(Linear) (Six-base) MAPSORT of: KIEE0621.seq  Check: 103  from: 1  to: 570



KIEE0621.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     493     570 
   Size:        493      77
 
AvaI C'yCGr_G

Cuts at:      0     203     370     570 
   Size:        203     167     200

  Fragments arranged by size: 

                 203     200     167
 
BanII G_rGCy'C

Cuts at:      0      58     570 
   Size:         58     512
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     491     570 
   Size:        491      79
 
BclI T'GATC_A

Cuts at:      0     333     570 
   Size:        333     237
 
BfrBI ATG'CAT

Cuts at:      0      37     570 
   Size:         37     533
 
Bme1580I G_kGCm'C

Cuts at:      0     312     570 
   Size:        312     258
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     396     570 
   Size:        396     174
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     118     570 
   Size:         49      69     452

  Fragments arranged by size: 

                 452      69      49
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     302     366     570 
   Size:        109     193      64     204

  Fragments arranged by size: 

                 204     193     109      64
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     121     570 
   Size:        112       9     449

  Fragments arranged by size: 

                 449     112       9
 
BsmI GAATG_Cn'

Cuts at:      0      39     570 
   Size:         39     531
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     312     570 
   Size:         58     254     258

  Fragments arranged by size: 

                 258     254      58
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     142     294     570 
   Size:        142     152     276

  Fragments arranged by size: 

                 276     152     142
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     318     570 
   Size:        318     252
 
BtsI GCAGTG_nn'

Cuts at:      0     416     570 
   Size:        416     154
 
EarI CTCTTCn'nnn_

Cuts at:      0      26     175     570 
   Size:         26     149     395

  Fragments arranged by size: 

                 395     149      26
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     475     570 
   Size:        475      95
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     118     570 
   Size:         49      69     452

  Fragments arranged by size: 

                 452      69      49
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      49     570 
   Size:         49     521
 
HaeII r_GCGC'y

Cuts at:      0     493     570 
   Size:        493      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      22      54     318     350     570 
   Size:         22      32     264      32     220

  Fragments arranged by size: 

                 264     220      32      32      22
 
HincII GTy'rAC

Cuts at:      0     222     570 
   Size:        222     348
 
HpaI GTT'AAC

Cuts at:      0     222     570 
   Size:        222     348
 
NsiI A_TGCA'T

Cuts at:      0      39     570 
   Size:         39     531
 
NspI r_CATG'y

Cuts at:      0     316     570 
   Size:        316     254
 
PspXI vC'TCGA_Gb

Cuts at:      0     203     570 
   Size:        203     367
 
PstI C_TGCA'G

Cuts at:      0      65     570 
   Size:         65     505
 
SfcI C'TryA_G

Cuts at:      0      61     570 
   Size:         61     509
 
SmlI C'TyrA_G

Cuts at:      0     124     203     317     370     381     570 
   Size:        124      79     114      53      11     189

  Fragments arranged by size: 

                 189     124     114      79      53      11
 
XbaI T'CTAG_A

Cuts at:      0     544     570 
   Size:        544      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     265     294     570 
   Size:        265      29     276

  Fragments arranged by size: 

                 276     265      29
 
XhoI C'TCGA_G

Cuts at:      0     203     370     570 
   Size:        203     167     200

  Fragments arranged by size: 

                 203     200     167
 

 Enzymes that do cut:

    AlwNI     AvaI    BanII    BciVI     BclI    BfrBI Bme1580I     BplI
     BpmI    BpuEI    BseRI     BsmI Bsp1286I    BstXI    BtgZI     BtsI
     EarI     EciI  Eco57MI    EcoNI    HaeII    Hin4I   HincII     HpaI
     NsiI     NspI    PspXI     PstI     SfcI     SmlI     XbaI     XcmI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XmaI     XmnI     ZraI