Restriction map for
pF1KE0606
(Linear) (Six-base) MAPSORT of: KIEE0606.seq Check: 2104 from: 1 to: 1359
KIEE0606.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 729 1059 1359
Size: 729 330 300
Fragments arranged by size:
729 330 300
AccI GT'mk_AC
Cuts at: 0 689 1359
Size: 689 670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1359
Size: 405 954
AfeI AGC'GCT
Cuts at: 0 397 1359
Size: 397 962
AflII C'TTAA_G
Cuts at: 0 1227 1359
Size: 1227 132
AleI CACnn'nnGTG
Cuts at: 0 166 1359
Size: 166 1193
AlwNI CAG_nnn'CTG
Cuts at: 0 29 171 647 1074 1359
Size: 29 142 476 427 285
Fragments arranged by size:
476 427 285 142 29
AvaI C'yCGr_G
Cuts at: 0 18 61 604 1282 1359
Size: 18 43 543 678 77
Fragments arranged by size:
678 543 77 43 18
BanI G'GyrC_C
Cuts at: 0 676 1190 1359
Size: 676 514 169
Fragments arranged by size:
676 514 169
BanII G_rGCy'C
Cuts at: 0 766 1256 1298 1359
Size: 766 490 42 61
Fragments arranged by size:
766 490 61 42
BbeI G_GCGC'C
Cuts at: 0 680 1194 1359
Size: 680 514 165
Fragments arranged by size:
680 514 165
BbvCI CC'TCA_GC
Cuts at: 0 796 1003 1359
Size: 796 207 356
Fragments arranged by size:
796 356 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1033 1067 1359
Size: 1033 34 292
Fragments arranged by size:
1033 292 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 178 1359
Size: 178 1181
BclI T'GATC_A
Cuts at: 0 45 113 1359
Size: 45 68 1246
Fragments arranged by size:
1246 68 45
BglI GCCn_nnn'nGGC
Cuts at: 0 20 496 761 1359
Size: 20 476 265 598
Fragments arranged by size:
598 476 265 20
Bme1580I G_kGCm'C
Cuts at: 0 551 781 1359
Size: 551 230 578
Fragments arranged by size:
578 551 230
BmgBI CAC'GTC
Cuts at: 0 302 1359
Size: 302 1057
BmrI ACTGGGnnnn_n'
Cuts at: 0 1251 1263 1359
Size: 1251 12 96
Fragments arranged by size:
1251 96 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1317 1359
Size: 1317 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 624 1359
Size: 564 60 735
Fragments arranged by size:
735 564 60
Bpu10I CC'TnA_GC
Cuts at: 0 796 1003 1359
Size: 796 207 356
Fragments arranged by size:
796 356 207
BsaI GGTCTCn'nnnn_
Cuts at: 0 568 601 1359
Size: 568 33 758
Fragments arranged by size:
758 568 33
BsaAI yAC'GTr
Cuts at: 0 375 1359
Size: 375 984
BsaBI GATnn'nnATC
Cuts at: 0 88 1359
Size: 88 1271
BsaHI Gr'CG_yC
Cuts at: 0 677 1191 1359
Size: 677 514 168
Fragments arranged by size:
677 514 168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 771 801 1359
Size: 29 742 30 558
Fragments arranged by size:
742 558 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 964 1359
Size: 964 395
BseYI C'CCAG_C
Cuts at: 0 10 550 1359
Size: 10 540 809
Fragments arranged by size:
809 540 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 355 1087 1183 1359
Size: 313 42 732 96 176
Fragments arranged by size:
732 313 176 96 42
BsiHKAI G_wGCw'C
Cuts at: 0 746 1359
Size: 746 613
BsmBI CGTCTCn'nnnn_
Cuts at: 0 982 1359
Size: 982 377
Bsp1286I G_dGCh'C
Cuts at: 0 551 746 766 781 1256 1298 1359
Size: 551 195 20 15 475 42 61
Fragments arranged by size:
551 475 195 61 42 20 15
BspHI T'CATG_A
Cuts at: 0 746 1359
Size: 746 613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 729 1059 1359
Size: 729 330 300
Fragments arranged by size:
729 330 300
BsrBI CCG'CTC
Cuts at: 0 578 1023 1359
Size: 578 445 336
Fragments arranged by size:
578 445 336
BsrGI T'GTAC_A
Cuts at: 0 1028 1359
Size: 1028 331
BssSI C'ACGA_G
Cuts at: 0 595 694 1108 1359
Size: 595 99 414 251
Fragments arranged by size:
595 414 251 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 29 1359
Size: 29 1330
BstEII G'GTnAC_C
Cuts at: 0 159 480 1359
Size: 159 321 879
Fragments arranged by size:
879 321 159
Bsu36I CC'TnA_GG
Cuts at: 0 853 1359
Size: 853 506
BtgI C'CryG_G
Cuts at: 0 263 518 1359
Size: 263 255 841
Fragments arranged by size:
841 263 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 387 1359
Size: 387 972
BtsI GCAGTG_nn'
Cuts at: 0 30 499 1359
Size: 30 469 860
Fragments arranged by size:
860 469 30
DraIII CAC_nnn'GTG
Cuts at: 0 216 1359
Size: 216 1143
EaeI y'GGCC_r
Cuts at: 0 74 770 875 920 1359
Size: 74 696 105 45 439
Fragments arranged by size:
696 439 105 74 45
EarI CTCTTCn'nnn_
Cuts at: 0 346 809 1183 1359
Size: 346 463 374 176
Fragments arranged by size:
463 374 346 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 477 1359
Size: 477 882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 564 624 1359
Size: 405 159 60 735
Fragments arranged by size:
735 405 159 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 343 1203 1359
Size: 343 860 156
Fragments arranged by size:
860 343 156
EcoO109I rG'GnC_Cy
Cuts at: 0 442 947 1359
Size: 442 505 412
Fragments arranged by size:
505 442 412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1242 1359
Size: 1242 117
FspI TGC'GCA
Cuts at: 0 1133 1359
Size: 1133 226
FspAI rTGC'GCAy
Cuts at: 0 1133 1359
Size: 1133 226
HaeII r_GCGC'y
Cuts at: 0 149 399 680 1119 1154 1170 1194 1359
Size: 149 250 281 439 35 16 24 165
Fragments arranged by size:
439 281 250 165 149 35 24 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1243 1275 1359
Size: 1243 32 84
Fragments arranged by size:
1243 84 32
KasI G'GCGC_C
Cuts at: 0 676 1190 1359
Size: 676 514 169
Fragments arranged by size:
676 514 169
MscI TGG'CCA
Cuts at: 0 76 1359
Size: 76 1283
MslI CAynn'nnrTG
Cuts at: 0 53 121 166 241 256 262 1359
Size: 53 68 45 75 15 6 1097
Fragments arranged by size:
1097 75 68 53 45 15 6
MspA1I CmG'CkG
Cuts at: 0 647 795 1359
Size: 647 148 564
Fragments arranged by size:
647 564 148
NarI GG'CG_CC
Cuts at: 0 677 1191 1359
Size: 677 514 168
Fragments arranged by size:
677 514 168
NcoI C'CATG_G
Cuts at: 0 263 518 1359
Size: 263 255 841
Fragments arranged by size:
841 263 255
NspI r_CATG'y
Cuts at: 0 261 1132 1359
Size: 261 871 227
Fragments arranged by size:
871 261 227
PflMI CCAn_nnn'nTGG
Cuts at: 0 647 1359
Size: 647 712
PmlI CAC'GTG
Cuts at: 0 375 1359
Size: 375 984
PpuMI rG'GwC_Cy
Cuts at: 0 442 947 1359
Size: 442 505 412
Fragments arranged by size:
505 442 412
PstI C_TGCA'G
Cuts at: 0 176 355 1097 1332 1359
Size: 176 179 742 235 27
Fragments arranged by size:
742 235 179 176 27
PvuII CAG'CTG
Cuts at: 0 795 1359
Size: 795 564
SapI GCTCTTCn'nnn_
Cuts at: 0 1183 1359
Size: 1183 176
SfcI C'TryA_G
Cuts at: 0 172 273 351 1093 1328 1359
Size: 172 101 78 742 235 31
Fragments arranged by size:
742 235 172 101 78 31
SfoI GGC'GCC
Cuts at: 0 678 1192 1359
Size: 678 514 167
Fragments arranged by size:
678 514 167
SmaI CCC'GGG
Cuts at: 0 20 1359
Size: 20 1339
SmlI C'TyrA_G
Cuts at: 0 1227 1282 1359
Size: 1227 55 77
Fragments arranged by size:
1227 77 55
SphI G_CATG'C
Cuts at: 0 261 1359
Size: 261 1098
StuI AGG'CCT
Cuts at: 0 349 1359
Size: 349 1010
StyI C'CwwG_G
Cuts at: 0 263 518 1359
Size: 263 255 841
Fragments arranged by size:
841 263 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 178 999 1359
Size: 24 154 821 360
Fragments arranged by size:
821 360 154 24
TatI w'GTAC_w
Cuts at: 0 1028 1359
Size: 1028 331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 771 803 1359
Size: 31 740 32 556
Fragments arranged by size:
740 556 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 300 945 1359
Size: 300 645 414
Fragments arranged by size:
645 414 300
XhoI C'TCGA_G
Cuts at: 0 1282 1359
Size: 1282 77
XmaI C'CCGG_G
Cuts at: 0 18 1359
Size: 18 1341
XmnI GAAnn'nnTTC
Cuts at: 0 1222 1359
Size: 1222 137
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AleI AlwNI AvaI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrGI BssSI BstAPI BstEII
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I
KasI MscI MslI MspA1I NarI NcoI NspI PflMI
PmlI PpuMI PstI PvuII SapI SfcI SfoI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AhdI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BfrBI BglII BlpI BmtI BpuEI BsaWI
BsiEI BsiWI BsmI BspEI BsrDI BsrFI BssHII BstBI
BstXI BstYI BstZ17I ClaI CspCI DraI DrdI EagI
EcoICRI EcoRI EcoRV FseI HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XcmI
ZraI