(Linear) (Six-base) MAPSORT of: KIEE0565.seq  Check: 2664  from: 1  to: 714



KIEE0565.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     272     449     479     509     566     596     653     683 
   Size:        272     177      30      30      57      30      57      30

Cuts at:    683     714 
   Size:         31

  Fragments arranged by size: 

                 272     177      57      57      31      30      30      30
                  30
 
BanII G_rGCy'C

Cuts at:      0     187     349     714 
   Size:        187     162     365

  Fragments arranged by size: 

                 365     187     162
 
Bme1580I G_kGCm'C

Cuts at:      0     281     714 
   Size:        281     433
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     375     462     492     579     666     714 
   Size:        375      87      30      87      87      48

  Fragments arranged by size: 

                 375      87      87      87      48      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18      39      41     147     249     279     714 
   Size:         18      21       2     106     102      30     435

  Fragments arranged by size: 

                 435     106     102      30      21      18       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     282     402     519     606     657     714 
   Size:        282     120     117      87      51      57

  Fragments arranged by size: 

                 282     120     117      87      57      51
 
BseYI C'CCAG_C

Cuts at:      0     457     714 
   Size:        457     257
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     714 
   Size:        405     309
 
Bsp1286I G_dGCh'C

Cuts at:      0     187     281     349     714 
   Size:        187      94      68     365

  Fragments arranged by size: 

                 365     187      94      68
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     575     662     714 
   Size:        575      87      52

  Fragments arranged by size: 

                 575      87      52
 
Bsu36I CC'TnA_GG

Cuts at:      0     414     531     618     714 
   Size:        414     117      87      96

  Fragments arranged by size: 

                 414     117      96      87
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18      39      41     147     249     279     714 
   Size:         18      21       2     106     102      30     435

  Fragments arranged by size: 

                 435     106     102      30      21      18       2
 
MspA1I CmG'CkG

Cuts at:      0      38      59     167     269     299     389     446     461 
   Size:         38      21     108     102      30      90      57      15

Cuts at:    461     476     506     563     593     650     680     714 
   Size:         15      30      57      30      57      30      34

  Fragments arranged by size: 

                 108     102      90      57      57      57      38      34
                  30      30      30      30      21      15      15
 
PvuII CAG'CTG

Cuts at:      0      38      59     167     269     299     389     446     461 
   Size:         38      21     108     102      30      90      57      15

Cuts at:    461     476     506     563     593     650     680     714 
   Size:         15      30      57      30      57      30      34

  Fragments arranged by size: 

                 108     102      90      57      57      57      38      34
                  30      30      30      30      21      15      15
 
StyI C'CwwG_G

Cuts at:      0     188     248     320     350     714 
   Size:        188      60      72      30     364

  Fragments arranged by size: 

                 364     188      72      60      30
 

 Enzymes that do cut:

    AlwNI    BanII Bme1580I     BmrI     BpmI    BseRI    BseYI     BsgI
 Bsp1286I   BstAPI   Bsu36I  Eco57MI   MspA1I    PvuII     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI