Restriction map for
pF1KE0639
(Linear) (Six-base) MAPSORT of: KIEE0639.seq Check: 3590 from: 1 to: 642
KIEE0639.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 642
Size: 433 209
AflIII A'CryG_T
Cuts at: 0 488 642
Size: 488 154
AhdI GACnn_n'nnGTC
Cuts at: 0 588 642
Size: 588 54
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 340 642
Size: 340 302
ApaI G_GGCC'C
Cuts at: 0 11 642
Size: 11 631
AvaI C'yCGr_G
Cuts at: 0 544 642
Size: 544 98
BanII G_rGCy'C
Cuts at: 0 11 439 642
Size: 11 428 203
Fragments arranged by size:
428 203 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 339 642
Size: 339 303
BglI GCCn_nnn'nGGC
Cuts at: 0 35 642
Size: 35 607
Bme1580I G_kGCm'C
Cuts at: 0 11 642
Size: 11 631
BmtI G_CTAG'C
Cuts at: 0 368 642
Size: 368 274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 642
Size: 181 461
BsaI GGTCTCn'nnnn_
Cuts at: 0 610 642
Size: 610 32
BsaAI yAC'GTr
Cuts at: 0 71 513 642
Size: 71 442 129
Fragments arranged by size:
442 129 71
BsaBI GATnn'nnATC
Cuts at: 0 190 642
Size: 190 452
BsaHI Gr'CG_yC
Cuts at: 0 572 642
Size: 572 70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 642
Size: 166 476
BseYI C'CCAG_C
Cuts at: 0 14 642
Size: 14 628
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 642
Size: 244 398
BsiHKAI G_wGCw'C
Cuts at: 0 439 518 642
Size: 439 79 124
Fragments arranged by size:
439 124 79
BsiWI C'GTAC_G
Cuts at: 0 71 642
Size: 71 571
BsmI GAATG_Cn'
Cuts at: 0 203 642
Size: 203 439
Bsp1286I G_dGCh'C
Cuts at: 0 11 439 518 642
Size: 11 428 79 124
Fragments arranged by size:
428 124 79 11
BsrGI T'GTAC_A
Cuts at: 0 83 642
Size: 83 559
BssSI C'ACGA_G
Cuts at: 0 110 642
Size: 110 532
BstAPI GCAn_nnn'nTGC
Cuts at: 0 381 642
Size: 381 261
BstYI r'GATC_y
Cuts at: 0 191 408 642
Size: 191 217 234
Fragments arranged by size:
234 217 191
BtgI C'CryG_G
Cuts at: 0 77 496 519 593 642
Size: 77 419 23 74 49
Fragments arranged by size:
419 77 74 49 23
DraIII CAC_nnn'GTG
Cuts at: 0 222 642
Size: 222 420
EcoICRI GAG'CTC
Cuts at: 0 437 642
Size: 437 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 642
Size: 433 209
EcoO109I rG'GnC_Cy
Cuts at: 0 7 19 642
Size: 7 12 623
Fragments arranged by size:
623 12 7
FspI TGC'GCA
Cuts at: 0 282 642
Size: 282 360
HaeII r_GCGC'y
Cuts at: 0 57 371 642
Size: 57 314 271
Fragments arranged by size:
314 271 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 574 606 642
Size: 574 32 36
Fragments arranged by size:
574 36 32
MspA1I CmG'CkG
Cuts at: 0 65 228 561 642
Size: 65 163 333 81
Fragments arranged by size:
333 163 81 65
NcoI C'CATG_G
Cuts at: 0 593 642
Size: 593 49
NheI G'CTAG_C
Cuts at: 0 364 642
Size: 364 278
NspI r_CATG'y
Cuts at: 0 492 642
Size: 492 150
PciI A'CATG_T
Cuts at: 0 488 642
Size: 488 154
PmlI CAC'GTG
Cuts at: 0 513 642
Size: 513 129
PpuMI rG'GwC_Cy
Cuts at: 0 19 642
Size: 19 623
PspOMI G'GGCC_C
Cuts at: 0 7 642
Size: 7 635
PvuII CAG'CTG
Cuts at: 0 228 642
Size: 228 414
SacI G_AGCT'C
Cuts at: 0 439 642
Size: 439 203
SanDI GG'GwC_CC
Cuts at: 0 19 642
Size: 19 623
SmlI C'TyrA_G
Cuts at: 0 196 642
Size: 196 446
StuI AGG'CCT
Cuts at: 0 91 642
Size: 91 551
StyI C'CwwG_G
Cuts at: 0 440 593 642
Size: 440 153 49
Fragments arranged by size:
440 153 49
TatI w'GTAC_w
Cuts at: 0 83 642
Size: 83 559
Enzymes that do cut:
AcuI AflIII AhdI AlfI ApaI AvaI BanII BbsI
BglI Bme1580I BmtI BpuEI BsaI BsaAI BsaBI BsaHI
BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BsrGI
BssSI BstAPI BstYI BtgI DraIII EcoICRI Eco57MI EcoO109I
FspI HaeII Hin4I MspA1I NcoI NheI NspI PciI
PmlI PpuMI PspOMI PvuII SacI SanDI SmlI StuI
StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BplI BpmI
Bpu10I BsaWI BsaXI BsiEI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI