Restriction map for
pF1KE0620
(Linear) (Six-base) MAPSORT of: KIEE0620.seq Check: 3916 from: 1 to: 1080
KIEE0620.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 195 1080
Size: 195 885
AlwNI CAG_nnn'CTG
Cuts at: 0 15 639 1080
Size: 15 624 441
Fragments arranged by size:
624 441 15
ApaI G_GGCC'C
Cuts at: 0 49 1080
Size: 49 1031
AvaI C'yCGr_G
Cuts at: 0 494 1080
Size: 494 586
BanI G'GyrC_C
Cuts at: 0 820 1080
Size: 820 260
BanII G_rGCy'C
Cuts at: 0 49 395 590 1080
Size: 49 346 195 490
Fragments arranged by size:
490 346 195 49
BbeI G_GCGC'C
Cuts at: 0 824 1080
Size: 824 256
BbsI GAAGACnn'nnnn_
Cuts at: 0 328 631 1080
Size: 328 303 449
Fragments arranged by size:
449 328 303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 939 973 1080
Size: 939 34 107
Fragments arranged by size:
939 107 34
BglI GCCn_nnn'nGGC
Cuts at: 0 53 58 72 541 791 1016 1080
Size: 53 5 14 469 250 225 64
Fragments arranged by size:
469 250 225 64 53 14 5
BlpI GC'TnA_GC
Cuts at: 0 584 1080
Size: 584 496
Bme1580I G_kGCm'C
Cuts at: 0 49 1080
Size: 49 1031
BmgBI CAC'GTC
Cuts at: 0 458 1080
Size: 458 622
BmrI ACTGGGnnnn_n'
Cuts at: 0 25 344 432 1080
Size: 25 319 88 648
Fragments arranged by size:
648 319 88 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 1053 1080
Size: 1053 27
BsaBI GATnn'nnATC
Cuts at: 0 766 1080
Size: 766 314
BsaHI Gr'CG_yC
Cuts at: 0 743 821 1080
Size: 743 78 259
Fragments arranged by size:
743 259 78
BsaWI w'CCGG_w
Cuts at: 0 855 1080
Size: 855 225
BseYI C'CCAG_C
Cuts at: 0 573 701 1080
Size: 573 128 379
Fragments arranged by size:
573 379 128
BsiEI CG_ry'CG
Cuts at: 0 53 447 764 1080
Size: 53 394 317 316
Fragments arranged by size:
394 317 316 53
Bsp1286I G_dGCh'C
Cuts at: 0 49 395 590 1080
Size: 49 346 195 490
Fragments arranged by size:
490 346 195 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 1035 1080
Size: 143 892 45
Fragments arranged by size:
892 143 45
BsrBI CCG'CTC
Cuts at: 0 782 1080
Size: 782 298
BsrFI r'CCGG_y
Cuts at: 0 52 823 1080
Size: 52 771 257
Fragments arranged by size:
771 257 52
BsrGI T'GTAC_A
Cuts at: 0 842 1080
Size: 842 238
BstAPI GCAn_nnn'nTGC
Cuts at: 0 639 1080
Size: 639 441
BtgI C'CryG_G
Cuts at: 0 23 995 1080
Size: 23 972 85
Fragments arranged by size:
972 85 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 987 1080
Size: 987 93
ClaI AT'CG_AT
Cuts at: 0 770 1080
Size: 770 310
EaeI y'GGCC_r
Cuts at: 0 50 444 992 998 1080
Size: 50 394 548 6 82
Fragments arranged by size:
548 394 82 50 6
EagI C'GGCC_G
Cuts at: 0 50 444 1080
Size: 50 394 636
Fragments arranged by size:
636 394 50
EarI CTCTTCn'nnn_
Cuts at: 0 178 436 533 1080
Size: 178 258 97 547
Fragments arranged by size:
547 258 178 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 654 1080
Size: 654 426
EcoO109I rG'GnC_Cy
Cuts at: 0 45 682 1080
Size: 45 637 398
Fragments arranged by size:
637 398 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 189 1080
Size: 189 891
FseI GG_CCGG'CC
Cuts at: 0 56 1080
Size: 56 1024
HaeII r_GCGC'y
Cuts at: 0 640 675 824 1080
Size: 640 35 149 256
Fragments arranged by size:
640 256 149 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 1080
Size: 181 32 867
Fragments arranged by size:
867 181 32
HincII GTy'rAC
Cuts at: 0 129 1080
Size: 129 951
KasI G'GCGC_C
Cuts at: 0 820 1080
Size: 820 260
MscI TGG'CCA
Cuts at: 0 1000 1080
Size: 1000 80
MslI CAynn'nnrTG
Cuts at: 0 331 469 838 1080
Size: 331 138 369 242
Fragments arranged by size:
369 331 242 138
MspA1I CmG'CkG
Cuts at: 0 389 573 583 885 1080
Size: 389 184 10 302 195
Fragments arranged by size:
389 302 195 184 10
NaeI GCC'GGC
Cuts at: 0 54 825 1080
Size: 54 771 255
Fragments arranged by size:
771 255 54
NarI GG'CG_CC
Cuts at: 0 821 1080
Size: 821 259
NcoI C'CATG_G
Cuts at: 0 23 995 1080
Size: 23 972 85
Fragments arranged by size:
972 85 23
NgoMIV G'CCGG_C
Cuts at: 0 52 823 1080
Size: 52 771 257
Fragments arranged by size:
771 257 52
NruI TCG'CGA
Cuts at: 0 607 1080
Size: 607 473
PflMI CCAn_nnn'nTGG
Cuts at: 0 82 1080
Size: 82 998
PfoI T'CCnGG_A
Cuts at: 0 365 1080
Size: 365 715
PspOMI G'GGCC_C
Cuts at: 0 45 1080
Size: 45 1035
PstI C_TGCA'G
Cuts at: 0 1046 1080
Size: 1046 34
PvuI CG_AT'CG
Cuts at: 0 764 1080
Size: 764 316
PvuII CAG'CTG
Cuts at: 0 583 885 1080
Size: 583 302 195
Fragments arranged by size:
583 302 195
SapI GCTCTTCn'nnn_
Cuts at: 0 436 1080
Size: 436 644
SexAI A'CCwGG_T
Cuts at: 0 476 1080
Size: 476 604
SfcI C'TryA_G
Cuts at: 0 1042 1080
Size: 1042 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 53 58 1080
Size: 53 5 1022
Fragments arranged by size:
1022 53 5
SfoI GGC'GCC
Cuts at: 0 822 1080
Size: 822 258
StyI C'CwwG_G
Cuts at: 0 23 81 237 995 1080
Size: 23 58 156 758 85
Fragments arranged by size:
758 156 85 58 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 1080
Size: 661 419
TatI w'GTAC_w
Cuts at: 0 842 1080
Size: 842 238
Tth111I GACn'n_nGTC
Cuts at: 0 478 1080
Size: 478 602
Enzymes that do cut:
AhdI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BcgI BglI BlpI Bme1580I BmgBI BmrI BsaI BsaBI
BsaHI BsaWI BseYI BsiEI Bsp1286I BspMI BsrBI BsrFI
BsrGI BstAPI BtgI BtgZI ClaI EaeI EagI EarI
EciI EcoO109I FalI FseI HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
PflMI PfoI PspOMI PstI PvuI PvuII SapI SexAI
SfcI SfiI SfoI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglII BmtI BplI BpmI Bpu10I BpuEI BsaAI
BsaXI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtsI CspCI DraI DraIII DrdI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NotI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI RsrII SacI SacII SalI
SanDI SbfI ScaI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XcmI XhoI XmaI XmnI ZraI