Restriction map for
pF1KE0543
(Linear) (Six-base) MAPSORT of: KIEE0543.seq Check: 4557 from: 1 to: 945
KIEE0543.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 945
Size: 325 620
AflIII A'CryG_T
Cuts at: 0 776 945
Size: 776 169
AlwNI CAG_nnn'CTG
Cuts at: 0 15 534 945
Size: 15 519 411
Fragments arranged by size:
519 411 15
AvaI C'yCGr_G
Cuts at: 0 167 945
Size: 167 778
BanI G'GyrC_C
Cuts at: 0 485 559 945
Size: 485 74 386
Fragments arranged by size:
485 386 74
BanII G_rGCy'C
Cuts at: 0 59 427 545 644 945
Size: 59 368 118 99 301
Fragments arranged by size:
368 301 118 99 59
BbeI G_GCGC'C
Cuts at: 0 489 945
Size: 489 456
BbsI GAAGACnn'nnnn_
Cuts at: 0 166 945
Size: 166 779
BbvCI CC'TCA_GC
Cuts at: 0 432 945
Size: 432 513
BglI GCCn_nnn'nGGC
Cuts at: 0 652 945
Size: 652 293
Bme1580I G_kGCm'C
Cuts at: 0 864 945
Size: 864 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 432 945
Size: 212 220 513
Fragments arranged by size:
513 220 212
Bpu10I CC'TnA_GC
Cuts at: 0 432 945
Size: 432 513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 739 878 945
Size: 361 378 139 67
Fragments arranged by size:
378 361 139 67
BsaHI Gr'CG_yC
Cuts at: 0 402 486 655 718 945
Size: 402 84 169 63 227
Fragments arranged by size:
402 227 169 84 63
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 945
Size: 559 386
BseYI C'CCAG_C
Cuts at: 0 334 538 945
Size: 334 204 407
Fragments arranged by size:
407 334 204
BsiEI CG_ry'CG
Cuts at: 0 710 945
Size: 710 235
BsiHKAI G_wGCw'C
Cuts at: 0 644 945
Size: 644 301
Bsp1286I G_dGCh'C
Cuts at: 0 59 427 545 644 864 945
Size: 59 368 118 99 220 81
Fragments arranged by size:
368 220 118 99 81 59
BspHI T'CATG_A
Cuts at: 0 839 945
Size: 839 106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 79 682 945
Size: 79 603 263
Fragments arranged by size:
603 263 79
BsrDI GCAATG_nn'
Cuts at: 0 503 945
Size: 503 442
BsrFI r'CCGG_y
Cuts at: 0 646 945
Size: 646 299
BsrGI T'GTAC_A
Cuts at: 0 131 779 945
Size: 131 648 166
Fragments arranged by size:
648 166 131
BssSI C'ACGA_G
Cuts at: 0 58 945
Size: 58 887
BstEII G'GTnAC_C
Cuts at: 0 66 354 945
Size: 66 288 591
Fragments arranged by size:
591 288 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 246 945
Size: 90 156 699
Fragments arranged by size:
699 156 90
BstYI r'GATC_y
Cuts at: 0 12 945
Size: 12 933
BtgI C'CryG_G
Cuts at: 0 365 945
Size: 365 580
BtsI GCAGTG_nn'
Cuts at: 0 212 719 945
Size: 212 507 226
Fragments arranged by size:
507 226 212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 486 521 945
Size: 486 35 424
Fragments arranged by size:
486 424 35
EaeI y'GGCC_r
Cuts at: 0 92 251 407 945
Size: 92 159 156 538
Fragments arranged by size:
538 159 156 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 187 945
Size: 187 758
EcoICRI GAG'CTC
Cuts at: 0 642 945
Size: 642 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 325 432 945
Size: 212 113 107 513
Fragments arranged by size:
513 212 113 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 769 945
Size: 769 176
EcoO109I rG'GnC_Cy
Cuts at: 0 452 531 552 945
Size: 452 79 21 393
Fragments arranged by size:
452 393 79 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 675 945
Size: 675 270
HaeII r_GCGC'y
Cuts at: 0 489 851 945
Size: 489 362 94
Fragments arranged by size:
489 362 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 814 846 911 943 945
Size: 814 32 65 32 2
Fragments arranged by size:
814 65 32 32 2
KasI G'GCGC_C
Cuts at: 0 485 945
Size: 485 460
MscI TGG'CCA
Cuts at: 0 94 945
Size: 94 851
MslI CAynn'nnrTG
Cuts at: 0 370 945
Size: 370 575
MspA1I CmG'CkG
Cuts at: 0 297 387 945
Size: 297 90 558
Fragments arranged by size:
558 297 90
NarI GG'CG_CC
Cuts at: 0 486 945
Size: 486 459
NcoI C'CATG_G
Cuts at: 0 365 945
Size: 365 580
NspI r_CATG'y
Cuts at: 0 192 780 945
Size: 192 588 165
Fragments arranged by size:
588 192 165
PciI A'CATG_T
Cuts at: 0 776 945
Size: 776 169
PflMI CCAn_nnn'nTGG
Cuts at: 0 351 945
Size: 351 594
PpuMI rG'GwC_Cy
Cuts at: 0 452 945
Size: 452 493
PstI C_TGCA'G
Cuts at: 0 110 207 392 945
Size: 110 97 185 553
Fragments arranged by size:
553 185 110 97
PvuII CAG'CTG
Cuts at: 0 387 945
Size: 387 558
SacI G_AGCT'C
Cuts at: 0 644 945
Size: 644 301
SfcI C'TryA_G
Cuts at: 0 106 203 388 546 945
Size: 106 97 185 158 399
Fragments arranged by size:
399 185 158 106 97
SfoI GGC'GCC
Cuts at: 0 487 945
Size: 487 458
SmlI C'TyrA_G
Cuts at: 0 376 754 893 945
Size: 376 378 139 52
Fragments arranged by size:
378 376 139 52
StuI AGG'CCT
Cuts at: 0 764 945
Size: 764 181
StyI C'CwwG_G
Cuts at: 0 365 945
Size: 365 580
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 493 547 945
Size: 493 54 398
Fragments arranged by size:
493 398 54
TatI w'GTAC_w
Cuts at: 0 131 779 945
Size: 131 648 166
Fragments arranged by size:
648 166 131
XbaI T'CTAG_A
Cuts at: 0 723 945
Size: 723 222
Enzymes that do cut:
AcuI AflIII AlwNI AvaI BanI BanII BbeI BbsI
BbvCI BglI Bme1580I BpmI Bpu10I BpuEI BsaHI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstEII BstXI BstYI BtgI BtsI CspCI
EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MscI MslI MspA1I NarI NcoI NspI
PciI PflMI PpuMI PstI PvuII SacI SfcI SfoI
SmlI StuI StyI TaqII TatI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaI
BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BsmBI
BspEI BsrBI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EagI EarI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI