Restriction map for
pF1KE0531
(Linear) (Six-base) MAPSORT of: KIEE0531.seq Check: 8444 from: 1 to: 1233
KIEE0531.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 385 1233
Size: 385 848
AatII G_ACGT'C
Cuts at: 0 1112 1233
Size: 1112 121
AccI GT'mk_AC
Cuts at: 0 449 1112 1233
Size: 449 663 121
Fragments arranged by size:
663 449 121
Acc65I G'GTAC_C
Cuts at: 0 503 1193 1233
Size: 503 690 40
Fragments arranged by size:
690 503 40
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 361 1005 1160 1233
Size: 28 333 644 155 73
Fragments arranged by size:
644 333 155 73 28
AfeI AGC'GCT
Cuts at: 0 289 1233
Size: 289 944
AflIII A'CryG_T
Cuts at: 0 30 1233
Size: 30 1203
AlwNI CAG_nnn'CTG
Cuts at: 0 45 1233
Size: 45 1188
ApaI G_GGCC'C
Cuts at: 0 498 804 1233
Size: 498 306 429
Fragments arranged by size:
498 429 306
AvaI C'yCGr_G
Cuts at: 0 484 797 1233
Size: 484 313 436
Fragments arranged by size:
484 436 313
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 843 876 1233
Size: 843 33 357
Fragments arranged by size:
843 357 33
BamHI G'GATC_C
Cuts at: 0 279 921 1233
Size: 279 642 312
Fragments arranged by size:
642 312 279
BanI G'GyrC_C
Cuts at: 0 394 503 1193 1233
Size: 394 109 690 40
Fragments arranged by size:
690 394 109 40
BanII G_rGCy'C
Cuts at: 0 65 498 804 1233
Size: 65 433 306 429
Fragments arranged by size:
433 429 306 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 16 1008 1233
Size: 16 992 225
Fragments arranged by size:
992 225 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 1211 1233
Size: 1211 22
BclI T'GATC_A
Cuts at: 0 905 1163 1233
Size: 905 258 70
Fragments arranged by size:
905 258 70
BglI GCCn_nnn'nGGC
Cuts at: 0 808 1233
Size: 808 425
Bme1580I G_kGCm'C
Cuts at: 0 498 740 804 1233
Size: 498 242 64 429
Fragments arranged by size:
498 429 242 64
BmtI G_CTAG'C
Cuts at: 0 1007 1233
Size: 1007 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1233
Size: 442 791
BsaI GGTCTCn'nnnn_
Cuts at: 0 784 1233
Size: 784 449
BsaBI GATnn'nnATC
Cuts at: 0 910 1233
Size: 910 323
BsaHI Gr'CG_yC
Cuts at: 0 1109 1233
Size: 1109 124
BsaWI w'CCGG_w
Cuts at: 0 179 1233
Size: 179 1054
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 1042 1072 1233
Size: 313 30 699 30 161
Fragments arranged by size:
699 313 161 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 1047 1233
Size: 502 545 186
Fragments arranged by size:
545 502 186
BseYI C'CCAG_C
Cuts at: 0 24 1226 1233
Size: 24 1202 7
Fragments arranged by size:
1202 24 7
BsiHKAI G_wGCw'C
Cuts at: 0 20 677 704 1233
Size: 20 657 27 529
Fragments arranged by size:
657 529 27 20
BsmI GAATG_Cn'
Cuts at: 0 302 534 1233
Size: 302 232 699
Fragments arranged by size:
699 302 232
Bsp1286I G_dGCh'C
Cuts at: 0 20 65 498 677 704 740 804 1233
Size: 20 45 433 179 27 36 64 429
Fragments arranged by size:
433 429 179 64 45 36 27 20
BspHI T'CATG_A
Cuts at: 0 902 1233
Size: 902 331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 385 863 1233
Size: 385 478 370
Fragments arranged by size:
478 385 370
BsrBI CCG'CTC
Cuts at: 0 492 1233
Size: 492 741
BsrFI r'CCGG_y
Cuts at: 0 727 1233
Size: 727 506
BsrGI T'GTAC_A
Cuts at: 0 977 1233
Size: 977 256
BstEII G'GTnAC_C
Cuts at: 0 385 1233
Size: 385 848
BstYI r'GATC_y
Cuts at: 0 279 921 1233
Size: 279 642 312
Fragments arranged by size:
642 312 279
Bsu36I CC'TnA_GG
Cuts at: 0 927 1233
Size: 927 306
BtgI C'CryG_G
Cuts at: 0 80 1233
Size: 80 1153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 269 531 1233
Size: 269 262 702
Fragments arranged by size:
702 269 262
DrdI GACnn_nn'nnGTC
Cuts at: 0 412 1233
Size: 412 821
EaeI y'GGCC_r
Cuts at: 0 264 565 725 1065 1233
Size: 264 301 160 340 168
Fragments arranged by size:
340 301 264 168 160
EarI CTCTTCn'nnn_
Cuts at: 0 166 1233
Size: 166 1067
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 631 858 1233
Size: 631 227 375
Fragments arranged by size:
631 375 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 361 1005 1160 1233
Size: 28 333 644 155 73
Fragments arranged by size:
644 333 155 73 28
EcoO109I rG'GnC_Cy
Cuts at: 0 132 1233
Size: 132 1101
FseI GG_CCGG'CC
Cuts at: 0 731 1233
Size: 731 502
HaeII r_GCGC'y
Cuts at: 0 291 1010 1233
Size: 291 719 223
Fragments arranged by size:
719 291 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 264 266 296 1099 1131 1233
Size: 234 30 2 30 803 32 102
Fragments arranged by size:
803 234 102 32 30 30 2
KpnI G_GTAC'C
Cuts at: 0 507 1197 1233
Size: 507 690 36
Fragments arranged by size:
690 507 36
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1233
Size: 1019 214
MscI TGG'CCA
Cuts at: 0 567 1067 1233
Size: 567 500 166
Fragments arranged by size:
567 500 166
MslI CAynn'nnrTG
Cuts at: 0 169 379 743 973 1233
Size: 169 210 364 230 260
Fragments arranged by size:
364 260 230 210 169
MspA1I CmG'CkG
Cuts at: 0 110 563 1025 1071 1233
Size: 110 453 462 46 162
Fragments arranged by size:
462 453 162 110 46
NaeI GCC'GGC
Cuts at: 0 729 1233
Size: 729 504
NcoI C'CATG_G
Cuts at: 0 80 1233
Size: 80 1153
NgoMIV G'CCGG_C
Cuts at: 0 727 1233
Size: 727 506
NheI G'CTAG_C
Cuts at: 0 1003 1233
Size: 1003 230
NruI TCG'CGA
Cuts at: 0 254 1233
Size: 254 979
NspI r_CATG'y
Cuts at: 0 34 456 759 984 1233
Size: 34 422 303 225 249
Fragments arranged by size:
422 303 249 225 34
PasI CC'CwG_GG
Cuts at: 0 333 498 733 1233
Size: 333 165 235 500
Fragments arranged by size:
500 333 235 165
PciI A'CATG_T
Cuts at: 0 30 1233
Size: 30 1203
PflMI CCAn_nnn'nTGG
Cuts at: 0 86 879 1233
Size: 86 793 354
Fragments arranged by size:
793 354 86
PfoI T'CCnGG_A
Cuts at: 0 416 653 1233
Size: 416 237 580
Fragments arranged by size:
580 416 237
PpuMI rG'GwC_Cy
Cuts at: 0 132 1233
Size: 132 1101
PspOMI G'GGCC_C
Cuts at: 0 494 800 1233
Size: 494 306 433
Fragments arranged by size:
494 433 306
PstI C_TGCA'G
Cuts at: 0 71 562 719 893 1030 1233
Size: 71 491 157 174 137 203
Fragments arranged by size:
491 203 174 157 137 71
PvuII CAG'CTG
Cuts at: 0 563 1025 1071 1233
Size: 563 462 46 162
Fragments arranged by size:
563 462 162 46
SapI GCTCTTCn'nnn_
Cuts at: 0 166 1233
Size: 166 1067
SfcI C'TryA_G
Cuts at: 0 67 558 603 715 889 1026 1233
Size: 67 491 45 112 174 137 207
Fragments arranged by size:
491 207 174 137 112 67 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 808 1233
Size: 808 425
SmaI CCC'GGG
Cuts at: 0 799 1233
Size: 799 434
SmlI C'TyrA_G
Cuts at: 0 457 1233
Size: 457 776
StyI C'CwwG_G
Cuts at: 0 80 592 1062 1233
Size: 80 512 470 171
Fragments arranged by size:
512 470 171 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 93 1048 1233
Size: 93 955 185
Fragments arranged by size:
955 185 93
TatI w'GTAC_w
Cuts at: 0 977 1233
Size: 977 256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 649 681 1233
Size: 649 32 552
Fragments arranged by size:
649 552 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 83 87 1060 1212 1233
Size: 83 4 973 152 21
Fragments arranged by size:
973 152 83 21 4
XmaI C'CCGG_G
Cuts at: 0 797 1233
Size: 797 436
XmnI GAAnn'nnTTC
Cuts at: 0 691 1233
Size: 691 542
ZraI GAC'GTC
Cuts at: 0 1110 1233
Size: 1110 123
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AlwNI
ApaI AvaI BaeI BamHI BanI BanII BbsI BciVI
BclI BglI Bme1580I BmtI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BstEII BstYI Bsu36I BtgI
BtgZI DrdI EaeI EarI EciI Eco57MI EcoO109I FseI
HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NheI NruI NspI PasI PciI PflMI
PfoI PpuMI PspOMI PstI PvuII SapI SfcI SfiI
SmaI SmlI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AleI AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BbeI BbvCI BcgI
BfrBI BglII BlpI BmgBI BmrI BplI BpmI Bpu10I
BsaAI BsgI BsiEI BsiWI BsmBI BspEI BsrDI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtsI ClaI CspCI
DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
NarI NdeI NotI NsiI PacI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI