size:
341 244 57
Bsp1286I G_dGCh'C
Cuts at: 0 480 642
Size: 480 162
BspHI T'CATG_A
Cuts at: 0 407 642
Size: 407 235
BsrGI T'GTAC_A
Cuts at: 0 224 642
Size: 224 418
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 642
Size: 144 498
BstYI r'GATC_y
Cuts at: 0 65 110 350 642
Size: 65 45 240 292
Fragments arranged by size:
292 240 65 45
EaeI y'GGCC_r
Cuts at: 0 41 642
Size: 41 601
EarI CTCTTCn'nnn_
Cuts at: 0 97 409 642
Size: 97 312 233
Fragments arranged by size:
312 233 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 268 642
Size: 268 374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 304 367 443 569 642
Size: 90 214 63 76 126 73
Fragments arranged by size:
214 126 90 76 73 63
KpnI G_GTAC'C
Cuts at: 0 144 510 642
Size: 144 366 132
Fragments arranged by size:
366 144 132
MscI TGG'CCA
Cuts at: 0 43 642
Size: 43 599
MslI CAynn'nnrTG
Cuts at: 0 184 642
Size: 184 458
PflMI CCAn_nnn'nTGG
Cuts at: 0 39 307 642
Size: 39 268 335
Fragments arranged by size:
335 268 39
PshAI GACnn'nnGTC
Cuts at: 0 601 642
Size: 601 41
SapI GCTCTTCn'nnn_
Cuts at: 0 97 409 642
Size: 97 312 233
Fragments arranged by size:
312 233 97
SexAI A'CCwGG_T
Cuts at: 0 136 642
Size: 136 506
SfcI C'TryA_G
Cuts at: 0 330 642
Size: 330 312
SmlI C'TyrA_G
Cuts at: 0 607 642
Size: 607 35
TatI w'GTAC_w
Cuts at: 0 224 555 642
Size: 224 331 87
Fragments arranged by size:
331 224 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 642
Size: 141 501
XmnI GAAnn'nnTTC
Cuts at: 0 7 642
Size: 7 635
Enzymes that do cut:
Acc65I AflII AflIII AlwNI ApoI BanI BbsI BclI
BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaXI BseRI
BseYI Bsp1286I BspHI BsrGI BstXI BstYI EaeI EarI
EciI Eco57MI KpnI MscI MslI PflMI PshAI SapI
SexAI SfcI SmlI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI ZraI