Restriction map for
pF1KE0540
(Linear) (Six-base) MAPSORT of: KIEE0540.seq Check: 1438 from: 1 to: 798
KIEE0540.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 323 426 798
Size: 323 103 372
Fragments arranged by size:
372 323 103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 143 798
Size: 143 655
AlwNI CAG_nnn'CTG
Cuts at: 0 90 463 798
Size: 90 373 335
Fragments arranged by size:
373 335 90
ApaI G_GGCC'C
Cuts at: 0 127 163 798
Size: 127 36 635
Fragments arranged by size:
635 127 36
AvaI C'yCGr_G
Cuts at: 0 505 798
Size: 505 293
BanII G_rGCy'C
Cuts at: 0 22 127 163 339 798
Size: 22 105 36 176 459
Fragments arranged by size:
459 176 105 36 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 480 798
Size: 480 318
BclI T'GATC_A
Cuts at: 0 185 798
Size: 185 613
BglI GCCn_nnn'nGGC
Cuts at: 0 226 235 798
Size: 226 9 563
Fragments arranged by size:
563 226 9
BglII A'GATC_T
Cuts at: 0 746 798
Size: 746 52
BlpI GC'TnA_GC
Cuts at: 0 740 798
Size: 740 58
Bme1580I G_kGCm'C
Cuts at: 0 127 163 798
Size: 127 36 635
Fragments arranged by size:
635 127 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 765 798
Size: 765 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 458 798
Size: 442 16 340
Fragments arranged by size:
442 340 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 798
Size: 34 764
BsaWI w'CCGG_w
Cuts at: 0 57 798
Size: 57 741
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 499 798
Size: 454 45 299
Fragments arranged by size:
454 299 45
BsiEI CG_ry'CG
Cuts at: 0 726 798
Size: 726 72
Bsp1286I G_dGCh'C
Cuts at: 0 22 127 163 339 798
Size: 22 105 36 176 459
Fragments arranged by size:
459 176 105 36 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 323 426 695 798
Size: 323 103 269 103
Fragments arranged by size:
323 269 103 103
BsrBI CCG'CTC
Cuts at: 0 510 798
Size: 510 288
BsrDI GCAATG_nn'
Cuts at: 0 674 798
Size: 674 124
BsrFI r'CCGG_y
Cuts at: 0 574 631 798
Size: 574 57 167
Fragments arranged by size:
574 167 57
BstAPI GCAn_nnn'nTGC
Cuts at: 0 441 798
Size: 441 357
BstBI TT'CG_AA
Cuts at: 0 171 798
Size: 171 627
BstXI CCAn_nnnn'nTGG
Cuts at: 0 163 798
Size: 163 635
BstYI r'GATC_y
Cuts at: 0 746 798
Size: 746 52
Bsu36I CC'TnA_GG
Cuts at: 0 232 798
Size: 232 566
BtgI C'CryG_G
Cuts at: 0 527 798
Size: 527 271
BtsI GCAGTG_nn'
Cuts at: 0 393 792 798
Size: 393 399 6
Fragments arranged by size:
399 393 6
DrdI GACnn_nn'nnGTC
Cuts at: 0 566 798
Size: 566 232
EaeI y'GGCC_r
Cuts at: 0 723 798
Size: 723 75
EagI C'GGCC_G
Cuts at: 0 723 798
Size: 723 75
EarI CTCTTCn'nnn_
Cuts at: 0 712 798
Size: 712 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 458 798
Size: 442 16 340
Fragments arranged by size:
442 340 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 283 798
Size: 283 515
EcoO109I rG'GnC_Cy
Cuts at: 0 71 87 101 123 124 159 160 218
Size: 71 16 14 22 1 35 1 58
Cuts at: 218 798
Size: 580
Fragments arranged by size:
580 71 58 35 22 16 14 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 369 798
Size: 369 429
MslI CAynn'nnrTG
Cuts at: 0 496 798
Size: 496 302
MspA1I CmG'CkG
Cuts at: 0 292 450 798
Size: 292 158 348
Fragments arranged by size:
348 292 158
NaeI GCC'GGC
Cuts at: 0 633 798
Size: 633 165
NcoI C'CATG_G
Cuts at: 0 527 798
Size: 527 271
NgoMIV G'CCGG_C
Cuts at: 0 631 798
Size: 631 167
PasI CC'CwG_GG
Cuts at: 0 67 75 156 163 798
Size: 67 8 81 7 635
Fragments arranged by size:
635 81 67 8 7
PflMI CCAn_nnn'nTGG
Cuts at: 0 29 318 798
Size: 29 289 480
Fragments arranged by size:
480 289 29
PfoI T'CCnGG_A
Cuts at: 0 542 798
Size: 542 256
PpuMI rG'GwC_Cy
Cuts at: 0 71 101 798
Size: 71 30 697
Fragments arranged by size:
697 71 30
PspOMI G'GGCC_C
Cuts at: 0 123 159 798
Size: 123 36 639
Fragments arranged by size:
639 123 36
PstI C_TGCA'G
Cuts at: 0 132 257 798
Size: 132 125 541
Fragments arranged by size:
541 132 125
PvuII CAG'CTG
Cuts at: 0 292 450 798
Size: 292 158 348
Fragments arranged by size:
348 292 158
SanDI GG'GwC_CC
Cuts at: 0 71 798
Size: 71 727
SfcI C'TryA_G
Cuts at: 0 128 253 798
Size: 128 125 545
Fragments arranged by size:
545 128 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 226 798
Size: 226 572
SmlI C'TyrA_G
Cuts at: 0 49 798
Size: 49 749
StuI AGG'CCT
Cuts at: 0 194 798
Size: 194 604
StyI C'CwwG_G
Cuts at: 0 527 638 798
Size: 527 111 160
Fragments arranged by size:
527 160 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 357 569 798
Size: 357 212 229
Fragments arranged by size:
357 229 212
TatI w'GTAC_w
Cuts at: 0 377 620 798
Size: 377 243 178
Fragments arranged by size:
377 243 178
Enzymes that do cut:
AarI AlfI AlwNI ApaI AvaI BanII BbsI BclI
BglI BglII BlpI Bme1580I BplI BpmI BpuEI BsaWI
BsgI BsiEI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EagI EarI Eco57MI EcoNI EcoO109I FalI MslI MspA1I
NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SanDI SfcI SfiI SmlI StuI StyI
TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BmgBI BmrI BmtI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BssHII BssSI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NarI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI