Restriction map for
pF1KE0608
(Linear) (Six-base) MAPSORT of: KIEE0608.seq Check: 115 from: 1 to: 1077
KIEE0608.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 1077
Size: 804 273
AflIII A'CryG_T
Cuts at: 0 787 1077
Size: 787 290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 642 1077
Size: 642 435
ApoI r'AATT_y
Cuts at: 0 272 1027 1077
Size: 272 755 50
Fragments arranged by size:
755 272 50
AvaI C'yCGr_G
Cuts at: 0 158 1077
Size: 158 919
BamHI G'GATC_C
Cuts at: 0 931 1077
Size: 931 146
BanI G'GyrC_C
Cuts at: 0 814 1077
Size: 814 263
BanII G_rGCy'C
Cuts at: 0 754 1077
Size: 754 323
BclI T'GATC_A
Cuts at: 0 449 1077
Size: 449 628
BglII A'GATC_T
Cuts at: 0 577 1077
Size: 577 500
BlpI GC'TnA_GC
Cuts at: 0 748 1077
Size: 748 329
BmgBI CAC'GTC
Cuts at: 0 788 1077
Size: 788 289
BmrI ACTGGGnnnn_n'
Cuts at: 0 626 1077
Size: 626 451
BmtI G_CTAG'C
Cuts at: 0 136 1077
Size: 136 941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 1077
Size: 789 288
BsaI GGTCTCn'nnnn_
Cuts at: 0 221 1077
Size: 221 856
BsaWI w'CCGG_w
Cuts at: 0 460 707 905 1077
Size: 460 247 198 172
Fragments arranged by size:
460 247 198 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 900 930 1077
Size: 29 871 30 147
Fragments arranged by size:
871 147 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 300 897 1077
Size: 300 597 180
Fragments arranged by size:
597 300 180
BseYI C'CCAG_C
Cuts at: 0 58 354 693 1077
Size: 58 296 339 384
Fragments arranged by size:
384 339 296 58
BsiHKAI G_wGCw'C
Cuts at: 0 415 754 1077
Size: 415 339 323
Fragments arranged by size:
415 339 323
BsmI GAATG_Cn'
Cuts at: 0 306 1077
Size: 306 771
Bsp1286I G_dGCh'C
Cuts at: 0 415 754 1077
Size: 415 339 323
Fragments arranged by size:
415 339 323
BspEI T'CCGG_A
Cuts at: 0 460 1077
Size: 460 617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 476 525 1077
Size: 476 49 552
Fragments arranged by size:
552 476 49
BsrDI GCAATG_nn'
Cuts at: 0 39 1077
Size: 39 1038
BsrFI r'CCGG_y
Cuts at: 0 91 1077
Size: 91 986
BstXI CCAn_nnnn'nTGG
Cuts at: 0 762 1077
Size: 762 315
BstYI r'GATC_y
Cuts at: 0 577 931 1077
Size: 577 354 146
Fragments arranged by size:
577 354 146
ClaI AT'CG_AT
Cuts at: 0 714 1077
Size: 714 363
DraI TTT'AAA
Cuts at: 0 542 1077
Size: 542 535
DrdI GACnn_nn'nnGTC
Cuts at: 0 792 1077
Size: 792 285
EarI CTCTTCn'nnn_
Cuts at: 0 985 1052 1077
Size: 985 67 25
Fragments arranged by size:
985 67 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 61 1077
Size: 61 1016
EcoICRI GAG'CTC
Cuts at: 0 752 1077
Size: 752 325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 804 1077
Size: 789 15 273
Fragments arranged by size:
789 273 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 686 1077
Size: 686 391
EcoO109I rG'GnC_Cy
Cuts at: 0 287 977 1077
Size: 287 690 100
Fragments arranged by size:
690 287 100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1038 1077
Size: 1038 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 468 500 562 594 646 678 1077
Size: 468 32 62 32 52 32 399
Fragments arranged by size:
468 399 62 52 32 32 32
HincII GTy'rAC
Cuts at: 0 444 1077
Size: 444 633
HindIII A'AGCT_T
Cuts at: 0 226 1077
Size: 226 851
MslI CAynn'nnrTG
Cuts at: 0 614 760 1077
Size: 614 146 317
Fragments arranged by size:
614 317 146
MspA1I CmG'CkG
Cuts at: 0 734 929 1077
Size: 734 195 148
Fragments arranged by size:
734 195 148
NheI G'CTAG_C
Cuts at: 0 132 1077
Size: 132 945
NspI r_CATG'y
Cuts at: 0 972 1077
Size: 972 105
PpuMI rG'GwC_Cy
Cuts at: 0 977 1077
Size: 977 100
PstI C_TGCA'G
Cuts at: 0 536 587 1077
Size: 536 51 490
Fragments arranged by size:
536 490 51
PvuII CAG'CTG
Cuts at: 0 734 1077
Size: 734 343
SacI G_AGCT'C
Cuts at: 0 754 1077
Size: 754 323
SanDI GG'GwC_CC
Cuts at: 0 977 1077
Size: 977 100
SapI GCTCTTCn'nnn_
Cuts at: 0 985 1077
Size: 985 92
SfcI C'TryA_G
Cuts at: 0 532 583 1077
Size: 532 51 494
Fragments arranged by size:
532 494 51
StyI C'CwwG_G
Cuts at: 0 860 1077
Size: 860 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 555 1077
Size: 555 522
TatI w'GTAC_w
Cuts at: 0 572 835 1077
Size: 572 263 242
Fragments arranged by size:
572 263 242
Enzymes that do cut:
AcuI AflIII AlfI ApoI AvaI BamHI BanI BanII
BclI BglII BlpI BmgBI BmrI BmtI BpmI BsaI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrDI BsrFI BstXI BstYI ClaI DraI DrdI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
HincII HindIII MslI MspA1I NheI NspI PpuMI PstI
PvuII SacI SanDI SapI SfcI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI Bme1580I BplI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsgI BsiEI BsiWI BsmBI BspHI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI