Restriction map for
pF1KE0597
(Linear) (Six-base) MAPSORT of: KIEE0597.seq Check: 2882 from: 1 to: 4449
KIEE0597.orf
With 182 enzymes: SgfI *
June 11, 2012 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1176 4449
Size: 1176 3273
AatII G_ACGT'C
Cuts at: 0 2224 4449
Size: 2224 2225
Acc65I G'GTAC_C
Cuts at: 0 3071 4449
Size: 3071 1378
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1106 1303 1637 1817 2525 2633 3283 3758
Size: 1106 197 334 180 708 108 650 475
Cuts at: 3758 3979 4062 4074 4406 4449
Size: 221 83 12 332 43
Fragments arranged by size:
1106 708 650 475 334 332 221 197
180 108 83 43 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2333 2554 4288 4449
Size: 2333 221 1734 161
Fragments arranged by size:
2333 1734 221 161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2555 2587 4162 4194 4449
Size: 2555 32 1575 32 255
Fragments arranged by size:
2555 1575 255 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2503 2647 2839 4449
Size: 2503 144 192 1610
Fragments arranged by size:
2503 1610 192 144
ApoI r'AATT_y
Cuts at: 0 646 1071 1257 1365 1843 2206 3114 3235
Size: 646 425 186 108 478 363 908 121
Cuts at: 3235 3319 4449
Size: 84 1130
Fragments arranged by size:
1130 908 646 478 425 363 186 121
108 84
AseI AT'TA_AT
Cuts at: 0 569 4449
Size: 569 3880
AvaI C'yCGr_G
Cuts at: 0 101 3245 3912 4449
Size: 101 3144 667 537
Fragments arranged by size:
3144 667 537 101
AvrII C'CTAG_G
Cuts at: 0 1381 4449
Size: 1381 3068
BamHI G'GATC_C
Cuts at: 0 931 2264 4449
Size: 931 1333 2185
Fragments arranged by size:
2185 1333 931
BanI G'GyrC_C
Cuts at: 0 3 460 3071 3908 3954 4449
Size: 3 457 2611 837 46 495
Fragments arranged by size:
2611 837 495 457 46 3
BanII G_rGCy'C
Cuts at: 0 639 1359 1536 2122 2486 4012 4449
Size: 639 720 177 586 364 1526 437
Fragments arranged by size:
1526 720 639 586 437 364 177
BbeI G_GCGC'C
Cuts at: 0 7 3912 4449
Size: 7 3905 537
Fragments arranged by size:
3905 537 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1291 1504 2012 2513 3010 3463 4297 4443
Size: 1291 213 508 501 497 453 834 146
Cuts at: 4443 4449
Size: 6
Fragments arranged by size:
1291 834 508 501 497 453 213 146
6
BbvCI CC'TCA_GC
Cuts at: 0 3974 4244 4449
Size: 3974 270 205
Fragments arranged by size:
3974 270 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 569 603 4449
Size: 569 34 3846
Fragments arranged by size:
3846 569 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1782 1898 4449
Size: 1782 116 2551
Fragments arranged by size:
2551 1782 116
BclI T'GATC_A
Cuts at: 0 4136 4449
Size: 4136 313
BfrBI ATG'CAT
Cuts at: 0 781 4449
Size: 781 3668
BglI GCCn_nnn'nGGC
Cuts at: 0 3301 4449
Size: 3301 1148
BglII A'GATC_T
Cuts at: 0 2092 2240 4449
Size: 2092 148 2209
Fragments arranged by size:
2209 2092 148
BlpI GC'TnA_GC
Cuts at: 0 218 4276 4449
Size: 218 4058 173
Fragments arranged by size:
4058 218 173
Bme1580I G_kGCm'C
Cuts at: 0 3685 4449
Size: 3685 764
BmgBI CAC'GTC
Cuts at: 0 152 4449
Size: 152 4297
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1555 4449
Size: 1555 2894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 2215 3466 3471 4048 4449
Size: 480 1735 1251 5 577 401
Fragments arranged by size:
1735 1251 577 480 401 5
Bpu10I CC'TnA_GC
Cuts at: 0 509 3974 4244 4449
Size: 509 3465 270 205
Fragments arranged by size:
3465 509 270 205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1732 1869 1941 4449
Size: 373 1359 137 72 2508
Fragments arranged by size:
2508 1359 373 137 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 2191 2293 3840 3947 4449
Size: 2191 102 1547 107 502
Fragments arranged by size:
2191 1547 502 107 102
BsaAI yAC'GTr
Cuts at: 0 583 1017 3498 4449
Size: 583 434 2481 951
Fragments arranged by size:
2481 951 583 434
BsaHI Gr'CG_yC
Cuts at: 0 4 2221 3909 4449
Size: 4 2217 1688 540
Fragments arranged by size:
2217 1688 540 4
BsaWI w'CCGG_w
Cuts at: 0 2261 4449
Size: 2261 2188
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 274 304 549 579 1967 1997 4449
Size: 274 30 245 30 1388 30 2452
Fragments arranged by size:
2452 1388 274 245 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 247 629 1488 1669 2242 3799 3802
Size: 70 177 382 859 181 573 1557 3
Cuts at: 3802 3805 3817 3820 3823 4449
Size: 3 12 3 3 626
Fragments arranged by size:
1557 859 626 573 382 181 177 70
12 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 853 2780 3684 4449
Size: 853 1927 904 765
Fragments arranged by size:
1927 904 853 765
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 1946 3425 4186 4222 4449
Size: 1117 829 1479 761 36 227
Fragments arranged by size:
1479 1117 829 761 227 36
BsiHKAI G_wGCw'C
Cuts at: 0 582 1536 1592 4012 4449
Size: 582 954 56 2420 437
Fragments arranged by size:
2420 954 582 437 56
BsmI GAATG_Cn'
Cuts at: 0 1774 2055 4449
Size: 1774 281 2394
Fragments arranged by size:
2394 1774 281
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1543 4449
Size: 1543 2906
Bsp1286I G_dGCh'C
Cuts at: 0 582 639 1359 1536 1592 2122 2486 3685
Size: 582 57 720 177 56 530 364 1199
Cuts at: 3685 4012 4449
Size: 327 437
Fragments arranged by size:
1199 720 582 530 437 364 327 177
57 56
BspHI T'CATG_A
Cuts at: 0 329 2270 4449
Size: 329 1941 2179
Fragments arranged by size:
2179 1941 329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1176 4449
Size: 1176 3273
BsrBI CCG'CTC
Cuts at: 0 9 63 4196 4449
Size: 9 54 4133 253
Fragments arranged by size:
4133 253 54 9
BsrDI GCAATG_nn'
Cuts at: 0 1433 4449
Size: 1433 3016
BsrFI r'CCGG_y
Cuts at: 0 3645 3905 4449
Size: 3645 260 544
Fragments arranged by size:
3645 544 260
BssSI C'ACGA_G
Cuts at: 0 1352 4449
Size: 1352 3097
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3433 3501 3550 4449
Size: 3433 68 49 899
Fragments arranged by size:
3433 899 68 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1839 4449
Size: 1839 2610
BstYI r'GATC_y
Cuts at: 0 931 2092 2240 2264 4049 4449
Size: 931 1161 148 24 1785 400
Fragments arranged by size:
1785 1161 931 400 148 24
Bsu36I CC'TnA_GG
Cuts at: 0 3026 4449
Size: 3026 1423
BtgI C'CryG_G
Cuts at: 0 1360 1832 3720 4148 4260 4449
Size: 1360 472 1888 428 112 189
Fragments arranged by size:
1888 1360 472 428 189 112
BtsI GCAGTG_nn'
Cuts at: 0 2817 3685 4449
Size: 2817 868 764
Fragments arranged by size:
2817 868 764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3616 3651 3702 3737 4242 4277 4449
Size: 3616 35 51 35 505 35 172
Fragments arranged by size:
3616 505 172 51 35 35 35
DraI TTT'AAA
Cuts at: 0 3155 4449
Size: 3155 1294
DrdI GACnn_nn'nnGTC
Cuts at: 0 2833 4449
Size: 2833 1616
EaeI y'GGCC_r
Cuts at: 0 1328 1829 4335 4449
Size: 1328 501 2506 114
Fragments arranged by size:
2506 1328 501 114
EarI CTCTTCn'nnn_
Cuts at: 0 70 101 347 428 981 1689 2365 2521
Size: 70 31 246 81 553 708 676 156
Cuts at: 2521 2746 3061 3398 3734 3776 3794 4040 4449
Size: 225 315 337 336 42 18 246 409
Fragments arranged by size:
708 676 553 409 337 336 315 246
246 225 156 81 70 42 31 18
EcoICRI GAG'CTC
Cuts at: 0 1534 4010 4449
Size: 1534 2476 439
Fragments arranged by size:
2476 1534 439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1106 1303 1637 1817 2215 2525 2633
Size: 480 626 197 334 180 398 310 108
Cuts at: 2633 3283 3466 3471 3758 3979 4048 4062 4074
Size: 650 183 5 287 221 69 14 12
Cuts at: 4074 4406 4449
Size: 332 43
Fragments arranged by size:
650 626 480 398 334 332 310 287
221 197 183 180 108 69 43 14
12 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1157 4449
Size: 1157 3292
EcoO109I rG'GnC_Cy
Cuts at: 0 1385 4449
Size: 1385 3064
EcoRI G'AATT_C
Cuts at: 0 1071 1257 1365 1843 4449
Size: 1071 186 108 478 2606
Fragments arranged by size:
2606 1071 478 186 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1726 1956 3226 4092 4449
Size: 1726 230 1270 866 357
Fragments arranged by size:
1726 1270 866 357 230
FseI GG_CCGG'CC
Cuts at: 0 3649 4449
Size: 3649 800
FspI TGC'GCA
Cuts at: 0 2089 2695 4449
Size: 2089 606 1754
Fragments arranged by size:
2089 1754 606
HaeII r_GCGC'y
Cuts at: 0 7 738 1481 3912 4449
Size: 7 731 743 2431 537
Fragments arranged by size:
2431 743 731 537 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 460 468 492 2206 2238 2723 2755
Size: 436 24 8 24 1714 32 485 32
Cuts at: 2755 4449
Size: 1694
Fragments arranged by size:
1714 1694 485 436 32 32 24 24
8
HincII GTy'rAC
Cuts at: 0 1754 3046 4449
Size: 1754 1292 1403
Fragments arranged by size:
1754 1403 1292
HindIII A'AGCT_T
Cuts at: 0 256 2654 4449
Size: 256 2398 1795
Fragments arranged by size:
2398 1795 256
HpaI GTT'AAC
Cuts at: 0 1754 4449
Size: 1754 2695
KasI G'GCGC_C
Cuts at: 0 3 3908 4449
Size: 3 3905 541
Fragments arranged by size:
3905 541 3
KpnI G_GTAC'C
Cuts at: 0 3075 4449
Size: 3075 1374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1301 4398 4449
Size: 302 999 3097 51
Fragments arranged by size:
3097 999 302 51
MscI TGG'CCA
Cuts at: 0 1330 1831 4337 4449
Size: 1330 501 2506 112
Fragments arranged by size:
2506 1330 501 112
MslI CAynn'nnrTG
Cuts at: 0 662 1837 4343 4449
Size: 662 1175 2506 106
Fragments arranged by size:
2506 1175 662 106
MspA1I CmG'CkG
Cuts at: 0 33 2149 2644 4082 4449
Size: 33 2116 495 1438 367
Fragments arranged by size:
2116 1438 495 367 33
NaeI GCC'GGC
Cuts at: 0 3647 3907 4449
Size: 3647 260 542
Fragments arranged by size:
3647 542 260
NarI GG'CG_CC
Cuts at: 0 4 3909 4449
Size: 4 3905 540
Fragments arranged by size:
3905 540 4
NcoI C'CATG_G
Cuts at: 0 1832 4148 4449
Size: 1832 2316 301
Fragments arranged by size:
2316 1832 301
NdeI CA'TA_TG
Cuts at: 0 4344 4449
Size: 4344 105
NgoMIV G'CCGG_C
Cuts at: 0 3645 3905 4449
Size: 3645 260 544
Fragments arranged by size:
3645 544 260
NsiI A_TGCA'T
Cuts at: 0 783 4449
Size: 783 3666
PfoI T'CCnGG_A
Cuts at: 0 2573 2660 2757 3467 3487 4012 4449
Size: 2573 87 97 710 20 525 437
Fragments arranged by size:
2573 710 525 437 97 87 20
PmlI CAC'GTG
Cuts at: 0 3498 4449
Size: 3498 951
PpuMI rG'GwC_Cy
Cuts at: 0 1385 4449
Size: 1385 3064
PstI C_TGCA'G
Cuts at: 0 1422 2322 3894 4037 4449
Size: 1422 900 1572 143 412
Fragments arranged by size:
1572 1422 900 412 143
PvuII CAG'CTG
Cuts at: 0 2149 2644 4082 4449
Size: 2149 495 1438 367
Fragments arranged by size:
2149 1438 495 367
SacI G_AGCT'C
Cuts at: 0 1536 4012 4449
Size: 1536 2476 437
Fragments arranged by size:
2476 1536 437
SapI GCTCTTCn'nnn_
Cuts at: 0 70 2521 2746 4449
Size: 70 2451 225 1703
Fragments arranged by size:
2451 1703 225 70
ScaI AGT'ACT
Cuts at: 0 162 4449
Size: 162 4287
SfcI C'TryA_G
Cuts at: 0 1418 2236 2318 2933 3890 4033 4449
Size: 1418 818 82 615 957 143 416
Fragments arranged by size:
1418 957 818 615 416 143 82
SfoI GGC'GCC
Cuts at: 0 5 3910 4449
Size: 5 3905 539
Fragments arranged by size:
3905 539 5
SmaI CCC'GGG
Cuts at: 0 103 3247 3914 4449
Size: 103 3144 667 535
Fragments arranged by size:
3144 667 535 103
SmlI C'TyrA_G
Cuts at: 0 388 1711 1848 1920 4449
Size: 388 1323 137 72 2529
Fragments arranged by size:
2529 1323 388 137 72
SpeI A'CTAG_T
Cuts at: 0 2685 4449
Size: 2685 1764
SspI AAT'ATT
Cuts at: 0 1636 4449
Size: 1636 2813
StuI AGG'CCT
Cuts at: 0 91 4449
Size: 91 4358
StyI C'CwwG_G
Cuts at: 0 811 1381 1832 2675 3327 3950 4148 4449
Size: 811 570 451 843 652 623 198 301
Fragments arranged by size:
843 811 652 623 570 451 301 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2964 4355 4449
Size: 2964 1391 94
Fragments arranged by size:
2964 1391 94
TatI w'GTAC_w
Cuts at: 0 160 4449
Size: 160 4289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1176 1208 4449
Size: 1176 32 3241
Fragments arranged by size:
3241 1176 32
XbaI T'CTAG_A
Cuts at: 0 1095 1584 4449
Size: 1095 489 2865
Fragments arranged by size:
2865 1095 489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 1753 2836 3671 4449
Size: 342 1411 1083 835 778
Fragments arranged by size:
1411 1083 835 778 342
XmaI C'CCGG_G
Cuts at: 0 101 3245 3912 4449
Size: 101 3144 667 537
Fragments arranged by size:
3144 667 537 101
XmnI GAAnn'nnTTC
Cuts at: 0 272 2656 4449
Size: 272 2384 1793
Fragments arranged by size:
2384 1793 272
ZraI GAC'GTC
Cuts at: 0 2222 4449
Size: 2222 2227
Enzymes that do cut:
AarI AatII Acc65I AcuI AlfI AloI AlwNI ApoI
AseI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI PfoI PmlI PpuMI PstI
PvuII SacI SapI ScaI SfcI SfoI SmaI SmlI
SpeI SspI StuI StyI TaqII TatI TstI XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AleI
ApaI ApaLI AscI AsiSI BaeI BmrI BmtI BsaBI
BsiEI BsiWI BspEI BsrGI BssHII BstBI BstEII BstZ17I
BtgZI ClaI DraIII EagI EciI EcoRV FspAI MfeI
MluI NheI NotI NruI NspI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SnaBI SphI SrfI SwaI Tth111I XhoI