(Linear) (Six-base) MAPSORT of: KIEE0614.seq  Check: 2841  from: 1  to: 1092



KIEE0614.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     790    1092 
   Size:        790     302
 
AflIII A'CryG_T

Cuts at:      0     842    1092 
   Size:        842     250
 
ApoI r'AATT_y

Cuts at:      0     880     901    1092 
   Size:        880      21     191

  Fragments arranged by size: 

                 880     191      21
 
BanI G'GyrC_C

Cuts at:      0     594    1092 
   Size:        594     498
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     615     757     778    1092 
   Size:        615     142      21     314

  Fragments arranged by size: 

                 615     314     142      21
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      70     104    1092 
   Size:         70      34     988

  Fragments arranged by size: 

                 988      70      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     744    1092 
   Size:        744     348
 
BmgBI CAC'GTC

Cuts at:      0     843     864    1092 
   Size:        843      21     228

  Fragments arranged by size: 

                 843     228      21
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      36     539    1092 
   Size:         36     503     553

  Fragments arranged by size: 

                 553     503      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     734    1092 
   Size:        734     358
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1082    1092 
   Size:       1082      10
 
BseYI C'CCAG_C

Cuts at:      0     292    1092 
   Size:        292     800
 
BsrBI CCG'CTC

Cuts at:      0    1028    1092 
   Size:       1028      64
 
BsrDI GCAATG_nn'

Cuts at:      0      86    1080    1092 
   Size:         86     994      12

  Fragments arranged by size: 

                 994      86      12
 
BsrFI r'CCGG_y

Cuts at:      0     482    1003    1092 
   Size:        482     521      89

  Fragments arranged by size: 

                 521     482      89
 
BstEII G'GTnAC_C

Cuts at:      0     645    1092 
   Size:        645     447
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     204     417     531    1092 
   Size:        204     213     114     561

  Fragments arranged by size: 

                 561     213     204     114
 
BstYI r'GATC_y

Cuts at:      0     971    1092 
   Size:        971     121
 
BtsI GCAGTG_nn'

Cuts at:      0     996    1092 
   Size:        996      96
 
DraIII CAC_nnn'GTG

Cuts at:      0    1086    1092 
   Size:       1086       6
 
EaeI y'GGCC_r

Cuts at:      0     194     407     521    1092 
   Size:        194     213     114     571

  Fragments arranged by size: 

                 571     213     194     114
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     756    1092 
   Size:        756     336
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     790    1082    1092 
   Size:        790     292      10

  Fragments arranged by size: 

                 790     292      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     370    1092 
   Size:        370     722
 
EcoO109I rG'GnC_Cy

Cuts at:      0     328     375     794    1092 
   Size:        328      47     419     298

  Fragments arranged by size: 

                 419     328     298      47
 
EcoRI G'AATT_C

Cuts at:      0     901    1092 
   Size:        901     191
 
HaeII r_GCGC'y

Cuts at:      0     959    1092 
   Size:        959     133
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     152     184    1092 
   Size:        152      32     908

  Fragments arranged by size: 

                 908     152      32
 
HincII GTy'rAC

Cuts at:      0     228    1092 
   Size:        228     864
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     611    1092 
   Size:        611     481
 
MscI TGG'CCA

Cuts at:      0     196     409     523    1092 
   Size:        196     213     114     569

  Fragments arranged by size: 

                 569     213     196     114
 
MslI CAynn'nnrTG

Cuts at:      0      52     472     529    1073    1092 
   Size:         52     420      57     544      19

  Fragments arranged by size: 

                 544     420      57      52      19
 
NspI r_CATG'y

Cuts at:      0     459    1092 
   Size:        459     633
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     131    1086    1092 
   Size:        131     955       6

  Fragments arranged by size: 

                 955     131       6
 
PfoI T'CCnGG_A

Cuts at:      0     816    1092 
   Size:        816     276
 
PpuMI rG'GwC_Cy

Cuts at:      0     328    1092 
   Size:        328     764
 
PshAI GACnn'nnGTC

Cuts at:      0     778    1092 
   Size:        778     314
 
PsiI TTA'TAA

Cuts at:      0     101    1092 
   Size:        101     991
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     650     682    1092 
   Size:        650      32     410

  Fragments arranged by size: 

                 650     410      32
 
PstI C_TGCA'G

Cuts at:      0     282    1092 
   Size:        282     810
 
SfcI C'TryA_G

Cuts at:      0     278     603    1092 
   Size:        278     325     489

  Fragments arranged by size: 

                 489     325     278
 
SphI G_CATG'C

Cuts at:      0     459    1092 
   Size:        459     633
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     523    1092 
   Size:        523     569
 
XbaI T'CTAG_A

Cuts at:      0     837    1092 
   Size:        837     255
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     639    1092 
   Size:        639     453
 
XmnI GAAnn'nnTTC

Cuts at:      0     883    1092 
   Size:        883     209
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     BanI     BbsI     BcgI    BciVI    BmgBI
     BmrI     BplI     BpmI    BseYI    BsrBI    BsrDI    BsrFI   BstEII
    BstXI    BstYI     BtsI   DraIII     EaeI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI    HaeII    Hin4I   HincII     MmeI     MscI     MslI
     NspI    PflMI     PfoI    PpuMI    PshAI     PsiI     PsrI     PstI
     SfcI     SphI    TaqII     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EagI     EarI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI   PspOMI    PspXI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XhoI     XmaI     ZraI