(Linear) (Six-base) MAPSORT of: KIEE0604.seq  Check: 459  from: 1  to: 1077



KIEE0604.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     641    1077 
   Size:        163     478     436

  Fragments arranged by size: 

                 478     436     163
 
AlwNI CAG_nnn'CTG

Cuts at:      0     639    1077 
   Size:        639     438
 
ApoI r'AATT_y

Cuts at:      0     105     184     487     643     776    1077 
   Size:        105      79     303     156     133     301

  Fragments arranged by size: 

                 303     301     156     133     105      79
 
BanI G'GyrC_C

Cuts at:      0    1041    1077 
   Size:       1041      36
 
BanII G_rGCy'C

Cuts at:      0     312    1077 
   Size:        312     765
 
BclI T'GATC_A

Cuts at:      0     720    1077 
   Size:        720     357
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     306    1077 
   Size:        306     771
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     800     947    1077 
   Size:        230     570     147     130

  Fragments arranged by size: 

                 570     230     147     130
 
BsaBI GATnn'nnATC

Cuts at:      0     719    1077 
   Size:        719     358
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       9      39    1077 
   Size:          9      30    1038

  Fragments arranged by size: 

                1038      30       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      12     713    1077 
   Size:         12     701     364

  Fragments arranged by size: 

                 701     364      12
 
BseYI C'CCAG_C

Cuts at:      0      10     238    1077 
   Size:         10     228     839

  Fragments arranged by size: 

                 839     228      10
 
BsiHKAI G_wGCw'C

Cuts at:      0     429     816     886    1077 
   Size:        429     387      70     191

  Fragments arranged by size: 

                 429     387     191      70
 
Bsp1286I G_dGCh'C

Cuts at:      0     312     429     816     886    1077 
   Size:        312     117     387      70     191

  Fragments arranged by size: 

                 387     312     191     117      70
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1038    1077 
   Size:       1038      39
 
Bsu36I CC'TnA_GG

Cuts at:      0      41     680     870    1077 
   Size:         41     639     190     207

  Fragments arranged by size: 

                 639     207     190      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     797    1077 
   Size:        797     280
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     230     641     800     947    1077 
   Size:        163      67     411     159     147     130

  Fragments arranged by size: 

                 411     163     159     147     130      67
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      40    1077 
   Size:         40    1037
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     151     183     649     681     931     963    1077 
   Size:        151      32     466      32     250      32     114

  Fragments arranged by size: 

                 466     250     151     114      32      32      32
 
HincII GTy'rAC

Cuts at:      0     760    1077 
   Size:        760     317
 
MfeI C'AATT_G

Cuts at:      0     323    1077 
   Size:        323     754
 
MspA1I CmG'CkG

Cuts at:      0     639    1077 
   Size:        639     438
 
PvuII CAG'CTG

Cuts at:      0     639    1077 
   Size:        639     438
 
SfcI C'TryA_G

Cuts at:      0     504    1077 
   Size:        504     573
 
TatI w'GTAC_w

Cuts at:      0     922    1077 
   Size:        922     155
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       9      41    1077 
   Size:          9      32    1036

  Fragments arranged by size: 

                1036      32       9
 
XmnI GAAnn'nnTTC

Cuts at:      0     788    1077 
   Size:        788     289
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BanI    BanII     BclI     BmrI     BpmI
    BsaBI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BstXI   Bsu36I
     EarI  Eco57MI    EcoNI    Hin4I   HincII     MfeI   MspA1I    PvuII
     SfcI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI