(Linear) (Six-base) MAPSORT of: KIEE0615.seq  Check: 6477  from: 1  to: 522



KIEE0615.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AatII G_ACGT'C

Cuts at:      0     519     522 
   Size:        519       3
 
AccI GT'mk_AC

Cuts at:      0     346     522 
   Size:        346     176
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     522 
   Size:        358     164
 
ApoI r'AATT_y

Cuts at:      0     274     522 
   Size:        274     248
 
AvaI C'yCGr_G

Cuts at:      0     137     522 
   Size:        137     385
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     266     495     522 
   Size:        266     229      27

  Fragments arranged by size: 

                 266     229      27
 
BglII A'GATC_T

Cuts at:      0      15     522 
   Size:         15     507
 
BmgBI CAC'GTC

Cuts at:      0       9     522 
   Size:          9     513
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     522 
   Size:        254     268
 
BsaHI Gr'CG_yC

Cuts at:      0     516     522 
   Size:        516       6
 
BsaWI w'CCGG_w

Cuts at:      0     509     522 
   Size:        509      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     489     522 
   Size:        489      33
 
BseYI C'CCAG_C

Cuts at:      0      28     522 
   Size:         28     494
 
BsmI GAATG_Cn'

Cuts at:      0      71     522 
   Size:         71     451
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     507     522 
   Size:        507      15
 
BssSI C'ACGA_G

Cuts at:      0      22     522 
   Size:         22     500
 
BstYI r'GATC_y

Cuts at:      0      15     522 
   Size:         15     507
 
BstZ17I GTA'TAC

Cuts at:      0     347     522 
   Size:        347     175
 
BtgI C'CryG_G

Cuts at:      0      47     522 
   Size:         47     475
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     197     522 
   Size:        197     325
 
EaeI y'GGCC_r

Cuts at:      0     405     483     522 
   Size:        405      78      39

  Fragments arranged by size: 

                 405      78      39
 
EarI CTCTTCn'nnn_

Cuts at:      0     122     522 
   Size:        122     400
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     522 
   Size:         19     503
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     358     522 
   Size:        254     104     164

  Fragments arranged by size: 

                 254     164     104
 
EcoO109I rG'GnC_Cy

Cuts at:      0     134     522 
   Size:        134     388
 
EcoRV GAT'ATC

Cuts at:      0     334     522 
   Size:        334     188
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     413     445     522 
   Size:        413      32      77

  Fragments arranged by size: 

                 413      77      32
 
HincII GTy'rAC

Cuts at:      0     438     522 
   Size:        438      84
 
MscI TGG'CCA

Cuts at:      0     407     522 
   Size:        407     115
 
NcoI C'CATG_G

Cuts at:      0      47     522 
   Size:         47     475
 
PpuMI rG'GwC_Cy

Cuts at:      0     134     522 
   Size:        134     388
 
SanDI GG'GwC_CC

Cuts at:      0     134     522 
   Size:        134     388
 
StyI C'CwwG_G

Cuts at:      0      47     522 
   Size:         47     475
 
ZraI GAC'GTC

Cuts at:      0     517     522 
   Size:        517       5
 

 Enzymes that do cut:

    AatII     AccI     AcuI     ApoI     AvaI     BbsI    BglII    BmgBI
     BpmI    BsaHI    BsaWI    BseRI    BseYI     BsmI    BsmBI    BssSI
    BstYI  BstZ17I     BtgI    BtgZI     EaeI     EarI     EciI  Eco57MI
 EcoO109I    EcoRV    Hin4I   HincII     MscI     NcoI    PpuMI    SanDI
     StyI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI