(Linear) (Six-base) MAPSORT of: KIEE0629.seq  Check: 2838  from: 1  to: 594



KIEE0629.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AflIII A'CryG_T

Cuts at:      0     470     594 
   Size:        470     124
 
AgeI A'CCGG_T

Cuts at:      0     523     594 
   Size:        523      71
 
ApoI r'AATT_y

Cuts at:      0     307     583     594 
   Size:        307     276      11

  Fragments arranged by size: 

                 307     276      11
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     504     537     594 
   Size:        504      33      57

  Fragments arranged by size: 

                 504      57      33
 
BanI G'GyrC_C

Cuts at:      0      11     192     526     594 
   Size:         11     181     334      68

  Fragments arranged by size: 

                 334     181      68      11
 
BanII G_rGCy'C

Cuts at:      0      23     128     594 
   Size:         23     105     466

  Fragments arranged by size: 

                 466     105      23
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      99     133     594 
   Size:         99      34     461

  Fragments arranged by size: 

                 461      99      34
 
BmgBI CAC'GTC

Cuts at:      0     473     594 
   Size:        473     121
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     156     594 
   Size:        156     438
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     594 
   Size:         37     557
 
BsaWI w'CCGG_w

Cuts at:      0     523     594 
   Size:        523      71
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     594 
   Size:         94     500
 
BsiHKAI G_wGCw'C

Cuts at:      0     128     594 
   Size:        128     466
 
BsmI GAATG_Cn'

Cuts at:      0     228     594 
   Size:        228     366
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     128     594 
   Size:         23     105     466

  Fragments arranged by size: 

                 466     105      23
 
BsrBI CCG'CTC

Cuts at:      0     465     594 
   Size:        465     129
 
BsrFI r'CCGG_y

Cuts at:      0     523     594 
   Size:        523      71
 
BstBI TT'CG_AA

Cuts at:      0     285     594 
   Size:        285     309
 
BtgI C'CryG_G

Cuts at:      0      65     594 
   Size:         65     529
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      69     594 
   Size:         69     525
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      91     594 
   Size:         91     503
 
EcoICRI GAG'CTC

Cuts at:      0     126     594 
   Size:        126     468
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     594 
   Size:        114     480
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      70     102     594 
   Size:         70      32     492

  Fragments arranged by size: 

                 492      70      32
 
HindIII A'AGCT_T

Cuts at:      0     343     594 
   Size:        343     251
 
MspA1I CmG'CkG

Cuts at:      0      67     268     594 
   Size:         67     201     326

  Fragments arranged by size: 

                 326     201      67
 
NdeI CA'TA_TG

Cuts at:      0     328     594 
   Size:        328     266
 
PfoI T'CCnGG_A

Cuts at:      0      25     594 
   Size:         25     569
 
PstI C_TGCA'G

Cuts at:      0     491     594 
   Size:        491     103
 
PvuII CAG'CTG

Cuts at:      0     268     594 
   Size:        268     326
 
SacI G_AGCT'C

Cuts at:      0     128     594 
   Size:        128     466
 
SacII CC_GC'GG

Cuts at:      0      68     594 
   Size:         68     526
 
SfcI C'TryA_G

Cuts at:      0     487     498     594 
   Size:        487      11      96

  Fragments arranged by size: 

                 487      96      11
 
SmlI C'TyrA_G

Cuts at:      0      16     594 
   Size:         16     578
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     118     594 
   Size:        118     476
 

 Enzymes that do cut:

   AflIII     AgeI     ApoI     BaeI     BanI    BanII     BcgI    BmgBI
     BmrI    BpuEI    BsaWI    BseRI  BsiHKAI     BsmI Bsp1286I    BsrBI
    BsrFI    BstBI     BtgI     DrdI     EciI  EcoICRI EcoO109I    Hin4I
  HindIII   MspA1I     NdeI     PfoI     PstI    PvuII     SacI    SacII
     SfcI     SmlI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EarI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI