(Linear) (Six-base) MAPSORT of: KIEE0603.seq  Check: 3588  from: 1  to: 468



KIEE0603.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     127     162     176     468 
   Size:         37      90      35      14     292

  Fragments arranged by size: 

                 292      90      37      35      14
 
AflIII A'CryG_T

Cuts at:      0     310     468 
   Size:        310     158
 
BlpI GC'TnA_GC

Cuts at:      0      49     468 
   Size:         49     419
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     351     468 
   Size:        351     117
 
BsaAI yAC'GTr

Cuts at:      0      42     468 
   Size:         42     426
 
BsaBI GATnn'nnATC

Cuts at:      0     410     468 
   Size:        410      58
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     452     468 
   Size:        452      16
 
BseYI C'CCAG_C

Cuts at:      0     430     468 
   Size:        430      38
 
BsiEI CG_ry'CG

Cuts at:      0     378     468 
   Size:        378      90
 
BtgI C'CryG_G

Cuts at:      0     378     468 
   Size:        378      90
 
EaeI y'GGCC_r

Cuts at:      0     375     468 
   Size:        375      93
 
EagI C'GGCC_G

Cuts at:      0     375     468 
   Size:        375      93
 
EarI CTCTTCn'nnn_

Cuts at:      0     181     468 
   Size:        181     287
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     127     162     176     468 
   Size:         37      90      35      14     292

  Fragments arranged by size: 

                 292      90      37      35      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      16     468 
   Size:         16     452
 
FspI TGC'GCA

Cuts at:      0      28     468 
   Size:         28     440
 
HaeII r_GCGC'y

Cuts at:      0      25     468 
   Size:         25     443
 
MluI A'CGCG_T

Cuts at:      0     310     468 
   Size:        310     158
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     468 
   Size:        111     357
 
NspI r_CATG'y

Cuts at:      0     242     468 
   Size:        242     226
 
SspI AAT'ATT

Cuts at:      0     262     468 
   Size:        262     206
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     338     468 
   Size:        338     130
 
XmnI GAAnn'nnTTC

Cuts at:      0     138     468 
   Size:        138     330
 

 Enzymes that do cut:

     AcuI   AflIII     BlpI     BmrI    BsaAI    BsaBI    BseRI    BseYI
    BsiEI     BtgI     EaeI     EagI     EarI  Eco57MI    EcoNI     FspI
    HaeII     MluI     MmeI     NspI     SspI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BsaXI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI
     FseI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI