(Linear) (Six-base) MAPSORT of: KIEE0610.seq  Check: 9506  from: 1  to: 1089



KIEE0610.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     875    1089 
   Size:        875     214
 
AclI AA'CG_TT

Cuts at:      0    1025    1089 
   Size:       1025      64
 
AlwNI CAG_nnn'CTG

Cuts at:      0     511    1089 
   Size:        511     578
 
ApoI r'AATT_y

Cuts at:      0     225    1089 
   Size:        225     864
 
AseI AT'TA_AT

Cuts at:      0     982    1089 
   Size:        982     107
 
BclI T'GATC_A

Cuts at:      0     219    1089 
   Size:        219     870
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     384    1089 
   Size:        384     705
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     459    1089 
   Size:        459     630
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     459     504     557    1089 
   Size:        459      45      53     532

  Fragments arranged by size: 

                 532     459      53      45
 
Bpu10I CC'TnA_GC

Cuts at:      0     882    1089 
   Size:        882     207
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     602    1089 
   Size:        602     487
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     384     544     741     838    1089 
   Size:        384     160     197      97     251

  Fragments arranged by size: 

                 384     251     197     160      97
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     364     394    1089 
   Size:        364      30     695

  Fragments arranged by size: 

                 695     364      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      99     448     641    1028    1089 
   Size:         99     349     193     387      61

  Fragments arranged by size: 

                 387     349     193      99      61
 
BseYI C'CCAG_C

Cuts at:      0     154    1089 
   Size:        154     935
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295    1089 
   Size:        295     794
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     875    1089 
   Size:        875     214
 
BsrDI GCAATG_nn'

Cuts at:      0     683    1089 
   Size:        683     406
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     273    1089 
   Size:        273     816
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     739    1089 
   Size:        739     350
 
DraI TTT'AAA

Cuts at:      0     288    1089 
   Size:        288     801
 
EaeI y'GGCC_r

Cuts at:      0     348    1089 
   Size:        348     741
 
EarI CTCTTCn'nnn_

Cuts at:      0      13     472     626    1089 
   Size:         13     459     154     463

  Fragments arranged by size: 

                 463     459     154      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     459     504     557    1089 
   Size:        459      45      53     532

  Fragments arranged by size: 

                 532     459      53      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     148    1089 
   Size:        148     941
 
EcoO109I rG'GnC_Cy

Cuts at:      0     858    1089 
   Size:        858     231
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     156     188     295     327     364     396     721     753 
   Size:        156      32     107      32      37      32     325      32

Cuts at:    753    1089 
   Size:        336

  Fragments arranged by size: 

                 336     325     156     107      37      32      32      32
                  32
 
MfeI C'AATT_G

Cuts at:      0     970    1089 
   Size:        970     119
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     653    1089 
   Size:        653     436
 
MscI TGG'CCA

Cuts at:      0     350    1089 
   Size:        350     739
 
MslI CAynn'nnrTG

Cuts at:      0     299    1089 
   Size:        299     790
 
MspA1I CmG'CkG

Cuts at:      0     102    1089 
   Size:        102     987
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     535    1089 
   Size:        535     554
 
PfoI T'CCnGG_A

Cuts at:      0     573    1089 
   Size:        573     516
 
PpuMI rG'GwC_Cy

Cuts at:      0     858    1089 
   Size:        858     231
 
PsiI TTA'TAA

Cuts at:      0     185    1089 
   Size:        185     904
 
PvuII CAG'CTG

Cuts at:      0     102    1089 
   Size:        102     987
 
SexAI A'CCwGG_T

Cuts at:      0     731    1089 
   Size:        731     358
 
SmlI C'TyrA_G

Cuts at:      0     617    1089 
   Size:        617     472
 
StyI C'CwwG_G

Cuts at:      0     486    1089 
   Size:        486     603
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     190     222    1089 
   Size:        190      32     867

  Fragments arranged by size: 

                 867     190      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     376     733    1089 
   Size:        376     357     356

  Fragments arranged by size: 

                 376     357     356
 
XmnI GAAnn'nnTTC

Cuts at:      0    1027    1089 
   Size:       1027      62
 

 Enzymes that do cut:

     AarI     AclI    AlwNI     ApoI     AseI     BclI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI     BsgI
    BspMI    BsrDI   BstAPI    BstXI     DraI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    Hin4I     MfeI     MmeI     MscI     MslI   MspA1I
    PflMI     PfoI    PpuMI     PsiI    PvuII    SexAI     SmlI     StyI
     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     XbaI     XcmI     XhoI     XmaI
     ZraI