Restriction map for
pF1KE0550
(Linear) (Six-base) MAPSORT of: KIEE0550.seq Check: 6259 from: 1 to: 921
KIEE0550.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 0 625 921
Size: 625 296
AflIII A'CryG_T
Cuts at: 0 14 921
Size: 14 907
ApoI r'AATT_y
Cuts at: 0 216 370 770 784 816 921
Size: 216 154 400 14 32 105
Fragments arranged by size:
400 216 154 105 32 14
BanII G_rGCy'C
Cuts at: 0 388 731 921
Size: 388 343 190
Fragments arranged by size:
388 343 190
BbsI GAAGACnn'nnnn_
Cuts at: 0 33 921
Size: 33 888
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 852 886 921
Size: 852 34 35
Fragments arranged by size:
852 35 34
BglII A'GATC_T
Cuts at: 0 494 921
Size: 494 427
BmgBI CAC'GTC
Cuts at: 0 823 921
Size: 823 98
BmtI G_CTAG'C
Cuts at: 0 94 921
Size: 94 827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 680 773 878 902 921
Size: 268 412 93 105 24 19
Fragments arranged by size:
412 268 105 93 24 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 511 921
Size: 511 410
BsaBI GATnn'nnATC
Cuts at: 0 475 921
Size: 475 446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 70 921
Size: 40 30 851
Fragments arranged by size:
851 40 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 634 921
Size: 634 287
BseYI C'CCAG_C
Cuts at: 0 666 730 921
Size: 666 64 191
Fragments arranged by size:
666 191 64
Bsp1286I G_dGCh'C
Cuts at: 0 388 731 921
Size: 388 343 190
Fragments arranged by size:
388 343 190
BsrDI GCAATG_nn'
Cuts at: 0 895 921
Size: 895 26
BstYI r'GATC_y
Cuts at: 0 494 688 921
Size: 494 194 233
Fragments arranged by size:
494 233 194
BtsI GCAGTG_nn'
Cuts at: 0 542 921
Size: 542 379
DraI TTT'AAA
Cuts at: 0 789 921
Size: 789 132
EaeI y'GGCC_r
Cuts at: 0 356 921
Size: 356 565
EarI CTCTTCn'nnn_
Cuts at: 0 839 921
Size: 839 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 680 773 878 902 921
Size: 268 412 93 105 24 19
Fragments arranged by size:
412 268 105 93 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 921
Size: 328 593
EcoO109I rG'GnC_Cy
Cuts at: 0 97 921
Size: 97 824
EcoRI G'AATT_C
Cuts at: 0 216 770 921
Size: 216 554 151
Fragments arranged by size:
554 216 151
HincII GTy'rAC
Cuts at: 0 345 626 921
Size: 345 281 295
Fragments arranged by size:
345 295 281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 921
Size: 399 522
MscI TGG'CCA
Cuts at: 0 358 921
Size: 358 563
MslI CAynn'nnrTG
Cuts at: 0 562 921
Size: 562 359
MspA1I CmG'CkG
Cuts at: 0 71 921
Size: 71 850
NheI G'CTAG_C
Cuts at: 0 90 921
Size: 90 831
NspI r_CATG'y
Cuts at: 0 18 921
Size: 18 903
PasI CC'CwG_GG
Cuts at: 0 35 582 921
Size: 35 547 339
Fragments arranged by size:
547 339 35
PciI A'CATG_T
Cuts at: 0 14 921
Size: 14 907
PfoI T'CCnGG_A
Cuts at: 0 395 921
Size: 395 526
RsrII CG'GwC_CG
Cuts at: 0 222 921
Size: 222 699
SalI G'TCGA_C
Cuts at: 0 624 921
Size: 624 297
Enzymes that do cut:
AccI AflIII ApoI BanII BbsI BcgI BglII BmgBI
BmtI BpmI BsaI BsaBI BsaXI BseRI BseYI Bsp1286I
BsrDI BstYI BtsI DraI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI HincII MmeI MscI MslI MspA1I NheI
NspI PasI PciI PfoI RsrII SalI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaAI BsaHI BsaWI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI