(Linear) (Six-base) MAPSORT of: KIEE0549.seq  Check: 1348  from: 1  to: 462



KIEE0549.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     135     398     440     462 
   Size:        135     263      42      22

  Fragments arranged by size: 

                 263     135      42      22
 
ApoI r'AATT_y

Cuts at:      0     403     462 
   Size:        403      59
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     343     377     462 
   Size:        343      34      85

  Fragments arranged by size: 

                 343      85      34
 
BglII A'GATC_T

Cuts at:      0     218     462 
   Size:        218     244
 
Bpu10I CC'TnA_GC

Cuts at:      0     350     462 
   Size:        350     112
 
BseYI C'CCAG_C

Cuts at:      0     344     462 
   Size:        344     118
 
BsiHKAI G_wGCw'C

Cuts at:      0     356     462 
   Size:        356     106
 
BsmI GAATG_Cn'

Cuts at:      0     420     462 
   Size:        420      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     356     462 
   Size:        356     106
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     240     462 
   Size:        240     222
 
BstYI r'GATC_y

Cuts at:      0     218     462 
   Size:        218     244
 
DraI TTT'AAA

Cuts at:      0     210     462 
   Size:        210     252
 
EaeI y'GGCC_r

Cuts at:      0       5     462 
   Size:          5     457
 
EarI CTCTTCn'nnn_

Cuts at:      0     416     462 
   Size:        416      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     135     398     440     462 
   Size:        135     263      42      22

  Fragments arranged by size: 

                 263     135      42      22
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     165     462 
   Size:        165     297
 
NdeI CA'TA_TG

Cuts at:      0     135     462 
   Size:        135     327
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     242     462 
   Size:        242     220
 
XmnI GAAnn'nnTTC

Cuts at:      0      61     462 
   Size:         61     401
 

 Enzymes that do cut:

     AcuI     ApoI     BcgI    BglII   Bpu10I    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BstYI     DraI     EaeI     EarI  Eco57MI     FalI
     NdeI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI