Restriction map for
pF1KE0638
(Linear) (Six-base) MAPSORT of: KIEE0638.seq Check: 6002 from: 1 to: 1341
KIEE0638.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 0 1166 1341
Size: 1166 175
AflIII A'CryG_T
Cuts at: 0 1058 1341
Size: 1058 283
AleI CACnn'nnGTG
Cuts at: 0 489 580 1341
Size: 489 91 761
Fragments arranged by size:
761 489 91
AlwNI CAG_nnn'CTG
Cuts at: 0 814 1341
Size: 814 527
ApaI G_GGCC'C
Cuts at: 0 1336 1341
Size: 1336 5
ApaLI G'TGCA_C
Cuts at: 0 1085 1341
Size: 1085 256
AscI GG'CGCG_CC
Cuts at: 0 1065 1341
Size: 1065 276
AvaI C'yCGr_G
Cuts at: 0 43 69 687 1080 1101 1341
Size: 43 26 618 393 21 240
Fragments arranged by size:
618 393 240 43 26 21
BamHI G'GATC_C
Cuts at: 0 943 1341
Size: 943 398
BanI G'GyrC_C
Cuts at: 0 75 367 558 1341
Size: 75 292 191 783
Fragments arranged by size:
783 292 191 75
BanII G_rGCy'C
Cuts at: 0 122 734 1245 1257 1336 1341
Size: 122 612 511 12 79 5
Fragments arranged by size:
612 511 122 79 12 5
BbeI G_GCGC'C
Cuts at: 0 79 1341
Size: 79 1262
BbsI GAAGACnn'nnnn_
Cuts at: 0 869 1341
Size: 869 472
BbvCI CC'TCA_GC
Cuts at: 0 109 1341
Size: 109 1232
BlpI GC'TnA_GC
Cuts at: 0 427 1132 1306 1341
Size: 427 705 174 35
Fragments arranged by size:
705 427 174 35
Bme1580I G_kGCm'C
Cuts at: 0 355 563 1089 1336 1341
Size: 355 208 526 247 5
Fragments arranged by size:
526 355 247 208 5
BmrI ACTGGGnnnn_n'
Cuts at: 0 623 1339 1341
Size: 623 716 2
Fragments arranged by size:
716 623 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 931 1341
Size: 469 462 410
Fragments arranged by size:
469 462 410
Bpu10I CC'TnA_GC
Cuts at: 0 109 1341
Size: 109 1232
BsaAI yAC'GTr
Cuts at: 0 533 882 1061 1341
Size: 533 349 179 280
Fragments arranged by size:
533 349 280 179
BsaHI Gr'CG_yC
Cuts at: 0 76 1341
Size: 76 1265
BsaWI w'CCGG_w
Cuts at: 0 20 37 544 1341
Size: 20 17 507 797
Fragments arranged by size:
797 507 20 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 268 1341
Size: 73 195 1073
Fragments arranged by size:
1073 195 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1341
Size: 49 1292
BsiHKAI G_wGCw'C
Cuts at: 0 734 1089 1098 1110 1245 1341
Size: 734 355 9 12 135 96
Fragments arranged by size:
734 355 135 96 12 9
BsmI GAATG_Cn'
Cuts at: 0 128 1341
Size: 128 1213
Bsp1286I G_dGCh'C
Cuts at: 0 122 355 563 734 1089 1098 1110 1245
Size: 122 233 208 171 355 9 12 135
Cuts at: 1245 1257 1336 1341
Size: 12 79 5
Fragments arranged by size:
355 233 208 171 135 122 79 12
12 9 5
BspEI T'CCGG_A
Cuts at: 0 544 1341
Size: 544 797
BsrBI CCG'CTC
Cuts at: 0 453 1341
Size: 453 888
BsrDI GCAATG_nn'
Cuts at: 0 1286 1341
Size: 1286 55
BsrFI r'CCGG_y
Cuts at: 0 836 1341
Size: 836 505
BsrGI T'GTAC_A
Cuts at: 0 572 1341
Size: 572 769
BssHII G'CGCG_C
Cuts at: 0 1065 1341
Size: 1065 276
BssSI C'ACGA_G
Cuts at: 0 146 433 485 599 1238 1341
Size: 146 287 52 114 639 103
Fragments arranged by size:
639 287 146 114 103 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 615 1341
Size: 615 726
BstXI CCAn_nnnn'nTGG
Cuts at: 0 348 447 975 1341
Size: 348 99 528 366
Fragments arranged by size:
528 366 348 99
BstYI r'GATC_y
Cuts at: 0 943 1341
Size: 943 398
Bsu36I CC'TnA_GG
Cuts at: 0 253 1341
Size: 253 1088
BtgI C'CryG_G
Cuts at: 0 704 1341
Size: 704 637
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 426 604 884 1341
Size: 426 178 280 457
Fragments arranged by size:
457 426 280 178
BtsI GCAGTG_nn'
Cuts at: 0 102 191 475 616 1341
Size: 102 89 284 141 725
Fragments arranged by size:
725 284 141 102 89
EaeI y'GGCC_r
Cuts at: 0 161 437 701 1341
Size: 161 276 264 640
Fragments arranged by size:
640 276 264 161
EarI CTCTTCn'nnn_
Cuts at: 0 1262 1341
Size: 1262 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 631 1341
Size: 631 710
EcoICRI GAG'CTC
Cuts at: 0 732 1243 1341
Size: 732 511 98
Fragments arranged by size:
732 511 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 931 1341
Size: 469 462 410
Fragments arranged by size:
469 462 410
EcoO109I rG'GnC_Cy
Cuts at: 0 595 1269 1333 1341
Size: 595 674 64 8
Fragments arranged by size:
674 595 64 8
HaeII r_GCGC'y
Cuts at: 0 79 464 925 1341
Size: 79 385 461 416
Fragments arranged by size:
461 416 385 79
HincII GTy'rAC
Cuts at: 0 1155 1341
Size: 1155 186
KasI G'GCGC_C
Cuts at: 0 75 1341
Size: 75 1266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 1341
Size: 186 1155
MscI TGG'CCA
Cuts at: 0 163 439 1341
Size: 163 276 902
Fragments arranged by size:
902 276 163
MslI CAynn'nnrTG
Cuts at: 0 226 403 489 580 1341
Size: 226 177 86 91 761
Fragments arranged by size:
761 226 177 91 86
MspA1I CmG'CkG
Cuts at: 0 113 1341
Size: 113 1228
NaeI GCC'GGC
Cuts at: 0 838 1341
Size: 838 503
NarI GG'CG_CC
Cuts at: 0 76 1341
Size: 76 1265
NgoMIV G'CCGG_C
Cuts at: 0 836 1341
Size: 836 505
NruI TCG'CGA
Cuts at: 0 728 1341
Size: 728 613
NspI r_CATG'y
Cuts at: 0 324 1341
Size: 324 1017
PasI CC'CwG_GG
Cuts at: 0 1250 1341
Size: 1250 91
PflMI CCAn_nnn'nTGG
Cuts at: 0 1061 1341
Size: 1061 280
PmlI CAC'GTG
Cuts at: 0 533 1061 1341
Size: 533 528 280
Fragments arranged by size:
533 528 280
PpuMI rG'GwC_Cy
Cuts at: 0 1269 1341
Size: 1269 72
PspOMI G'GGCC_C
Cuts at: 0 1332 1341
Size: 1332 9
PstI C_TGCA'G
Cuts at: 0 482 611 1341
Size: 482 129 730
Fragments arranged by size:
730 482 129
PvuII CAG'CTG
Cuts at: 0 113 1341
Size: 113 1228
SacI G_AGCT'C
Cuts at: 0 734 1245 1341
Size: 734 511 96
Fragments arranged by size:
734 511 96
SapI GCTCTTCn'nnn_
Cuts at: 0 1262 1341
Size: 1262 79
SexAI A'CCwGG_T
Cuts at: 0 649 1341
Size: 649 692
SfcI C'TryA_G
Cuts at: 0 478 607 1341
Size: 478 129 734
Fragments arranged by size:
734 478 129
SfoI GGC'GCC
Cuts at: 0 77 1341
Size: 77 1264
SmaI CCC'GGG
Cuts at: 0 45 71 689 1082 1341
Size: 45 26 618 393 259
Fragments arranged by size:
618 393 259 45 26
SphI G_CATG'C
Cuts at: 0 324 1341
Size: 324 1017
StuI AGG'CCT
Cuts at: 0 1198 1341
Size: 1198 143
StyI C'CwwG_G
Cuts at: 0 795 1341
Size: 795 546
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 976 1341
Size: 976 365
TatI w'GTAC_w
Cuts at: 0 572 1341
Size: 572 769
Tth111I GACn'n_nGTC
Cuts at: 0 651 1341
Size: 651 690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1326 1341
Size: 1326 15
XmaI C'CCGG_G
Cuts at: 0 43 69 687 1080 1341
Size: 43 26 618 393 261
Fragments arranged by size:
618 393 261 43 26
Enzymes that do cut:
AccI AflIII AleI AlwNI ApaI ApaLI AscI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BlpI Bme1580I
BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NruI NspI PasI PflMI PmlI PpuMI PspOMI
PstI PvuII SacI SapI SexAI SfcI SfoI SmaI
SphI StuI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApoI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BplI BpuEI BsaI BsaBI BsaXI BseYI BsiEI BsiWI
BsmBI BspHI BspMI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NsiI PacI PciI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TstI
XbaI XhoI XmnI ZraI