Restriction map for
pF1KE0541
(Linear) (Six-base) MAPSORT of: KIEE0541.seq Check: 9649 from: 1 to: 663
KIEE0541.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 663
Size: 145 518
AgeI A'CCGG_T
Cuts at: 0 91 663
Size: 91 572
AleI CACnn'nnGTG
Cuts at: 0 322 502 663
Size: 322 180 161
Fragments arranged by size:
322 180 161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 663
Size: 358 305
BamHI G'GATC_C
Cuts at: 0 181 248 663
Size: 181 67 415
Fragments arranged by size:
415 181 67
BanI G'GyrC_C
Cuts at: 0 79 272 496 663
Size: 79 193 224 167
Fragments arranged by size:
224 193 167 79
BanII G_rGCy'C
Cuts at: 0 104 641 663
Size: 104 537 22
Fragments arranged by size:
537 104 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 663
Size: 410 253
Bme1580I G_kGCm'C
Cuts at: 0 277 499 663
Size: 277 222 164
Fragments arranged by size:
277 222 164
BmrI ACTGGGnnnn_n'
Cuts at: 0 468 569 663
Size: 468 101 94
Fragments arranged by size:
468 101 94
BsaAI yAC'GTr
Cuts at: 0 596 663
Size: 596 67
BsaHI Gr'CG_yC
Cuts at: 0 220 230 663
Size: 220 10 433
Fragments arranged by size:
433 220 10
BsaWI w'CCGG_w
Cuts at: 0 91 663
Size: 91 572
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 663
Size: 256 30 377
Fragments arranged by size:
377 256 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 328 663
Size: 142 186 335
Fragments arranged by size:
335 186 142
BsiHKAI G_wGCw'C
Cuts at: 0 104 663
Size: 104 559
Bsp1286I G_dGCh'C
Cuts at: 0 104 277 499 641 663
Size: 104 173 222 142 22
Fragments arranged by size:
222 173 142 104 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 118 350 637 663
Size: 118 232 287 26
Fragments arranged by size:
287 232 118 26
BsrFI r'CCGG_y
Cuts at: 0 33 91 208 663
Size: 33 58 117 455
Fragments arranged by size:
455 117 58 33
BssSI C'ACGA_G
Cuts at: 0 103 663
Size: 103 560
BstEII G'GTnAC_C
Cuts at: 0 204 663
Size: 204 459
BstYI r'GATC_y
Cuts at: 0 181 248 663
Size: 181 67 415
Fragments arranged by size:
415 181 67
BtgI C'CryG_G
Cuts at: 0 15 252 546 564 605 663
Size: 15 237 294 18 41 58
Fragments arranged by size:
294 237 58 41 18 15
DraIII CAC_nnn'GTG
Cuts at: 0 150 663
Size: 150 513
DrdI GACnn_nn'nnGTC
Cuts at: 0 114 663
Size: 114 549
EaeI y'GGCC_r
Cuts at: 0 31 192 663
Size: 31 161 471
Fragments arranged by size:
471 161 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 261 663
Size: 261 402
EcoICRI GAG'CTC
Cuts at: 0 102 663
Size: 102 561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 663
Size: 145 518
EcoO109I rG'GnC_Cy
Cuts at: 0 284 663
Size: 284 379
HaeII r_GCGC'y
Cuts at: 0 61 663
Size: 61 602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 398 663
Size: 203 195 265
Fragments arranged by size:
265 203 195
MslI CAynn'nnrTG
Cuts at: 0 322 502 663
Size: 322 180 161
Fragments arranged by size:
322 180 161
MspA1I CmG'CkG
Cuts at: 0 17 191 254 369 663
Size: 17 174 63 115 294
Fragments arranged by size:
294 174 115 63 17
NcoI C'CATG_G
Cuts at: 0 605 663
Size: 605 58
NspI r_CATG'y
Cuts at: 0 325 493 573 663
Size: 325 168 80 90
Fragments arranged by size:
325 168 90 80
PflMI CCAn_nnn'nTGG
Cuts at: 0 492 663
Size: 492 171
PpuMI rG'GwC_Cy
Cuts at: 0 284 663
Size: 284 379
PstI C_TGCA'G
Cuts at: 0 648 663
Size: 648 15
SacI G_AGCT'C
Cuts at: 0 104 663
Size: 104 559
SacII CC_GC'GG
Cuts at: 0 18 255 663
Size: 18 237 408
Fragments arranged by size:
408 237 18
SanDI GG'GwC_CC
Cuts at: 0 284 663
Size: 284 379
SfcI C'TryA_G
Cuts at: 0 644 663
Size: 644 19
SphI G_CATG'C
Cuts at: 0 573 663
Size: 573 90
StyI C'CwwG_G
Cuts at: 0 605 663
Size: 605 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 256 288 663
Size: 256 32 375
Fragments arranged by size:
375 256 32
Enzymes that do cut:
AcuI AgeI AleI AlfI BamHI BanI BanII BbsI
Bme1580I BmrI BsaAI BsaHI BsaWI BsaXI BsgI BsiHKAI
Bsp1286I BspMI BsrFI BssSI BstEII BstYI BtgI DraIII
DrdI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII MmeI
MslI MspA1I NcoI NspI PflMI PpuMI PstI SacI
SacII SanDI SfcI SphI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BseRI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI EagI EarI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI