Restriction map for
pF1KE0611
(Linear) (Six-base) MAPSORT of: KIEE0611.seq Check: 7124 from: 1 to: 516
KIEE0611.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 0 236 516
Size: 236 280
Acc65I G'GTAC_C
Cuts at: 0 275 516
Size: 275 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 516
Size: 119 397
AgeI A'CCGG_T
Cuts at: 0 377 516
Size: 377 139
AlwNI CAG_nnn'CTG
Cuts at: 0 63 516
Size: 63 453
AvaI C'yCGr_G
Cuts at: 0 137 465 516
Size: 137 328 51
Fragments arranged by size:
328 137 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 267 300 516
Size: 267 33 216
Fragments arranged by size:
267 216 33
BanI G'GyrC_C
Cuts at: 0 275 516
Size: 275 241
BanII G_rGCy'C
Cuts at: 0 466 516
Size: 466 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 516
Size: 66 450
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 233 267 516
Size: 233 34 249
Fragments arranged by size:
249 233 34
BglI GCCn_nnn'nGGC
Cuts at: 0 193 516
Size: 193 323
BglII A'GATC_T
Cuts at: 0 92 133 516
Size: 92 41 383
Fragments arranged by size:
383 92 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 396 516
Size: 85 311 120
Fragments arranged by size:
311 120 85
Bpu10I CC'TnA_GC
Cuts at: 0 179 516
Size: 179 337
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 516
Size: 118 398
BsaWI w'CCGG_w
Cuts at: 0 377 458 516
Size: 377 81 58
Fragments arranged by size:
377 81 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 426 456 516
Size: 394 30 2 30 60
Fragments arranged by size:
394 60 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 362 365 516
Size: 362 3 151
Fragments arranged by size:
362 151 3
BsiHKAI G_wGCw'C
Cuts at: 0 466 516
Size: 466 50
Bsp1286I G_dGCh'C
Cuts at: 0 466 516
Size: 466 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 60 516
Size: 60 456
BsrFI r'CCGG_y
Cuts at: 0 377 516
Size: 377 139
BstYI r'GATC_y
Cuts at: 0 92 133 227 516
Size: 92 41 94 289
Fragments arranged by size:
289 94 92 41
BtgI C'CryG_G
Cuts at: 0 270 279 312 333 516
Size: 270 9 33 21 183
Fragments arranged by size:
270 183 33 21 9
EaeI y'GGCC_r
Cuts at: 0 194 516
Size: 194 322
EcoICRI GAG'CTC
Cuts at: 0 464 516
Size: 464 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 119 396 516
Size: 85 34 277 120
Fragments arranged by size:
277 120 85 34
EcoO109I rG'GnC_Cy
Cuts at: 0 473 516
Size: 473 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 37 516
Size: 37 479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 212 244 250 282 448 480 516
Size: 212 32 6 32 166 32 36
Fragments arranged by size:
212 166 36 32 32 32 6
KpnI G_GTAC'C
Cuts at: 0 279 516
Size: 279 237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 516
Size: 9 507
MscI TGG'CCA
Cuts at: 0 196 516
Size: 196 320
PfoI T'CCnGG_A
Cuts at: 0 388 516
Size: 388 128
PpuMI rG'GwC_Cy
Cuts at: 0 473 516
Size: 473 43
SacI G_AGCT'C
Cuts at: 0 466 516
Size: 466 50
SfcI C'TryA_G
Cuts at: 0 212 423 450 477 516
Size: 212 211 27 27 39
Fragments arranged by size:
212 211 39 27 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 374 516
Size: 374 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 426 458 516
Size: 426 32 58
Fragments arranged by size:
426 58 32
XbaI T'CTAG_A
Cuts at: 0 343 516
Size: 343 173
XmnI GAAnn'nnTTC
Cuts at: 0 379 516
Size: 379 137
Enzymes that do cut:
AccI Acc65I AcuI AgeI AlwNI AvaI BaeI BanI
BanII BbsI BcgI BglI BglII BpmI Bpu10I BsaI
BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspMI BsrFI BstYI
BtgI EaeI EcoICRI Eco57MI EcoO109I FalI Hin4I KpnI
MmeI MscI PfoI PpuMI SacI SfcI TaqII TstI
XbaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BbvCI BciVI BclI BfrBI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI Tth111I XcmI XhoI
XmaI ZraI