(Linear) (Six-base) MAPSORT of: KIEE0542.seq  Check: 9613  from: 1  to: 921



KIEE0542.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     406     921 
   Size:         94     312     515

  Fragments arranged by size: 

                 515     312      94
 
AleI CACnn'nnGTG

Cuts at:      0     652     921 
   Size:        652     269
 
AlwNI CAG_nnn'CTG

Cuts at:      0     224     921 
   Size:        224     697
 
ApaLI G'TGCA_C

Cuts at:      0     908     921 
   Size:        908      13
 
ApoI r'AATT_y

Cuts at:      0     403     921 
   Size:        403     518
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     426     459     921 
   Size:        426      33     462

  Fragments arranged by size: 

                 462     426      33
 
BanI G'GyrC_C

Cuts at:      0     186     921 
   Size:        186     735
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     422     519     730     921 
   Size:        422      97     211     191

  Fragments arranged by size: 

                 422     211     191      97
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      84     921 
   Size:         84     837
 
BglII A'GATC_T

Cuts at:      0     309     921 
   Size:        309     612
 
Bme1580I G_kGCm'C

Cuts at:      0     912     921 
   Size:        912       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     921 
   Size:        178     743
 
BsaHI Gr'CG_yC

Cuts at:      0     699     921 
   Size:        699     222
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     647     921 
   Size:        647     274
 
BsiHKAI G_wGCw'C

Cuts at:      0     155     912     921 
   Size:        155     757       9

  Fragments arranged by size: 

                 757     155       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     912     921 
   Size:        155     757       9

  Fragments arranged by size: 

                 757     155       9
 
BsrDI GCAATG_nn'

Cuts at:      0      74     514     921 
   Size:         74     440     407

  Fragments arranged by size: 

                 440     407      74
 
BstEII G'GTnAC_C

Cuts at:      0     654     921 
   Size:        654     267
 
BstYI r'GATC_y

Cuts at:      0     309     921 
   Size:        309     612
 
BtgI C'CryG_G

Cuts at:      0      38      50     650     921 
   Size:         38      12     600     271

  Fragments arranged by size: 

                 600     271      38      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     488     921 
   Size:        488     433
 
BtsI GCAGTG_nn'

Cuts at:      0     836     921 
   Size:        836      85
 
DraI TTT'AAA

Cuts at:      0     566     581     921 
   Size:        566      15     340

  Fragments arranged by size: 

                 566     340      15
 
DraIII CAC_nnn'GTG

Cuts at:      0      39     150     921 
   Size:         39     111     771

  Fragments arranged by size: 

                 771     111      39
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     678     921 
   Size:        678     243
 
EaeI y'GGCC_r

Cuts at:      0      41     921 
   Size:         41     880
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     178     406     921 
   Size:         94      84     228     515

  Fragments arranged by size: 

                 515     228      94      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     204     236     921 
   Size:        204      32     685

  Fragments arranged by size: 

                 685     204      32
 
HincII GTy'rAC

Cuts at:      0     289     921 
   Size:        289     632
 
HindIII A'AGCT_T

Cuts at:      0     388     595     921 
   Size:        388     207     326

  Fragments arranged by size: 

                 388     326     207
 
MslI CAynn'nnrTG

Cuts at:      0     652     892     921 
   Size:        652     240      29

  Fragments arranged by size: 

                 652     240      29
 
MspA1I CmG'CkG

Cuts at:      0     877     921 
   Size:        877      44
 
NruI TCG'CGA

Cuts at:      0     473     921 
   Size:        473     448
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      39     921 
   Size:         39     882
 
PfoI T'CCnGG_A

Cuts at:      0     155     921 
   Size:        155     766
 
PstI C_TGCA'G

Cuts at:      0     671     827     921 
   Size:        671     156      94

  Fragments arranged by size: 

                 671     156      94
 
PvuII CAG'CTG

Cuts at:      0     877     921 
   Size:        877      44
 
SfcI C'TryA_G

Cuts at:      0     667     823     921 
   Size:        667     156      98

  Fragments arranged by size: 

                 667     156      98
 
SspI AAT'ATT

Cuts at:      0     747     921 
   Size:        747     174
 
StuI AGG'CCT

Cuts at:      0     806     921 
   Size:        806     115
 
StyI C'CwwG_G

Cuts at:      0     605     921 
   Size:        605     316
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     357     921 
   Size:        357     564
 
TatI w'GTAC_w

Cuts at:      0     675     921 
   Size:        675     246
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     197     921 
   Size:        197     724
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI    ApaLI     ApoI     BaeI     BanI     BbsI
    BciVI    BglII Bme1580I     BpmI    BsaHI     BsgI  BsiHKAI Bsp1286I
    BsrDI   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI  Eco57MI    Hin4I   HincII  HindIII     MslI   MspA1I
     NruI    PflMI     PfoI     PstI    PvuII     SfcI     SspI     StuI
     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI    BanII     BbeI    BbvCI     BcgI     BclI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI