Restriction map for
pF1KE0539
(Linear) (Six-base) MAPSORT of: KIEE0539.seq Check: 161 from: 1 to: 1209
KIEE0539.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 0 656 1209
Size: 656 553
AccI GT'mk_AC
Cuts at: 0 179 1209
Size: 179 1030
Acc65I G'GTAC_C
Cuts at: 0 612 1209
Size: 612 597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1195 1209
Size: 432 763 14
Fragments arranged by size:
763 432 14
AflII C'TTAA_G
Cuts at: 0 1093 1209
Size: 1093 116
AleI CACnn'nnGTG
Cuts at: 0 329 968 1209
Size: 329 639 241
Fragments arranged by size:
639 329 241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1066 1209
Size: 1066 143
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 892 924 1209
Size: 892 32 285
Fragments arranged by size:
892 285 32
ApaI G_GGCC'C
Cuts at: 0 591 1209
Size: 591 618
AvaI C'yCGr_G
Cuts at: 0 287 1209
Size: 287 922
BamHI G'GATC_C
Cuts at: 0 190 1209
Size: 190 1019
BanI G'GyrC_C
Cuts at: 0 612 1209
Size: 612 597
BanII G_rGCy'C
Cuts at: 0 591 1077 1209
Size: 591 486 132
Fragments arranged by size:
591 486 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 760 794 1209
Size: 760 34 415
Fragments arranged by size:
760 415 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 136 1209
Size: 136 1073
BclI T'GATC_A
Cuts at: 0 893 1209
Size: 893 316
BlpI GC'TnA_GC
Cuts at: 0 320 1209
Size: 320 889
Bme1580I G_kGCm'C
Cuts at: 0 591 1209
Size: 591 618
BmrI ACTGGGnnnn_n'
Cuts at: 0 594 1209
Size: 594 615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1099 1209
Size: 457 642 110
Fragments arranged by size:
642 457 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1209
Size: 622 587
BsaAI yAC'GTr
Cuts at: 0 276 1209
Size: 276 933
BsaBI GATnn'nnATC
Cuts at: 0 544 1209
Size: 544 665
BsaHI Gr'CG_yC
Cuts at: 0 653 1209
Size: 653 556
BsaWI w'CCGG_w
Cuts at: 0 296 1209
Size: 296 913
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 551 581 892 922 1096 1126 1209
Size: 551 30 311 30 174 30 83
Fragments arranged by size:
551 311 174 83 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1162 1209
Size: 1162 47
BseYI C'CCAG_C
Cuts at: 0 805 1209
Size: 805 404
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1209
Size: 1078 131
BsiHKAI G_wGCw'C
Cuts at: 0 326 1034 1209
Size: 326 708 175
Fragments arranged by size:
708 326 175
BsmI GAATG_Cn'
Cuts at: 0 343 1159 1209
Size: 343 816 50
Fragments arranged by size:
816 343 50
Bsp1286I G_dGCh'C
Cuts at: 0 326 591 1034 1077 1209
Size: 326 265 443 43 132
Fragments arranged by size:
443 326 265 132 43
BspEI T'CCGG_A
Cuts at: 0 296 1209
Size: 296 913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 600 1209
Size: 600 609
BsrBI CCG'CTC
Cuts at: 0 22 1209
Size: 22 1187
BsrDI GCAATG_nn'
Cuts at: 0 485 1209
Size: 485 724
BsrFI r'CCGG_y
Cuts at: 0 117 1209
Size: 117 1092
BssSI C'ACGA_G
Cuts at: 0 779 1209
Size: 779 430
BstEII G'GTnAC_C
Cuts at: 0 663 690 1209
Size: 663 27 519
Fragments arranged by size:
663 519 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 631 1209
Size: 631 578
BstYI r'GATC_y
Cuts at: 0 190 545 1209
Size: 190 355 664
Fragments arranged by size:
664 355 190
Bsu36I CC'TnA_GG
Cuts at: 0 98 1209
Size: 98 1111
BtgI C'CryG_G
Cuts at: 0 248 1125 1142 1209
Size: 248 877 17 67
Fragments arranged by size:
877 248 67 17
DraIII CAC_nnn'GTG
Cuts at: 0 168 1209
Size: 168 1041
EaeI y'GGCC_r
Cuts at: 0 569 861 1122 1128 1209
Size: 569 292 261 6 81
Fragments arranged by size:
569 292 261 81 6
EarI CTCTTCn'nnn_
Cuts at: 0 82 1209
Size: 82 1127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 457 1099 1195 1209
Size: 432 25 642 96 14
Fragments arranged by size:
642 432 96 25 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 745 877 1209
Size: 745 132 332
Fragments arranged by size:
745 332 132
EcoO109I rG'GnC_Cy
Cuts at: 0 35 1209
Size: 35 1174
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 330 402 1209
Size: 330 72 807
Fragments arranged by size:
807 330 72
FspI TGC'GCA
Cuts at: 0 745 1209
Size: 745 464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 1096 1128 1209
Size: 94 32 970 32 81
Fragments arranged by size:
970 94 81 32 32
HincII GTy'rAC
Cuts at: 0 180 1209
Size: 180 1029
KpnI G_GTAC'C
Cuts at: 0 616 1209
Size: 616 593
MscI TGG'CCA
Cuts at: 0 863 1124 1209
Size: 863 261 85
Fragments arranged by size:
863 261 85
MslI CAynn'nnrTG
Cuts at: 0 329 499 661 968 1209
Size: 329 170 162 307 241
Fragments arranged by size:
329 307 241 170 162
MspA1I CmG'CkG
Cuts at: 0 435 1209
Size: 435 774
NcoI C'CATG_G
Cuts at: 0 248 1125 1142 1209
Size: 248 877 17 67
Fragments arranged by size:
877 248 67 17
PflMI CCAn_nnn'nTGG
Cuts at: 0 540 701 1143 1209
Size: 540 161 442 66
Fragments arranged by size:
540 442 161 66
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 892 924 1209
Size: 892 32 285
Fragments arranged by size:
892 285 32
PpuMI rG'GwC_Cy
Cuts at: 0 35 1209
Size: 35 1174
PshAI GACnn'nnGTC
Cuts at: 0 33 1209
Size: 33 1176
PsiI TTA'TAA
Cuts at: 0 854 1209
Size: 854 355
PspOMI G'GGCC_C
Cuts at: 0 587 1209
Size: 587 622
PspXI vC'TCGA_Gb
Cuts at: 0 287 1209
Size: 287 922
PvuII CAG'CTG
Cuts at: 0 435 1209
Size: 435 774
SalI G'TCGA_C
Cuts at: 0 178 1209
Size: 178 1031
SanDI GG'GwC_CC
Cuts at: 0 35 1209
Size: 35 1174
SapI GCTCTTCn'nnn_
Cuts at: 0 82 1209
Size: 82 1127
ScaI AGT'ACT
Cuts at: 0 397 466 1209
Size: 397 69 743
Fragments arranged by size:
743 397 69
SmlI C'TyrA_G
Cuts at: 0 287 601 1093 1209
Size: 287 314 492 116
Fragments arranged by size:
492 314 287 116
StuI AGG'CCT
Cuts at: 0 293 385 740 1209
Size: 293 92 355 469
Fragments arranged by size:
469 355 293 92
StyI C'CwwG_G
Cuts at: 0 194 248 668 1125 1142 1209
Size: 194 54 420 457 17 67
Fragments arranged by size:
457 420 194 67 54 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 511 940 1199 1209
Size: 261 250 429 259 10
Fragments arranged by size:
429 261 259 250 10
TatI w'GTAC_w
Cuts at: 0 395 464 1209
Size: 395 69 745
Fragments arranged by size:
745 395 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 182 214 551 583 1209
Size: 182 32 337 32 626
Fragments arranged by size:
626 337 182 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 988 1209
Size: 988 221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 1209
Size: 255 954
XhoI C'TCGA_G
Cuts at: 0 287 1209
Size: 287 922
XmnI GAAnn'nnTTC
Cuts at: 0 91 445 1159 1209
Size: 91 354 714 50
Fragments arranged by size:
714 354 91 50
ZraI GAC'GTC
Cuts at: 0 654 1209
Size: 654 555
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AleI AlfI AloI
ApaI AvaI BamHI BanI BanII BcgI BciVI BclI
BlpI Bme1580I BmrI BpmI BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI
BstYI Bsu36I BtgI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I FalI FspI Hin4I HincII KpnI MscI MslI
MspA1I NcoI PflMI PpiI PpuMI PshAI PsiI PspOMI
PspXI PvuII SalI SanDI SapI ScaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
AarI AclI AfeI AflIII AgeI AhdI AlwNI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BfrBI BglI BglII BmgBI BmtI BplI Bpu10I
BsaI BsiEI BsiWI BsmBI BspHI BsrGI BssHII BstAPI
BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspAI HaeII
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PsrI PstI PvuI
RsrII SacI SacII SbfI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XmaI