(Linear) (Six-base) MAPSORT of: KIEE0615.seq Check: 6477 from: 1 to: 522 KIEE0615.orf With 182 enzymes: SgfI * April 24, 2009 13:16 .. AatII G_ACGT'C Cuts at: 0 519 522 Size: 519 3 AccI GT'mk_AC Cuts at: 0 346 522 Size: 346 176 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 522 Size: 358 164 ApoI r'AATT_y Cuts at: 0 274 522 Size: 274 248 AvaI C'yCGr_G Cuts at: 0 137 522 Size: 137 385 BbsI GAAGACnn'nnnn_ Cuts at: 0 266 495 522 Size: 266 229 27 Fragments arranged by size: 266 229 27 BglII A'GATC_T Cuts at: 0 15 522 Size: 15 507 BmgBI CAC'GTC Cuts at: 0 9 522 Size: 9 513 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 522 Size: 254 268 BsaHI Gr'CG_yC Cuts at: 0 516 522 Size: 516 6 BsaWI w'CCGG_w Cuts at: 0 509 522 Size: 509 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 489 522 Size: 489 33 BseYI C'CCAG_C Cuts at: 0 28 522 Size: 28 494 BsmI GAATG_Cn' Cuts at: 0 71 522 Size: 71 451 BsmBI CGTCTCn'nnnn_ Cuts at: 0 507 522 Size: 507 15 BssSI C'ACGA_G Cuts at: 0 22 522 Size: 22 500 BstYI r'GATC_y Cuts at: 0 15 522 Size: 15 507 BstZ17I GTA'TAC Cuts at: 0 347 522 Size: 347 175 BtgI C'CryG_G Cuts at: 0 47 522 Size: 47 475 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 197 522 Size: 197 325 EaeI y'GGCC_r Cuts at: 0 405 483 522 Size: 405 78 39 Fragments arranged by size: 405 78 39 EarI CTCTTCn'nnn_ Cuts at: 0 122 522 Size: 122 400 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 522 Size: 19 503 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 358 522 Size: 254 104 164 Fragments arranged by size: 254 164 104 EcoO109I rG'GnC_Cy Cuts at: 0 134 522 Size: 134 388 EcoRV GAT'ATC Cuts at: 0 334 522 Size: 334 188 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 413 445 522 Size: 413 32 77 Fragments arranged by size: 413 77 32 HincII GTy'rAC Cuts at: 0 438 522 Size: 438 84 MscI TGG'CCA Cuts at: 0 407 522 Size: 407 115 NcoI C'CATG_G Cuts at: 0 47 522 Size: 47 475 PpuMI rG'GwC_Cy Cuts at: 0 134 522 Size: 134 388 SanDI GG'GwC_CC Cuts at: 0 134 522 Size: 134 388 StyI C'CwwG_G Cuts at: 0 47 522 Size: 47 475 ZraI GAC'GTC Cuts at: 0 517 522 Size: 517 5 Enzymes that do cut: AatII AccI AcuI ApoI AvaI BbsI BglII BmgBI BpmI BsaHI BsaWI BseRI BseYI BsmI BsmBI BssSI BstYI BstZ17I BtgI BtgZI EaeI EarI EciI Eco57MI EcoO109I EcoRV Hin4I HincII MscI NcoI PpuMI SanDI StyI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI