(Linear) (Six-base) MAPSORT of: KIEE0587.seq  Check: 7414  from: 1  to: 834



KIEE0587.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     125     389     716     773     803     834 
   Size:        125     264     327      57      30      31

  Fragments arranged by size: 

                 327     264     125      57      31      30
 
BamHI G'GATC_C

Cuts at:      0     280     340     574     834 
   Size:        280      60     234     260

  Fragments arranged by size: 

                 280     260     234      60
 
BanII G_rGCy'C

Cuts at:      0     223     313     517     834 
   Size:        223      90     204     317

  Fragments arranged by size: 

                 317     223     204      90
 
Bme1580I G_kGCm'C

Cuts at:      0     134     632     834 
   Size:        134     498     202

  Fragments arranged by size: 

                 498     202     134
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     699     786     834 
   Size:        699      87      48

  Fragments arranged by size: 

                 699      87      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18     102     168     366     432     834 
   Size:         18      84      66     198      66     402

  Fragments arranged by size: 

                 402     198      84      66      66      18
 
BsaWI w'CCGG_w

Cuts at:      0      19     834 
   Size:         19     815
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     729     834 
   Size:        729     105
 
BsiHKAI G_wGCw'C

Cuts at:      0     487     834 
   Size:        487     347
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     223     313     487     517     632     834 
   Size:        134      89      90     174      30     115     202

  Fragments arranged by size: 

                 202     174     134     115      90      89      30
 
BspEI T'CCGG_A

Cuts at:      0      19     834 
   Size:         19     815
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     782     834 
   Size:        782      52
 
BstYI r'GATC_y

Cuts at:      0     280     340     574     834 
   Size:        280      60     234     260

  Fragments arranged by size: 

                 280     260     234      60
 
Bsu36I CC'TnA_GG

Cuts at:      0     738     834 
   Size:        738      96
 
EarI CTCTTCn'nnn_

Cuts at:      0     216     834 
   Size:        216     618
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18     102     168     366     432     834 
   Size:         18      84      66     198      66     402

  Fragments arranged by size: 

                 402     198      84      66      66      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     494     834 
   Size:        494     340
 
MspA1I CmG'CkG

Cuts at:      0      38     122     188     386     452     770     834 
   Size:         38      84      66     198      66     318      64

  Fragments arranged by size: 

                 318     198      84      66      66      64      38
 
PvuII CAG'CTG

Cuts at:      0      38     122     188     386     452     770     834 
   Size:         38      84      66     198      66     318      64

  Fragments arranged by size: 

                 318     198      84      66      66      64      38
 
SapI GCTCTTCn'nnn_

Cuts at:      0     216     834 
   Size:        216     618
 
StyI C'CwwG_G

Cuts at:      0     224     284     314     344     488     518     578     834 
   Size:        224      60      30      30     144      30      60     256

  Fragments arranged by size: 

                 256     224     144      60      60      30      30      30
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     470     834 
   Size:        470     364
 

 Enzymes that do cut:

    AlwNI    BamHI    BanII Bme1580I     BmrI     BpmI    BsaWI     BsgI
  BsiHKAI Bsp1286I    BspEI   BstAPI    BstYI   Bsu36I     EarI  Eco57MI
 EcoO109I   MspA1I    PvuII     SapI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI