(Linear) (Six-base) MAPSORT of: KIEE0586.seq  Check: 7256  from: 1  to: 495



KIEE0586.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     104     347     377     434     495 
   Size:        104     243      30      57      61

  Fragments arranged by size: 

                 243     104      61      57      30
 
BanII G_rGCy'C

Cuts at:      0     187     217     495 
   Size:        187      30     278

  Fragments arranged by size: 

                 278     187      30
 
Bme1580I G_kGCm'C

Cuts at:      0     113     495 
   Size:        113     382
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     273     447     495 
   Size:        273     174      48

  Fragments arranged by size: 

                 273     174      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     147     495 
   Size:         81      66     348

  Fragments arranged by size: 

                 348      81      66
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     300     495 
   Size:        300     195
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     303     495 
   Size:        303     192
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     187     217     495 
   Size:        113      74      30     278

  Fragments arranged by size: 

                 278     113      74      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     443     495 
   Size:        443      52
 
Bsu36I CC'TnA_GG

Cuts at:      0     312     399     495 
   Size:        312      87      96

  Fragments arranged by size: 

                 312      96      87
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     147     495 
   Size:         81      66     348

  Fragments arranged by size: 

                 348      81      66
 
MspA1I CmG'CkG

Cuts at:      0     101     167     287     344     374     495 
   Size:        101      66     120      57      30     121

  Fragments arranged by size: 

                 121     120     101      66      57      30
 
PvuII CAG'CTG

Cuts at:      0     101     167     287     344     374     495 
   Size:        101      66     120      57      30     121

  Fragments arranged by size: 

                 121     120     101      66      57      30
 
StyI C'CwwG_G

Cuts at:      0     188     218     495 
   Size:        188      30     277

  Fragments arranged by size: 

                 277     188      30
 

 Enzymes that do cut:

    AlwNI    BanII Bme1580I     BmrI     BpmI    BseRI     BsgI Bsp1286I
   BstAPI   Bsu36I  Eco57MI   MspA1I    PvuII     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI