Restriction map for
pF1KE0560
(Linear) (Six-base) MAPSORT of: KIEE0560.seq Check: 8716 from: 1 to: 1392
KIEE0560.orf
With 182 enzymes: SgfI *
August 18, 2008 14:48 ..
AccI GT'mk_AC
Cuts at: 0 758 1392
Size: 758 634
AflII C'TTAA_G
Cuts at: 0 39 439 1392
Size: 39 400 953
Fragments arranged by size:
953 400 39
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 823 855 1392
Size: 823 32 537
Fragments arranged by size:
823 537 32
AlwNI CAG_nnn'CTG
Cuts at: 0 633 945 966 1110 1392
Size: 633 312 21 144 282
Fragments arranged by size:
633 312 282 144 21
ApaI G_GGCC'C
Cuts at: 0 1062 1392
Size: 1062 330
ApoI r'AATT_y
Cuts at: 0 130 1194 1239 1392
Size: 130 1064 45 153
Fragments arranged by size:
1064 153 130 45
AvaI C'yCGr_G
Cuts at: 0 750 1392
Size: 750 642
BamHI G'GATC_C
Cuts at: 0 22 1392
Size: 22 1370
BanI G'GyrC_C
Cuts at: 0 190 1392
Size: 190 1202
BanII G_rGCy'C
Cuts at: 0 318 548 1062 1392
Size: 318 230 514 330
Fragments arranged by size:
514 330 318 230
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 787 821 1392
Size: 787 34 571
Fragments arranged by size:
787 571 34
BclI T'GATC_A
Cuts at: 0 399 1392
Size: 399 993
BglI GCCn_nnn'nGGC
Cuts at: 0 508 1392
Size: 508 884
BglII A'GATC_T
Cuts at: 0 872 1392
Size: 872 520
Bme1580I G_kGCm'C
Cuts at: 0 195 1062 1392
Size: 195 867 330
Fragments arranged by size:
867 330 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 340 967 1009 1084 1132 1392
Size: 68 272 627 42 75 48 260
Fragments arranged by size:
627 272 260 75 68 48 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 1000 1087 1156 1392
Size: 1000 87 69 236
Fragments arranged by size:
1000 236 87 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 823 853 1392
Size: 823 30 539
Fragments arranged by size:
823 539 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 337 447 670 703 1081 1117 1392
Size: 232 105 110 223 33 378 36 275
Fragments arranged by size:
378 275 232 223 110 105 36 33
BseYI C'CCAG_C
Cuts at: 0 715 1392
Size: 715 677
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 1060 1392
Size: 101 959 332
Fragments arranged by size:
959 332 101
BsiHKAI G_wGCw'C
Cuts at: 0 318 548 997 1392
Size: 318 230 449 395
Fragments arranged by size:
449 395 318 230
Bsp1286I G_dGCh'C
Cuts at: 0 195 318 548 997 1062 1392
Size: 195 123 230 449 65 330
Fragments arranged by size:
449 330 230 195 123 65
BspHI T'CATG_A
Cuts at: 0 402 1392
Size: 402 990
BsrBI CCG'CTC
Cuts at: 0 790 1392
Size: 790 602
BstAPI GCAn_nnn'nTGC
Cuts at: 0 88 1392
Size: 88 1304
BstEII G'GTnAC_C
Cuts at: 0 261 1392
Size: 261 1131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 187 1392
Size: 187 1205
BstYI r'GATC_y
Cuts at: 0 22 55 382 435 872 950 1040 1392
Size: 22 33 327 53 437 78 90 352
Fragments arranged by size:
437 352 327 90 78 53 33 22
BtgI C'CryG_G
Cuts at: 0 390 1392
Size: 390 1002
BtsI GCAGTG_nn'
Cuts at: 0 105 168 712 1392
Size: 105 63 544 680
Fragments arranged by size:
680 544 105 63
EaeI y'GGCC_r
Cuts at: 0 971 1392
Size: 971 421
EarI CTCTTCn'nnn_
Cuts at: 0 680 844 1283 1392
Size: 680 164 439 109
Fragments arranged by size:
680 439 164 109
EcoICRI GAG'CTC
Cuts at: 0 316 546 1392
Size: 316 230 846
Fragments arranged by size:
846 316 230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 340 967 1009 1084 1132 1392
Size: 68 272 627 42 75 48 260
Fragments arranged by size:
627 272 260 75 68 48 42
EcoO109I rG'GnC_Cy
Cuts at: 0 1058 1059 1392
Size: 1058 1 333
Fragments arranged by size:
1058 333 1
EcoRI G'AATT_C
Cuts at: 0 1239 1392
Size: 1239 153
EcoRV GAT'ATC
Cuts at: 0 418 1392
Size: 418 974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1161 1392
Size: 1161 231
HaeII r_GCGC'y
Cuts at: 0 857 1392
Size: 857 535
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 651 683 684 716 1032 1064 1392
Size: 651 32 1 32 316 32 328
Fragments arranged by size:
651 328 316 32 32 32 1
MfeI C'AATT_G
Cuts at: 0 428 1392
Size: 428 964
MscI TGG'CCA
Cuts at: 0 973 1392
Size: 973 419
MslI CAynn'nnrTG
Cuts at: 0 185 922 1392
Size: 185 737 470
Fragments arranged by size:
737 470 185
MspA1I CmG'CkG
Cuts at: 0 957 1032 1074 1392
Size: 957 75 42 318
Fragments arranged by size:
957 318 75 42
NspI r_CATG'y
Cuts at: 0 34 107 786 1392
Size: 34 73 679 606
Fragments arranged by size:
679 606 73 34
PasI CC'CwG_GG
Cuts at: 0 1234 1392
Size: 1234 158
PflMI CCAn_nnn'nTGG
Cuts at: 0 1110 1392
Size: 1110 282
PfoI T'CCnGG_A
Cuts at: 0 596 1392
Size: 596 796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 1392
Size: 823 32 537
Fragments arranged by size:
823 537 32
PspOMI G'GGCC_C
Cuts at: 0 1058 1392
Size: 1058 334
PstI C_TGCA'G
Cuts at: 0 491 619 707 866 941 962 1392
Size: 491 128 88 159 75 21 430
Fragments arranged by size:
491 430 159 128 88 75 21
PvuII CAG'CTG
Cuts at: 0 957 1032 1074 1392
Size: 957 75 42 318
Fragments arranged by size:
957 318 75 42
SacI G_AGCT'C
Cuts at: 0 318 548 1392
Size: 318 230 844
Fragments arranged by size:
844 318 230
SapI GCTCTTCn'nnn_
Cuts at: 0 844 1283 1392
Size: 844 439 109
Fragments arranged by size:
844 439 109
SexAI A'CCwGG_T
Cuts at: 0 1079 1392
Size: 1079 313
SfcI C'TryA_G
Cuts at: 0 408 487 615 703 862 937 958 1392
Size: 408 79 128 88 159 75 21 434
Fragments arranged by size:
434 408 159 128 88 79 75 21
SmaI CCC'GGG
Cuts at: 0 752 1392
Size: 752 640
SmlI C'TyrA_G
Cuts at: 0 39 439 1392
Size: 39 400 953
Fragments arranged by size:
953 400 39
StuI AGG'CCT
Cuts at: 0 835 1392
Size: 835 557
StyI C'CwwG_G
Cuts at: 0 143 195 1392
Size: 143 52 1197
Fragments arranged by size:
1197 143 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 637 1024 1111 1392
Size: 637 387 87 281
Fragments arranged by size:
637 387 281 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 16 1266 1392
Size: 16 1250 126
Fragments arranged by size:
1250 126 16
XmaI C'CCGG_G
Cuts at: 0 750 1392
Size: 750 642
Enzymes that do cut:
AccI AflII AloI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BcgI BclI BglI BglII Bme1580I BpmI
BsaI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BsrBI BstAPI BstEII BstXI BstYI BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I MfeI MscI MslI MspA1I NspI PasI PflMI
PfoI PpiI PspOMI PstI PvuII SacI SapI SexAI
SfcI SmaI SmlI StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BciVI BfrBI BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI XhoI XmnI ZraI