Restriction map for
pF1KE0575
(Linear) (Six-base) MAPSORT of: KIEE0575.seq Check: 2913 from: 1 to: 1056
KIEE0575.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1056
Size: 352 704
AflIII A'CryG_T
Cuts at: 0 129 1056
Size: 129 927
AleI CACnn'nnGTG
Cuts at: 0 685 1056
Size: 685 371
AlwNI CAG_nnn'CTG
Cuts at: 0 584 865 1056
Size: 584 281 191
Fragments arranged by size:
584 281 191
ApoI r'AATT_y
Cuts at: 0 287 448 1056
Size: 287 161 608
Fragments arranged by size:
608 287 161
AvrII C'CTAG_G
Cuts at: 0 120 1056
Size: 120 936
BamHI G'GATC_C
Cuts at: 0 515 1056
Size: 515 541
BanII G_rGCy'C
Cuts at: 0 109 1056
Size: 109 947
BclI T'GATC_A
Cuts at: 0 647 1056
Size: 647 409
BglII A'GATC_T
Cuts at: 0 53 1056
Size: 53 1003
Bme1580I G_kGCm'C
Cuts at: 0 473 1056
Size: 473 583
BmgBI CAC'GTC
Cuts at: 0 130 1056
Size: 130 926
BmrI ACTGGGnnnn_n'
Cuts at: 0 200 620 913 1056
Size: 200 420 293 143
Fragments arranged by size:
420 293 200 143
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 342 982 1056
Size: 342 640 74
Fragments arranged by size:
640 342 74
Bpu10I CC'TnA_GC
Cuts at: 0 74 1056
Size: 74 982
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1056
Size: 997 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 116 146 370 395 400 425
Size: 70 30 16 30 224 25 5 25
Cuts at: 425 1056
Size: 631
Fragments arranged by size:
631 224 70 30 30 25 25 16
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 955 1056
Size: 955 101
BseYI C'CCAG_C
Cuts at: 0 485 511 705 1056
Size: 485 26 194 351
Fragments arranged by size:
485 351 194 26
BsiHKAI G_wGCw'C
Cuts at: 0 75 1056
Size: 75 981
BsmI GAATG_Cn'
Cuts at: 0 480 823 1056
Size: 480 343 233
Fragments arranged by size:
480 343 233
Bsp1286I G_dGCh'C
Cuts at: 0 75 109 473 1056
Size: 75 34 364 583
Fragments arranged by size:
583 364 75 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1039 1056
Size: 1039 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 190 687 1056
Size: 190 497 369
Fragments arranged by size:
497 369 190
BstYI r'GATC_y
Cuts at: 0 53 515 1056
Size: 53 462 541
Fragments arranged by size:
541 462 53
BtgI C'CryG_G
Cuts at: 0 542 1056
Size: 542 514
EaeI y'GGCC_r
Cuts at: 0 100 1056
Size: 100 956
EarI CTCTTCn'nnn_
Cuts at: 0 730 1056
Size: 730 326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1056
Size: 352 704
EcoO109I rG'GnC_Cy
Cuts at: 0 194 269 547 872 1056
Size: 194 75 278 325 184
Fragments arranged by size:
325 278 194 184 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 779 811 1056
Size: 70 32 677 32 245
Fragments arranged by size:
677 245 70 32 32
HincII GTy'rAC
Cuts at: 0 719 1056
Size: 719 337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 264 1056
Size: 251 13 792
Fragments arranged by size:
792 251 13
MslI CAynn'nnrTG
Cuts at: 0 68 685 1056
Size: 68 617 371
Fragments arranged by size:
617 371 68
MspA1I CmG'CkG
Cuts at: 0 316 929 1056
Size: 316 613 127
Fragments arranged by size:
613 316 127
NcoI C'CATG_G
Cuts at: 0 542 1056
Size: 542 514
NdeI CA'TA_TG
Cuts at: 0 169 1036 1056
Size: 169 867 20
Fragments arranged by size:
867 169 20
NspI r_CATG'y
Cuts at: 0 67 400 1056
Size: 67 333 656
Fragments arranged by size:
656 333 67
PflMI CCAn_nnn'nTGG
Cuts at: 0 504 865 1056
Size: 504 361 191
Fragments arranged by size:
504 361 191
PpuMI rG'GwC_Cy
Cuts at: 0 194 269 547 872 1056
Size: 194 75 278 325 184
Fragments arranged by size:
325 278 194 184 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 748 780 1056
Size: 748 32 276
Fragments arranged by size:
748 276 32
PstI C_TGCA'G
Cuts at: 0 733 1056
Size: 733 323
PvuII CAG'CTG
Cuts at: 0 316 1056
Size: 316 740
SanDI GG'GwC_CC
Cuts at: 0 194 1056
Size: 194 862
ScaI AGT'ACT
Cuts at: 0 962 1056
Size: 962 94
SfcI C'TryA_G
Cuts at: 0 273 576 729 876 1056
Size: 273 303 153 147 180
Fragments arranged by size:
303 273 180 153 147
SmlI C'TyrA_G
Cuts at: 0 1012 1056
Size: 1012 44
SphI G_CATG'C
Cuts at: 0 400 1056
Size: 400 656
SspI AAT'ATT
Cuts at: 0 179 1056
Size: 179 877
StyI C'CwwG_G
Cuts at: 0 120 542 1056
Size: 120 422 514
Fragments arranged by size:
514 422 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 395 697 1056
Size: 395 302 359
Fragments arranged by size:
395 359 302
TatI w'GTAC_w
Cuts at: 0 960 1056
Size: 960 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 370 395 402 427 1056
Size: 116 32 222 25 7 25 629
Fragments arranged by size:
629 222 116 32 25 25 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 633 702 840 1056
Size: 633 69 138 216
Fragments arranged by size:
633 216 138 69
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI AvrII BamHI BanII
BclI BglII Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BstXI
BstYI BtgI EaeI EarI Eco57MI EcoO109I Hin4I HincII
MmeI MslI MspA1I NcoI NdeI NspI PflMI PpuMI
PsrI PstI PvuII SanDI ScaI SfcI SmlI SphI
SspI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BlpI BmtI BpmI BsaI BsaAI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI