(Linear) (Six-base) MAPSORT of: KIEE0574.seq  Check: 6503  from: 1  to: 870



KIEE0574.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     439     870 
   Size:        439     431
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     599     829     835     870 
   Size:        106     493     230       6      35

  Fragments arranged by size: 

                 493     230     106      35       6
 
AleI CACnn'nnGTG

Cuts at:      0     711     870 
   Size:        711     159
 
AlwNI CAG_nnn'CTG

Cuts at:      0     146     870 
   Size:        146     724
 
ApoI r'AATT_y

Cuts at:      0     403     505     638     870 
   Size:        403     102     133     232

  Fragments arranged by size: 

                 403     232     133     102
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     835     870 
   Size:        835      35
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     735     870 
   Size:        735     135
 
BsaHI Gr'CG_yC

Cuts at:      0     323     870 
   Size:        323     547
 
BsaWI w'CCGG_w

Cuts at:      0      47     446     870 
   Size:         47     399     424

  Fragments arranged by size: 

                 424     399      47
 
BspEI T'CCGG_A

Cuts at:      0     446     870 
   Size:        446     424
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     439     870 
   Size:        439     431
 
BsrDI GCAATG_nn'

Cuts at:      0     837     870 
   Size:        837      33
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     256     870 
   Size:        256     614
 
BstBI TT'CG_AA

Cuts at:      0     783     870 
   Size:        783      87
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     622     870 
   Size:        622     248
 
BtgI C'CryG_G

Cuts at:      0      37     759     870 
   Size:         37     722     111

  Fragments arranged by size: 

                 722     111      37
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     481     870 
   Size:        481     389
 
EaeI y'GGCC_r

Cuts at:      0     189     345     612     870 
   Size:        189     156     267     258

  Fragments arranged by size: 

                 267     258     189     156
 
EarI CTCTTCn'nnn_

Cuts at:      0     279     506     870 
   Size:        279     227     364

  Fragments arranged by size: 

                 364     279     227
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     599     829     835     870 
   Size:        106     493     230       6      35

  Fragments arranged by size: 

                 493     230     106      35       6
 
HincII GTy'rAC

Cuts at:      0     291     870 
   Size:        291     579
 
MscI TGG'CCA

Cuts at:      0     191     347     614     870 
   Size:        191     156     267     256

  Fragments arranged by size: 

                 267     256     191     156
 
MslI CAynn'nnrTG

Cuts at:      0     707     711     870 
   Size:        707       4     159

  Fragments arranged by size: 

                 707     159       4
 
NcoI C'CATG_G

Cuts at:      0     759     870 
   Size:        759     111
 
StyI C'CwwG_G

Cuts at:      0     621     759     870 
   Size:        621     138     111

  Fragments arranged by size: 

                 621     138     111
 
TatI w'GTAC_w

Cuts at:      0     696     870 
   Size:        696     174
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      29      61     870 
   Size:         29      32     809

  Fragments arranged by size: 

                 809      32      29
 
XbaI T'CTAG_A

Cuts at:      0     399     870 
   Size:        399     471
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApoI     BbsI     BplI    BsaHI
    BsaWI    BspEI    BspMI    BsrDI   BstAPI    BstBI    BstXI     BtgI
    BtgZI     EaeI     EarI  Eco57MI   HincII     MscI     MslI     NcoI
     StyI     TatI     TstI     XbaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstEII    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI