(Linear) (Six-base) MAPSORT of: KIEE0536.seq  Check: 6651  from: 1  to: 813



KIEE0536.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AccI GT'mk_AC

Cuts at:      0     169     813 
   Size:        169     644
 
ApoI r'AATT_y

Cuts at:      0      16     179     595     813 
   Size:         16     163     416     218

  Fragments arranged by size: 

                 416     218     163      16
 
AvaI C'yCGr_G

Cuts at:      0     229     813 
   Size:        229     584
 
BanII G_rGCy'C

Cuts at:      0     238     813 
   Size:        238     575
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     479     728     746     813 
   Size:        479     249      18      67

  Fragments arranged by size: 

                 479     249      67      18
 
BclI T'GATC_A

Cuts at:      0       8     813 
   Size:          8     805
 
BmgBI CAC'GTC

Cuts at:      0     797     813 
   Size:        797      16
 
BseYI C'CCAG_C

Cuts at:      0     477     813 
   Size:        477     336
 
BsmI GAATG_Cn'

Cuts at:      0     208     270     813 
   Size:        208      62     543

  Fragments arranged by size: 

                 543     208      62
 
Bsp1286I G_dGCh'C

Cuts at:      0     238     813 
   Size:        238     575
 
BspHI T'CATG_A

Cuts at:      0     249     813 
   Size:        249     564
 
BsrGI T'GTAC_A

Cuts at:      0     372     813 
   Size:        372     441
 
BstEII G'GTnAC_C

Cuts at:      0     585     813 
   Size:        585     228
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     395     813 
   Size:        395     418
 
BstYI r'GATC_y

Cuts at:      0     384     813 
   Size:        384     429
 
BstZ17I GTA'TAC

Cuts at:      0     170     813 
   Size:        170     643
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     658     813 
   Size:        658     155
 
ClaI AT'CG_AT

Cuts at:      0     707     813 
   Size:        707     106
 
EcoO109I rG'GnC_Cy

Cuts at:      0     511     520     813 
   Size:        511       9     293

  Fragments arranged by size: 

                 511     293       9
 
EcoRI G'AATT_C

Cuts at:      0      16     813 
   Size:         16     797
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     210     242     813 
   Size:        210      32     571

  Fragments arranged by size: 

                 571     210      32
 
MfeI C'AATT_G

Cuts at:      0     287     813 
   Size:        287     526
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     813 
   Size:        670     143
 
PpuMI rG'GwC_Cy

Cuts at:      0     520     813 
   Size:        520     293
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     765     797     813 
   Size:        765      32      16

  Fragments arranged by size: 

                 765      32      16
 
SanDI GG'GwC_CC

Cuts at:      0     520     813 
   Size:        520     293
 
SfcI C'TryA_G

Cuts at:      0     279     813 
   Size:        279     534
 
StyI C'CwwG_G

Cuts at:      0     239     813 
   Size:        239     574
 
TatI w'GTAC_w

Cuts at:      0     372     813 
   Size:        372     441
 

 Enzymes that do cut:

     AccI     ApoI     AvaI    BanII     BbsI     BclI    BmgBI    BseYI
     BsmI Bsp1286I    BspHI    BsrGI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     ClaI EcoO109I    EcoRI    Hin4I     MfeI     MmeI    PpuMI
     PsrI    SanDI     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   Bsu36I     BtgI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI