Restriction map for
pF1KE0618
(Linear) (Six-base) MAPSORT of: KIEE0618.seq Check: 1116 from: 1 to: 1098
KIEE0618.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 749 1098
Size: 749 349
AlwNI CAG_nnn'CTG
Cuts at: 0 267 675 882 1098
Size: 267 408 207 216
Fragments arranged by size:
408 267 216 207
ApaI G_GGCC'C
Cuts at: 0 363 1098
Size: 363 735
ApoI r'AATT_y
Cuts at: 0 146 1098
Size: 146 952
AvaI C'yCGr_G
Cuts at: 0 356 1098
Size: 356 742
BanII G_rGCy'C
Cuts at: 0 235 363 727 1098
Size: 235 128 364 371
Fragments arranged by size:
371 364 235 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 622 1098
Size: 622 476
BglII A'GATC_T
Cuts at: 0 225 1098
Size: 225 873
Bme1580I G_kGCm'C
Cuts at: 0 363 1098
Size: 363 735
BmgBI CAC'GTC
Cuts at: 0 642 1098
Size: 642 456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 739 964 1098
Size: 62 677 225 134
Fragments arranged by size:
677 225 134 62
Bpu10I CC'TnA_GC
Cuts at: 0 547 692 1098
Size: 547 145 406
Fragments arranged by size:
547 406 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 536 1098
Size: 536 562
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 715 1098
Size: 79 636 383
Fragments arranged by size:
636 383 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 895 1098
Size: 640 255 203
Fragments arranged by size:
640 255 203
BsmI GAATG_Cn'
Cuts at: 0 538 1098
Size: 538 560
Bsp1286I G_dGCh'C
Cuts at: 0 235 363 727 1098
Size: 235 128 364 371
Fragments arranged by size:
371 364 235 128
BstAPI GCAn_nnn'nTGC
Cuts at: 0 882 1098
Size: 882 216
BstYI r'GATC_y
Cuts at: 0 225 1098
Size: 225 873
Bsu36I CC'TnA_GG
Cuts at: 0 526 1098
Size: 526 572
BtgI C'CryG_G
Cuts at: 0 576 1098
Size: 576 522
EaeI y'GGCC_r
Cuts at: 0 860 1098
Size: 860 238
EarI CTCTTCn'nnn_
Cuts at: 0 286 1098
Size: 286 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 712 1098
Size: 712 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 739 964 1098
Size: 62 677 225 134
Fragments arranged by size:
677 225 134 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 160 691 792 1098
Size: 160 531 101 306
Fragments arranged by size:
531 306 160 101
EcoO109I rG'GnC_Cy
Cuts at: 0 320 332 360 506 1098
Size: 320 12 28 146 592
Fragments arranged by size:
592 320 146 28 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 268 1098
Size: 268 830
FspI TGC'GCA
Cuts at: 0 886 1098
Size: 886 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 217 249 735 767 1098
Size: 58 32 127 32 486 32 331
Fragments arranged by size:
486 331 127 58 32 32 32
HincII GTy'rAC
Cuts at: 0 444 1098
Size: 444 654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 353 473 1098
Size: 41 312 120 625
Fragments arranged by size:
625 312 120 41
MscI TGG'CCA
Cuts at: 0 862 1098
Size: 862 236
MslI CAynn'nnrTG
Cuts at: 0 733 871 1098
Size: 733 138 227
Fragments arranged by size:
733 227 138
MspA1I CmG'CkG
Cuts at: 0 366 1098
Size: 366 732
NspI r_CATG'y
Cuts at: 0 669 1098
Size: 669 429
PfoI T'CCnGG_A
Cuts at: 0 4 78 260 1098
Size: 4 74 182 838
Fragments arranged by size:
838 182 74 4
PpuMI rG'GwC_Cy
Cuts at: 0 332 506 1098
Size: 332 174 592
Fragments arranged by size:
592 332 174
PspOMI G'GGCC_C
Cuts at: 0 359 1098
Size: 359 739
PstI C_TGCA'G
Cuts at: 0 1085 1098
Size: 1085 13
SfcI C'TryA_G
Cuts at: 0 1081 1098
Size: 1081 17
StyI C'CwwG_G
Cuts at: 0 1070 1098
Size: 1070 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 792 1098
Size: 792 306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 242 274 1098
Size: 242 32 824
Fragments arranged by size:
824 242 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 268 574 1098
Size: 268 306 524
Fragments arranged by size:
524 306 268
Enzymes that do cut:
AhdI AlwNI ApaI ApoI AvaI BanII BbsI BglII
Bme1580I BmgBI BpmI Bpu10I BsaI BseRI BsgI BsmI
Bsp1286I BstAPI BstYI Bsu36I BtgI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI FspI Hin4I HincII MmeI
MscI MslI MspA1I NspI PfoI PpuMI PspOMI PstI
SfcI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FseI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI