Restriction map for
pF1KE0547
(Linear) (Six-base) MAPSORT of: KIEE0547.seq Check: 5113 from: 1 to: 1269
KIEE0547.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 979 1269
Size: 898 81 290
Fragments arranged by size:
898 290 81
AflIII A'CryG_T
Cuts at: 0 635 704 1269
Size: 635 69 565
Fragments arranged by size:
635 565 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 691 723 1269
Size: 691 32 546
Fragments arranged by size:
691 546 32
AlwNI CAG_nnn'CTG
Cuts at: 0 401 876 1209 1269
Size: 401 475 333 60
Fragments arranged by size:
475 401 333 60
ApaI G_GGCC'C
Cuts at: 0 1217 1269
Size: 1217 52
ApoI r'AATT_y
Cuts at: 0 102 1269
Size: 102 1167
AseI AT'TA_AT
Cuts at: 0 1043 1269
Size: 1043 226
AvaI C'yCGr_G
Cuts at: 0 482 681 1239 1269
Size: 482 199 558 30
Fragments arranged by size:
558 482 199 30
BanI G'GyrC_C
Cuts at: 0 888 1269
Size: 888 381
BanII G_rGCy'C
Cuts at: 0 391 1217 1269
Size: 391 826 52
Fragments arranged by size:
826 391 52
BbeI G_GCGC'C
Cuts at: 0 892 1269
Size: 892 377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 825 859 964 998 1211 1245 1269
Size: 825 34 105 34 213 34 24
Fragments arranged by size:
825 213 105 34 34 34 24
BclI T'GATC_A
Cuts at: 0 47 1269
Size: 47 1222
Bme1580I G_kGCm'C
Cuts at: 0 1217 1269
Size: 1217 52
BmgBI CAC'GTC
Cuts at: 0 1001 1269
Size: 1001 268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 826 1269
Size: 283 543 443
Fragments arranged by size:
543 443 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1269
Size: 1012 257
BsaAI yAC'GTr
Cuts at: 0 534 614 1269
Size: 534 80 655
Fragments arranged by size:
655 534 80
BsaHI Gr'CG_yC
Cuts at: 0 889 1269
Size: 889 380
BsaWI w'CCGG_w
Cuts at: 0 380 1269
Size: 380 889
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 1269
Size: 388 881
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 889 1269
Size: 648 241 380
Fragments arranged by size:
648 380 241
BsiEI CG_ry'CG
Cuts at: 0 1231 1269
Size: 1231 38
BsiHKAI G_wGCw'C
Cuts at: 0 814 1269
Size: 814 455
BsmI GAATG_Cn'
Cuts at: 0 928 987 1269
Size: 928 59 282
Fragments arranged by size:
928 282 59
BsmBI CGTCTCn'nnnn_
Cuts at: 0 998 1269
Size: 998 271
Bsp1286I G_dGCh'C
Cuts at: 0 391 814 1217 1269
Size: 391 423 403 52
Fragments arranged by size:
423 403 391 52
BspEI T'CCGG_A
Cuts at: 0 380 1269
Size: 380 889
BspHI T'CATG_A
Cuts at: 0 407 1269
Size: 407 862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 596 800 1269
Size: 596 204 469
Fragments arranged by size:
596 469 204
BsrBI CCG'CTC
Cuts at: 0 1249 1269
Size: 1249 20
BsrFI r'CCGG_y
Cuts at: 0 656 891 1269
Size: 656 235 378
Fragments arranged by size:
656 378 235
BsrGI T'GTAC_A
Cuts at: 0 638 1269
Size: 638 631
BstEII G'GTnAC_C
Cuts at: 0 828 1269
Size: 828 441
BstXI CCAn_nnnn'nTGG
Cuts at: 0 696 766 1269
Size: 696 70 503
Fragments arranged by size:
696 503 70
BstYI r'GATC_y
Cuts at: 0 851 1269
Size: 851 418
Bsu36I CC'TnA_GG
Cuts at: 0 1112 1269
Size: 1112 157
BtgI C'CryG_G
Cuts at: 0 765 816 1269
Size: 765 51 453
Fragments arranged by size:
765 453 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1044 1269
Size: 1044 225
BtsI GCAGTG_nn'
Cuts at: 0 622 912 1007 1269
Size: 622 290 95 262
Fragments arranged by size:
622 290 262 95
DrdI GACnn_nn'nnGTC
Cuts at: 0 181 1269
Size: 181 1088
EarI CTCTTCn'nnn_
Cuts at: 0 379 1269
Size: 379 890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 826 898 979 1269
Size: 283 543 72 81 290
Fragments arranged by size:
543 290 283 81 72
EcoO109I rG'GnC_Cy
Cuts at: 0 222 1213 1269
Size: 222 991 56
Fragments arranged by size:
991 222 56
EcoRV GAT'ATC
Cuts at: 0 30 160 1269
Size: 30 130 1109
Fragments arranged by size:
1109 130 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 459 933 1269
Size: 459 474 336
Fragments arranged by size:
474 459 336
HaeII r_GCGC'y
Cuts at: 0 892 1269
Size: 892 377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 59 91 149 181 1179 1211 1269
Size: 59 32 58 32 998 32 58
Fragments arranged by size:
998 59 58 58 32 32 32
HincII GTy'rAC
Cuts at: 0 186 1269
Size: 186 1083
HindIII A'AGCT_T
Cuts at: 0 643 1269
Size: 643 626
KasI G'GCGC_C
Cuts at: 0 888 1269
Size: 888 381
MslI CAynn'nnrTG
Cuts at: 0 340 764 1269
Size: 340 424 505
Fragments arranged by size:
505 424 340
MspA1I CmG'CkG
Cuts at: 0 352 818 1269
Size: 352 466 451
Fragments arranged by size:
466 451 352
NarI GG'CG_CC
Cuts at: 0 889 1269
Size: 889 380
NcoI C'CATG_G
Cuts at: 0 765 1269
Size: 765 504
NspI r_CATG'y
Cuts at: 0 639 708 1269
Size: 639 69 561
Fragments arranged by size:
639 561 69
PasI CC'CwG_GG
Cuts at: 0 429 1161 1269
Size: 429 732 108
Fragments arranged by size:
732 429 108
PciI A'CATG_T
Cuts at: 0 635 704 1269
Size: 635 69 565
Fragments arranged by size:
635 565 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 401 1269
Size: 401 868
PpuMI rG'GwC_Cy
Cuts at: 0 222 1269
Size: 222 1047
PspOMI G'GGCC_C
Cuts at: 0 1213 1269
Size: 1213 56
PvuI CG_AT'CG
Cuts at: 0 1231 1269
Size: 1231 38
SacII CC_GC'GG
Cuts at: 0 819 1269
Size: 819 450
SapI GCTCTTCn'nnn_
Cuts at: 0 379 1269
Size: 379 890
SfoI GGC'GCC
Cuts at: 0 890 1269
Size: 890 379
SgrAI Cr'CCGG_yG
Cuts at: 0 891 1269
Size: 891 378
SmaI CCC'GGG
Cuts at: 0 484 1269
Size: 484 785
SmlI C'TyrA_G
Cuts at: 0 1027 1269
Size: 1027 242
StyI C'CwwG_G
Cuts at: 0 765 1269
Size: 765 504
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1034 1269
Size: 1034 235
TatI w'GTAC_w
Cuts at: 0 638 1031 1269
Size: 638 393 238
Fragments arranged by size:
638 393 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 603 635 1269
Size: 603 32 634
Fragments arranged by size:
634 603 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1091 1269
Size: 1091 178
XmaI C'CCGG_G
Cuts at: 0 482 1269
Size: 482 787
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApaI ApoI AseI AvaI
BanI BanII BbeI BcgI BclI Bme1580I BmgBI BpmI
BpuEI BsaAI BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
HincII HindIII KasI MslI MspA1I NarI NcoI NspI
PasI PciI PflMI PpuMI PspOMI PvuI SacII SapI
SfoI SgrAI SmaI SmlI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaLI AscI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BfrBI BglI BglII BlpI
BmrI BmtI BplI Bpu10I BsaI BsaBI BsaXI BseYI
BsiWI BsrDI BssHII BssSI BstAPI BstBI BstZ17I ClaI
CspCI DraI DraIII EaeI EagI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HpaI KpnI MfeI MluI
MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuII RsrII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmnI ZraI