(Linear) (Six-base) MAPSORT of: KIEE0582.seq  Check: 5703  from: 1  to: 918



KIEE0582.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     695     918 
   Size:        695     223
 
ApoI r'AATT_y

Cuts at:      0      16      58     431     918 
   Size:         16      42     373     487

  Fragments arranged by size: 

                 487     373      42      16
 
BclI T'GATC_A

Cuts at:      0      92     918 
   Size:         92     826
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     471     918 
   Size:        471     447
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     918 
   Size:        559     359
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     310     668     918 
   Size:        310     358     250

  Fragments arranged by size: 

                 358     310     250
 
BseYI C'CCAG_C

Cuts at:      0      27     141     918 
   Size:         27     114     777

  Fragments arranged by size: 

                 777     114      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     534     918 
   Size:        534     384
 
BsiHKAI G_wGCw'C

Cuts at:      0     655     918 
   Size:        655     263
 
Bsp1286I G_dGCh'C

Cuts at:      0     655     918 
   Size:        655     263
 
BspHI T'CATG_A

Cuts at:      0     389     918 
   Size:        389     529
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      94     119     918 
   Size:         94      25     799

  Fragments arranged by size: 

                 799      94      25
 
BstEII G'GTnAC_C

Cuts at:      0     106     918 
   Size:        106     812
 
Bsu36I CC'TnA_GG

Cuts at:      0     658     918 
   Size:        658     260
 
BtsI GCAGTG_nn'

Cuts at:      0     869     918 
   Size:        869      49
 
EaeI y'GGCC_r

Cuts at:      0     356     918 
   Size:        356     562
 
EarI CTCTTCn'nnn_

Cuts at:      0     210     292     539     918 
   Size:        210      82     247     379

  Fragments arranged by size: 

                 379     247     210      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     695     918 
   Size:        559     136     223

  Fragments arranged by size: 

                 559     223     136
 
EcoO109I rG'GnC_Cy

Cuts at:      0     571     918 
   Size:        571     347
 
EcoRI G'AATT_C

Cuts at:      0      16     431     918 
   Size:         16     415     487

  Fragments arranged by size: 

                 487     415      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     226     258     918 
   Size:        226      32     660

  Fragments arranged by size: 

                 660     226      32
 
MscI TGG'CCA

Cuts at:      0     358     918 
   Size:        358     560
 
MspA1I CmG'CkG

Cuts at:      0     313     918 
   Size:        313     605
 
PasI CC'CwG_GG

Cuts at:      0     147     918 
   Size:        147     771
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     490     918 
   Size:        490     428
 
PstI C_TGCA'G

Cuts at:      0      56     918 
   Size:         56     862
 
PvuII CAG'CTG

Cuts at:      0     313     918 
   Size:        313     605
 
SapI GCTCTTCn'nnn_

Cuts at:      0     292     918 
   Size:        292     626
 
SfcI C'TryA_G

Cuts at:      0      52     918 
   Size:         52     866
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27      59     590     622     918 
   Size:         27      32     531      32     296

  Fragments arranged by size: 

                 531     296      32      32      27
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     403     918 
   Size:        403     515
 

 Enzymes that do cut:

     AcuI     ApoI     BclI     BmrI     BpmI     BsaI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI   BstEII   Bsu36I     BtsI     EaeI
     EarI  Eco57MI EcoO109I    EcoRI    Hin4I     MscI   MspA1I     PasI
    PflMI     PstI    PvuII     SapI     SfcI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI