Restriction map for
pF1KE0563
(Linear) (Six-base) MAPSORT of: KIEE0563.seq Check: 3270 from: 1 to: 666
KIEE0563.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 666
Size: 562 104
ApoI r'AATT_y
Cuts at: 0 97 666
Size: 97 569
AvaI C'yCGr_G
Cuts at: 0 513 666
Size: 513 153
BanII G_rGCy'C
Cuts at: 0 270 666
Size: 270 396
BlpI GC'TnA_GC
Cuts at: 0 457 666
Size: 457 209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 528 632 666
Size: 528 104 34
Fragments arranged by size:
528 104 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 510 666
Size: 98 412 156
Fragments arranged by size:
412 156 98
BsaAI yAC'GTr
Cuts at: 0 498 666
Size: 498 168
BseYI C'CCAG_C
Cuts at: 0 461 666
Size: 461 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 666
Size: 217 449
Bsp1286I G_dGCh'C
Cuts at: 0 270 666
Size: 270 396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 578 666
Size: 578 88
BsrDI GCAATG_nn'
Cuts at: 0 221 666
Size: 221 445
BsrGI T'GTAC_A
Cuts at: 0 281 666
Size: 281 385
BtgI C'CryG_G
Cuts at: 0 575 620 666
Size: 575 45 46
Fragments arranged by size:
575 46 45
DraIII CAC_nnn'GTG
Cuts at: 0 480 666
Size: 480 186
EarI CTCTTCn'nnn_
Cuts at: 0 86 497 666
Size: 86 411 169
Fragments arranged by size:
411 169 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 510 562 666
Size: 98 412 52 104
Fragments arranged by size:
412 104 98 52
EcoO109I rG'GnC_Cy
Cuts at: 0 546 666
Size: 546 120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 381 457 666
Size: 381 76 209
Fragments arranged by size:
381 209 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 648 666
Size: 616 32 18
Fragments arranged by size:
616 32 18
HincII GTy'rAC
Cuts at: 0 337 666
Size: 337 329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 666
Size: 68 598
MspA1I CmG'CkG
Cuts at: 0 76 666
Size: 76 590
NcoI C'CATG_G
Cuts at: 0 620 666
Size: 620 46
NdeI CA'TA_TG
Cuts at: 0 331 666
Size: 331 335
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 666
Size: 59 607
PshAI GACnn'nnGTC
Cuts at: 0 515 666
Size: 515 151
PspXI vC'TCGA_Gb
Cuts at: 0 513 666
Size: 513 153
PstI C_TGCA'G
Cuts at: 0 75 114 596 666
Size: 75 39 482 70
Fragments arranged by size:
482 75 70 39
PvuII CAG'CTG
Cuts at: 0 76 666
Size: 76 590
SapI GCTCTTCn'nnn_
Cuts at: 0 497 666
Size: 497 169
SexAI A'CCwGG_T
Cuts at: 0 476 666
Size: 476 190
SfcI C'TryA_G
Cuts at: 0 71 110 592 666
Size: 71 39 482 74
Fragments arranged by size:
482 74 71 39
SmlI C'TyrA_G
Cuts at: 0 513 666
Size: 513 153
StyI C'CwwG_G
Cuts at: 0 365 620 666
Size: 365 255 46
Fragments arranged by size:
365 255 46
TatI w'GTAC_w
Cuts at: 0 281 666
Size: 281 385
XbaI T'CTAG_A
Cuts at: 0 102 666
Size: 102 564
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 363 666
Size: 363 303
XhoI C'TCGA_G
Cuts at: 0 513 666
Size: 513 153
XmnI GAAnn'nnTTC
Cuts at: 0 655 666
Size: 655 11
Enzymes that do cut:
AcuI ApoI AvaI BanII BlpI BplI BpmI BsaAI
BseYI BsgI Bsp1286I BspMI BsrDI BsrGI BtgI DraIII
EarI Eco57MI EcoO109I FalI Hin4I HincII MmeI MspA1I
NcoI NdeI PflMI PshAI PspXI PstI PvuII SapI
SexAI SfcI SmlI StyI TatI XbaI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XmaI ZraI