Restriction map for
pF1KE0559
(Linear) (Six-base) MAPSORT of: KIEE0559.seq Check: 4170 from: 1 to: 885
KIEE0559.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 332 885
Size: 332 553
Acc65I G'GTAC_C
Cuts at: 0 515 885
Size: 515 370
AfeI AGC'GCT
Cuts at: 0 823 885
Size: 823 62
AflIII A'CryG_T
Cuts at: 0 677 885
Size: 677 208
AleI CACnn'nnGTG
Cuts at: 0 157 885
Size: 157 728
AlwNI CAG_nnn'CTG
Cuts at: 0 171 351 885
Size: 171 180 534
Fragments arranged by size:
534 180 171
ApaI G_GGCC'C
Cuts at: 0 89 885
Size: 89 796
BanI G'GyrC_C
Cuts at: 0 148 234 515 885
Size: 148 86 281 370
Fragments arranged by size:
370 281 148 86
BanII G_rGCy'C
Cuts at: 0 89 219 740 885
Size: 89 130 521 145
Fragments arranged by size:
521 145 130 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 587 799 885
Size: 587 212 86
Fragments arranged by size:
587 212 86
BbvCI CC'TCA_GC
Cuts at: 0 865 885
Size: 865 20
BglI GCCn_nnn'nGGC
Cuts at: 0 306 885
Size: 306 579
BlpI GC'TnA_GC
Cuts at: 0 544 885
Size: 544 341
Bme1580I G_kGCm'C
Cuts at: 0 89 239 885
Size: 89 150 646
Fragments arranged by size:
646 150 89
BmgBI CAC'GTC
Cuts at: 0 402 680 885
Size: 402 278 205
Fragments arranged by size:
402 278 205
BmrI ACTGGGnnnn_n'
Cuts at: 0 91 885
Size: 91 794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 298 512 643 885
Size: 55 243 214 131 242
Fragments arranged by size:
243 242 214 131 55
Bpu10I CC'TnA_GC
Cuts at: 0 449 865 885
Size: 449 416 20
Fragments arranged by size:
449 416 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 885
Size: 265 620
BsaI GGTCTCn'nnnn_
Cuts at: 0 414 646 885
Size: 414 232 239
Fragments arranged by size:
414 239 232
BsaAI yAC'GTr
Cuts at: 0 42 228 525 885
Size: 42 186 297 360
Fragments arranged by size:
360 297 186 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 739 769 885
Size: 739 30 116
Fragments arranged by size:
739 116 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 885
Size: 664 221
BseYI C'CCAG_C
Cuts at: 0 781 885
Size: 781 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 885
Size: 547 338
BsiHKAI G_wGCw'C
Cuts at: 0 740 885
Size: 740 145
BsiWI C'GTAC_G
Cuts at: 0 228 885
Size: 228 657
Bsp1286I G_dGCh'C
Cuts at: 0 89 219 239 740 885
Size: 89 130 20 501 145
Fragments arranged by size:
501 145 130 89 20
BspHI T'CATG_A
Cuts at: 0 764 885
Size: 764 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 332 885
Size: 128 204 553
Fragments arranged by size:
553 204 128
BsrBI CCG'CTC
Cuts at: 0 57 875 885
Size: 57 818 10
Fragments arranged by size:
818 57 10
BstYI r'GATC_y
Cuts at: 0 617 885
Size: 617 268
Bsu36I CC'TnA_GG
Cuts at: 0 270 885
Size: 270 615
BtgI C'CryG_G
Cuts at: 0 304 656 806 885
Size: 304 352 150 79
Fragments arranged by size:
352 304 150 79
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 383 418 885
Size: 383 35 467
Fragments arranged by size:
467 383 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 406 885
Size: 406 479
EarI CTCTTCn'nnn_
Cuts at: 0 577 885
Size: 577 308
EcoICRI GAG'CTC
Cuts at: 0 738 885
Size: 738 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 298 512 643 885
Size: 55 243 214 131 242
Fragments arranged by size:
243 242 214 131 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 765 885
Size: 646 119 120
Fragments arranged by size:
646 120 119
EcoO109I rG'GnC_Cy
Cuts at: 0 51 85 86 458 509 885
Size: 51 34 1 372 51 376
Fragments arranged by size:
376 372 51 51 34 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 816 885
Size: 816 69
HaeII r_GCGC'y
Cuts at: 0 639 825 885
Size: 639 186 60
Fragments arranged by size:
639 186 60
KpnI G_GTAC'C
Cuts at: 0 519 885
Size: 519 366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 885
Size: 335 550
MslI CAynn'nnrTG
Cuts at: 0 157 885
Size: 157 728
NcoI C'CATG_G
Cuts at: 0 656 885
Size: 656 229
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 432 885
Size: 171 261 453
Fragments arranged by size:
453 261 171
PfoI T'CCnGG_A
Cuts at: 0 11 473 620 885
Size: 11 462 147 265
Fragments arranged by size:
462 265 147 11
PmlI CAC'GTG
Cuts at: 0 525 885
Size: 525 360
PpuMI rG'GwC_Cy
Cuts at: 0 51 458 509 885
Size: 51 407 51 376
Fragments arranged by size:
407 376 51 51
PspOMI G'GGCC_C
Cuts at: 0 85 885
Size: 85 800
PstI C_TGCA'G
Cuts at: 0 95 862 885
Size: 95 767 23
Fragments arranged by size:
767 95 23
SacI G_AGCT'C
Cuts at: 0 740 885
Size: 740 145
SanDI GG'GwC_CC
Cuts at: 0 51 885
Size: 51 834
ScaI AGT'ACT
Cuts at: 0 67 885
Size: 67 818
SfcI C'TryA_G
Cuts at: 0 91 858 885
Size: 91 767 27
Fragments arranged by size:
767 91 27
SmlI C'TyrA_G
Cuts at: 0 244 885
Size: 244 641
SnaBI TAC'GTA
Cuts at: 0 228 885
Size: 228 657
StyI C'CwwG_G
Cuts at: 0 656 885
Size: 656 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 147 568 885
Size: 147 421 317
Fragments arranged by size:
421 317 147
TatI w'GTAC_w
Cuts at: 0 65 885
Size: 65 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 885
Size: 739 32 114
Fragments arranged by size:
739 114 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 663 804 885
Size: 663 141 81
Fragments arranged by size:
663 141 81
Enzymes that do cut:
AarI Acc65I AfeI AflIII AleI AlwNI ApaI BanI
BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI BstYI
Bsu36I BtgI CspCI DrdI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII KpnI MmeI MslI NcoI PflMI
PfoI PmlI PpuMI PspOMI PstI SacI SanDI ScaI
SfcI SmlI SnaBI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII
BmtI BplI BsaBI BsaHI BsaWI BsiEI BsmI BsmBI
BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI DraI DraIII
EaeI EagI EciI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI MfeI MluI MscI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI