Restriction map for
pF1KE0579
(Linear) (Six-base) MAPSORT of: KIEE0579.seq Check: 1679 from: 1 to: 837
KIEE0579.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 837
Size: 686 151
AleI CACnn'nnGTG
Cuts at: 0 55 837
Size: 55 782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 837
Size: 31 806
AlwNI CAG_nnn'CTG
Cuts at: 0 382 837
Size: 382 455
ApoI r'AATT_y
Cuts at: 0 148 837
Size: 148 689
BanI G'GyrC_C
Cuts at: 0 62 837
Size: 62 775
BanII G_rGCy'C
Cuts at: 0 429 837
Size: 429 408
BbeI G_GCGC'C
Cuts at: 0 66 837
Size: 66 771
BbsI GAAGACnn'nnnn_
Cuts at: 0 225 568 723 837
Size: 225 343 155 114
Fragments arranged by size:
343 225 155 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 500 534 837
Size: 500 34 303
Fragments arranged by size:
500 303 34
BlpI GC'TnA_GC
Cuts at: 0 416 795 837
Size: 416 379 42
Fragments arranged by size:
416 379 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 739 837
Size: 315 424 98
Fragments arranged by size:
424 315 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 460 837
Size: 143 317 377
Fragments arranged by size:
377 317 143
BsaI GGTCTCn'nnnn_
Cuts at: 0 331 837
Size: 331 506
BsaAI yAC'GTr
Cuts at: 0 294 837
Size: 294 543
BsaHI Gr'CG_yC
Cuts at: 0 63 837
Size: 63 774
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 837
Size: 196 30 611
Fragments arranged by size:
611 196 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 346 349 837
Size: 49 297 3 488
Fragments arranged by size:
488 297 49 3
BseYI C'CCAG_C
Cuts at: 0 164 259 420 490 837
Size: 164 95 161 70 347
Fragments arranged by size:
347 164 161 95 70
BsiHKAI G_wGCw'C
Cuts at: 0 83 712 837
Size: 83 629 125
Fragments arranged by size:
629 125 83
Bsp1286I G_dGCh'C
Cuts at: 0 83 429 712 837
Size: 83 346 283 125
Fragments arranged by size:
346 283 125 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 544 837
Size: 544 293
BsrFI r'CCGG_y
Cuts at: 0 41 837
Size: 41 796
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 475 837
Size: 57 418 362
Fragments arranged by size:
418 362 57
Bsu36I CC'TnA_GG
Cuts at: 0 391 837
Size: 391 446
BtgI C'CryG_G
Cuts at: 0 56 837
Size: 56 781
EarI CTCTTCn'nnn_
Cuts at: 0 687 837
Size: 687 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 82 837
Size: 46 36 755
Fragments arranged by size:
755 46 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 460 686 837
Size: 143 317 226 151
Fragments arranged by size:
317 226 151 143
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 837
Size: 346 491
EcoO109I rG'GnC_Cy
Cuts at: 0 659 837
Size: 659 178
EcoRI G'AATT_C
Cuts at: 0 148 837
Size: 148 689
HaeII r_GCGC'y
Cuts at: 0 48 66 266 837
Size: 48 18 200 571
Fragments arranged by size:
571 200 48 18
HindIII A'AGCT_T
Cuts at: 0 792 837
Size: 792 45
KasI G'GCGC_C
Cuts at: 0 62 837
Size: 62 775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 801 837
Size: 801 36
MslI CAynn'nnrTG
Cuts at: 0 55 247 473 837
Size: 55 192 226 364
Fragments arranged by size:
364 226 192 55
MspA1I CmG'CkG
Cuts at: 0 219 420 596 837
Size: 219 201 176 241
Fragments arranged by size:
241 219 201 176
NaeI GCC'GGC
Cuts at: 0 43 837
Size: 43 794
NarI GG'CG_CC
Cuts at: 0 63 837
Size: 63 774
NgoMIV G'CCGG_C
Cuts at: 0 41 837
Size: 41 796
NspI r_CATG'y
Cuts at: 0 506 837
Size: 506 331
PasI CC'CwG_GG
Cuts at: 0 376 453 837
Size: 376 77 384
Fragments arranged by size:
384 376 77
PflMI CCAn_nnn'nTGG
Cuts at: 0 382 837
Size: 382 455
PfoI T'CCnGG_A
Cuts at: 0 437 783 837
Size: 437 346 54
Fragments arranged by size:
437 346 54
PmlI CAC'GTG
Cuts at: 0 294 837
Size: 294 543
PpuMI rG'GwC_Cy
Cuts at: 0 659 837
Size: 659 178
PvuII CAG'CTG
Cuts at: 0 420 837
Size: 420 417
SfoI GGC'GCC
Cuts at: 0 64 837
Size: 64 773
StyI C'CwwG_G
Cuts at: 0 522 837
Size: 522 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 837
Size: 178 659
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 661 693 837
Size: 196 32 433 32 144
Fragments arranged by size:
433 196 144 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 779 837
Size: 779 58
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApoI BanI BanII BbeI
BbsI BcgI BlpI BmrI BpmI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrFI BstXI
Bsu36I BtgI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
HaeII HindIII KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PvuII
SfoI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI
BglI BglII Bme1580I BmgBI BmtI BplI Bpu10I BpuEI
BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoICRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI ZraI