(Linear) (Six-base) MAPSORT of: KIEE0533.seq  Check: 5740  from: 1  to: 618



KIEE0533.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     584     618 
   Size:        373     211      34

  Fragments arranged by size: 

                 373     211      34
 
AfeI AGC'GCT

Cuts at:      0     199     618 
   Size:        199     419
 
AleI CACnn'nnGTG

Cuts at:      0     589     601     618 
   Size:        589      12      17

  Fragments arranged by size: 

                 589      17      12
 
BamHI G'GATC_C

Cuts at:      0     492     618 
   Size:        492     126
 
BanII G_rGCy'C

Cuts at:      0     317     345     618 
   Size:        317      28     273

  Fragments arranged by size: 

                 317     273      28
 
BbvCI CC'TCA_GC

Cuts at:      0     476     618 
   Size:        476     142
 
BmtI G_CTAG'C

Cuts at:      0      34     618 
   Size:         34     584
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     359     618 
   Size:        359     259
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460     618 
   Size:        460     158
 
Bpu10I CC'TnA_GC

Cuts at:      0     476     618 
   Size:        476     142
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     618 
   Size:        221     397
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     340     351     618 
   Size:        340      11     267

  Fragments arranged by size: 

                 340     267      11
 
BseYI C'CCAG_C

Cuts at:      0     425     618 
   Size:        425     193
 
BsiHKAI G_wGCw'C

Cuts at:      0     317     345     618 
   Size:        317      28     273

  Fragments arranged by size: 

                 317     273      28
 
Bsp1286I G_dGCh'C

Cuts at:      0     317     345     618 
   Size:        317      28     273

  Fragments arranged by size: 

                 317     273      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     560     618 
   Size:        560      58
 
BsrBI CCG'CTC

Cuts at:      0     300     618 
   Size:        300     318
 
BsrGI T'GTAC_A

Cuts at:      0     368     593     618 
   Size:        368     225      25

  Fragments arranged by size: 

                 368     225      25
 
BstBI TT'CG_AA

Cuts at:      0     150     618 
   Size:        150     468
 
BstYI r'GATC_y

Cuts at:      0     168     212     492     618 
   Size:        168      44     280     126

  Fragments arranged by size: 

                 280     168     126      44
 
Bsu36I CC'TnA_GG

Cuts at:      0     305     618 
   Size:        305     313
 
EarI CTCTTCn'nnn_

Cuts at:      0     152     618 
   Size:        152     466
 
EcoICRI GAG'CTC

Cuts at:      0     315     343     618 
   Size:        315      28     275

  Fragments arranged by size: 

                 315     275      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     460     584     618 
   Size:        373      87     124      34

  Fragments arranged by size: 

                 373     124      87      34
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     216     381     618 
   Size:        216     165     237

  Fragments arranged by size: 

                 237     216     165
 
HaeII r_GCGC'y

Cuts at:      0     201     618 
   Size:        201     417
 
MslI CAynn'nnrTG

Cuts at:      0     130     589     601     618 
   Size:        130     459      12      17

  Fragments arranged by size: 

                 459     130      17      12
 
NheI G'CTAG_C

Cuts at:      0      30     618 
   Size:         30     588
 
SacI G_AGCT'C

Cuts at:      0     317     345     618 
   Size:        317      28     273

  Fragments arranged by size: 

                 317     273      28
 
SmlI C'TyrA_G

Cuts at:      0     236     618 
   Size:        236     382
 
StyI C'CwwG_G

Cuts at:      0     587     618 
   Size:        587      31
 
TatI w'GTAC_w

Cuts at:      0     368     593     618 
   Size:        368     225      25

  Fragments arranged by size: 

                 368     225      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     527     559     618 
   Size:        527      32      59

  Fragments arranged by size: 

                 527      59      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     125     146     618 
   Size:        125      21     472

  Fragments arranged by size: 

                 472     125      21
 

 Enzymes that do cut:

     AcuI     AfeI     AleI    BamHI    BanII    BbvCI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrGI    BstBI    BstYI   Bsu36I     EarI  EcoICRI  Eco57MI
     FalI    HaeII     MslI     NheI     SacI     SmlI     StyI     TatI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI