(Linear) (Six-base) MAPSORT of: KIEE0641.seq  Check: 2670  from: 1  to: 651



KIEE0641.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AflII C'TTAA_G

Cuts at:      0     345     651 
   Size:        345     306
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     546     578     651 
   Size:        546      32      73

  Fragments arranged by size: 

                 546      73      32
 
BamHI G'GATC_C

Cuts at:      0     480     651 
   Size:        480     171
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     351     385     651 
   Size:        351      34     266

  Fragments arranged by size: 

                 351     266      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      51     651 
   Size:         51     600
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     554     651 
   Size:        554      97
 
BsaHI Gr'CG_yC

Cuts at:      0     597     651 
   Size:        597      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     651 
   Size:        173     478
 
BsiHKAI G_wGCw'C

Cuts at:      0      31     651 
   Size:         31     620
 
BsmI GAATG_Cn'

Cuts at:      0     514     651 
   Size:        514     137
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     651 
   Size:         31     620
 
BspHI T'CATG_A

Cuts at:      0     122     651 
   Size:        122     529
 
BsrDI GCAATG_nn'

Cuts at:      0     251     651 
   Size:        251     400
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     253     651 
   Size:        253     398
 
BstEII G'GTnAC_C

Cuts at:      0     114     651 
   Size:        114     537
 
BstYI r'GATC_y

Cuts at:      0     480     651 
   Size:        480     171
 
EarI CTCTTCn'nnn_

Cuts at:      0     139     264     651 
   Size:        139     125     387

  Fragments arranged by size: 

                 387     139     125
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     502     651 
   Size:        502     149
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     169     201     651 
   Size:        169      32     450

  Fragments arranged by size: 

                 450     169      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     651 
   Size:         68     583
 
MspA1I CmG'CkG

Cuts at:      0     277     651 
   Size:        277     374
 
PvuII CAG'CTG

Cuts at:      0     277     651 
   Size:        277     374
 
SapI GCTCTTCn'nnn_

Cuts at:      0     264     651 
   Size:        264     387
 
SmlI C'TyrA_G

Cuts at:      0     345     651 
   Size:        345     306
 
StyI C'CwwG_G

Cuts at:      0     601     651 
   Size:        601      50
 

 Enzymes that do cut:

    AflII     AloI    BamHI     BcgI    BciVI     BmrI    BsaHI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI   BstAPI   BstEII    BstYI
     EarI    EcoNI    Hin4I     MmeI   MspA1I    PvuII     SapI     SmlI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI