Restriction map for
pF1KE0557
(Linear) (Six-base) MAPSORT of: KIEE0557.seq Check: 9606 from: 1 to: 1350
KIEE0557.orf
With 182 enzymes: SgfI *
August 18, 2008 14:48 ..
AccI GT'mk_AC
Cuts at: 0 1286 1350
Size: 1286 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1350
Size: 922 428
AhdI GACnn_n'nnGTC
Cuts at: 0 1148 1350
Size: 1148 202
AleI CACnn'nnGTG
Cuts at: 0 539 1350
Size: 539 811
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 802 834 1350
Size: 802 32 516
Fragments arranged by size:
802 516 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1089 1107 1350
Size: 1089 18 243
Fragments arranged by size:
1089 243 18
ApaI G_GGCC'C
Cuts at: 0 1041 1350
Size: 1041 309
ApoI r'AATT_y
Cuts at: 0 99 349 1350
Size: 99 250 1001
Fragments arranged by size:
1001 250 99
AvaI C'yCGr_G
Cuts at: 0 730 1350
Size: 730 620
BanII G_rGCy'C
Cuts at: 0 221 1041 1350
Size: 221 820 309
Fragments arranged by size:
820 309 221
BbvCI CC'TCA_GC
Cuts at: 0 879 1350
Size: 879 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 766 800 1350
Size: 766 34 550
Fragments arranged by size:
766 550 34
BclI T'GATC_A
Cuts at: 0 378 1350
Size: 378 972
BglII A'GATC_T
Cuts at: 0 603 851 1350
Size: 603 248 499
Fragments arranged by size:
603 499 248
Bme1580I G_kGCm'C
Cuts at: 0 1041 1350
Size: 1041 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 340 628 716 988 1063 1111 1350
Size: 319 21 288 88 272 75 48 239
Fragments arranged by size:
319 288 272 239 88 75 48 21
Bpu10I CC'TnA_GC
Cuts at: 0 879 1350
Size: 879 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1297 1350
Size: 493 804 53
Fragments arranged by size:
804 493 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 979 1066 1135 1350
Size: 979 87 69 215
Fragments arranged by size:
979 215 87 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 21 51 601 631 689 719 802 832
Size: 21 30 550 30 58 30 83 30
Cuts at: 832 1350
Size: 518
Fragments arranged by size:
550 518 83 58 30 30 30 30
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 649 1060 1178 1294 1350
Size: 316 333 411 118 116 56
Fragments arranged by size:
411 333 316 118 116 56
BseYI C'CCAG_C
Cuts at: 0 1245 1350
Size: 1245 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 1039 1350
Size: 615 424 311
Fragments arranged by size:
615 424 311
BsiHKAI G_wGCw'C
Cuts at: 0 976 1350
Size: 976 374
Bsp1286I G_dGCh'C
Cuts at: 0 221 976 1041 1350
Size: 221 755 65 309
Fragments arranged by size:
755 309 221 65
BspHI T'CATG_A
Cuts at: 0 381 1350
Size: 381 969
BsrBI CCG'CTC
Cuts at: 0 213 1350
Size: 213 1137
BsrDI GCAATG_nn'
Cuts at: 0 237 456 1350
Size: 237 219 894
Fragments arranged by size:
894 237 219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 681 1350
Size: 681 669
BstEII G'GTnAC_C
Cuts at: 0 240 1350
Size: 240 1110
BstYI r'GATC_y
Cuts at: 0 603 851 1019 1213 1350
Size: 603 248 168 194 137
Fragments arranged by size:
603 248 194 168 137
BtgI C'CryG_G
Cuts at: 0 369 1350
Size: 369 981
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 417 1350
Size: 417 933
BtsI GCAGTG_nn'
Cuts at: 0 691 1350
Size: 691 659
ClaI AT'CG_AT
Cuts at: 0 473 1350
Size: 473 877
EarI CTCTTCn'nnn_
Cuts at: 0 11 536 564 823 920 1325 1350
Size: 11 525 28 259 97 405 25
Fragments arranged by size:
525 405 259 97 28 25 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 340 628 716 922 988 1063 1111
Size: 319 21 288 88 206 66 75 48
Cuts at: 1111 1350
Size: 239
Fragments arranged by size:
319 288 239 206 88 75 66 48
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 631 1350
Size: 631 719
EcoO109I rG'GnC_Cy
Cuts at: 0 32 1037 1038 1350
Size: 32 1005 1 312
Fragments arranged by size:
1005 312 32 1
FspI TGC'GCA
Cuts at: 0 690 1007 1350
Size: 690 317 343
Fragments arranged by size:
690 343 317
HaeII r_GCGC'y
Cuts at: 0 836 1350
Size: 836 514
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 1011 1043 1134 1166 1198 1230
Size: 21 32 958 32 91 32 32 32
Cuts at: 1230 1350
Size: 120
Fragments arranged by size:
958 120 91 32 32 32 32 32
21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 846 1350
Size: 846 504
MslI CAynn'nnrTG
Cuts at: 0 539 1350
Size: 539 811
MspA1I CmG'CkG
Cuts at: 0 493 694 1011 1053 1350
Size: 493 201 317 42 297
Fragments arranged by size:
493 317 297 201 42
NspI r_CATG'y
Cuts at: 0 295 765 1350
Size: 295 470 585
Fragments arranged by size:
585 470 295
PflMI CCAn_nnn'nTGG
Cuts at: 0 1089 1326 1350
Size: 1089 237 24
Fragments arranged by size:
1089 237 24
PfoI T'CCnGG_A
Cuts at: 0 25 1106 1350
Size: 25 1081 244
Fragments arranged by size:
1081 244 25
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 802 834 1350
Size: 802 32 516
Fragments arranged by size:
802 516 32
PpuMI rG'GwC_Cy
Cuts at: 0 32 1350
Size: 32 1318
PspOMI G'GGCC_C
Cuts at: 0 1037 1350
Size: 1037 313
PstI C_TGCA'G
Cuts at: 0 470 686 845 1350
Size: 470 216 159 505
Fragments arranged by size:
505 470 216 159
PvuII CAG'CTG
Cuts at: 0 493 694 1011 1053 1350
Size: 493 201 317 42 297
Fragments arranged by size:
493 317 297 201 42
SapI GCTCTTCn'nnn_
Cuts at: 0 536 823 1350
Size: 536 287 527
Fragments arranged by size:
536 527 287
SexAI A'CCwGG_T
Cuts at: 0 1058 1350
Size: 1058 292
SfcI C'TryA_G
Cuts at: 0 387 466 682 841 1350
Size: 387 79 216 159 509
Fragments arranged by size:
509 387 216 159 79
SmaI CCC'GGG
Cuts at: 0 732 1350
Size: 732 618
SmlI C'TyrA_G
Cuts at: 0 508 1276 1350
Size: 508 768 74
Fragments arranged by size:
768 508 74
SphI G_CATG'C
Cuts at: 0 295 1350
Size: 295 1055
StuI AGG'CCT
Cuts at: 0 814 1350
Size: 814 536
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1003 1090 1350
Size: 1003 87 260
Fragments arranged by size:
1003 260 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 111 143 689 721 1350
Size: 111 32 546 32 629
Fragments arranged by size:
629 546 111 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 1257 1350
Size: 315 942 93
Fragments arranged by size:
942 315 93
XmaI C'CCGG_G
Cuts at: 0 730 1350
Size: 730 620
Enzymes that do cut:
AccI AcuI AhdI AleI AloI AlwNI ApaI ApoI
AvaI BanII BbvCI BcgI BclI BglII Bme1580I BpmI
Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BsrBI BsrDI BstAPI BstEII BstYI BtgI
BtgZI BtsI ClaI EarI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I MmeI MslI MspA1I NspI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SapI SexAI SfcI
SmaI SmlI SphI StuI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BciVI BfrBI BglI BlpI BmgBI
BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrFI BsrGI BssHII
BssSI BstBI BstXI BstZ17I Bsu36I CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StyI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI