Restriction map for
pF1KE0644
(Linear) (Six-base) MAPSORT of: KIEE0644.seq Check: 9123 from: 1 to: 1293
KIEE0644.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 0 104 376 1293
Size: 104 272 917
Fragments arranged by size:
917 272 104
AhdI GACnn_n'nnGTC
Cuts at: 0 100 1293
Size: 100 1193
AleI CACnn'nnGTG
Cuts at: 0 154 537 1293
Size: 154 383 756
Fragments arranged by size:
756 383 154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 241 376 1293
Size: 241 135 917
Fragments arranged by size:
917 241 135
ApaLI G'TGCA_C
Cuts at: 0 625 1293
Size: 625 668
ApoI r'AATT_y
Cuts at: 0 1089 1269 1293
Size: 1089 180 24
Fragments arranged by size:
1089 180 24
BanI G'GyrC_C
Cuts at: 0 360 428 858 1293
Size: 360 68 430 435
Fragments arranged by size:
435 430 360 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 16 50 1293
Size: 16 34 1243
Fragments arranged by size:
1243 34 16
BfrBI ATG'CAT
Cuts at: 0 997 1293
Size: 997 296
BglI GCCn_nnn'nGGC
Cuts at: 0 136 1293
Size: 136 1157
BglII A'GATC_T
Cuts at: 0 635 1293
Size: 635 658
Bme1580I G_kGCm'C
Cuts at: 0 629 861 1293
Size: 629 232 432
Fragments arranged by size:
629 432 232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 48 1293
Size: 48 1245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1055 1293
Size: 295 760 238
Fragments arranged by size:
760 295 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 1293
Size: 577 716
BsaAI yAC'GTr
Cuts at: 0 204 546 1293
Size: 204 342 747
Fragments arranged by size:
747 342 204
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1053 1083 1293
Size: 1053 30 210
Fragments arranged by size:
1053 210 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1228 1293
Size: 1228 65
BseYI C'CCAG_C
Cuts at: 0 333 854 1293
Size: 333 521 439
Fragments arranged by size:
521 439 333
BsiEI CG_ry'CG
Cuts at: 0 97 1293
Size: 97 1196
BsiHKAI G_wGCw'C
Cuts at: 0 149 161 629 1293
Size: 149 12 468 664
Fragments arranged by size:
664 468 149 12
BsmI GAATG_Cn'
Cuts at: 0 293 1168 1293
Size: 293 875 125
Fragments arranged by size:
875 293 125
Bsp1286I G_dGCh'C
Cuts at: 0 149 161 629 861 1293
Size: 149 12 468 232 432
Fragments arranged by size:
468 432 232 149 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 281 418 1293
Size: 281 137 875
Fragments arranged by size:
875 281 137
BsrBI CCG'CTC
Cuts at: 0 99 1293
Size: 99 1194
BsrDI GCAATG_nn'
Cuts at: 0 192 413 1293
Size: 192 221 880
Fragments arranged by size:
880 221 192
BsrFI r'CCGG_y
Cuts at: 0 86 151 1293
Size: 86 65 1142
Fragments arranged by size:
1142 86 65
BsrGI T'GTAC_A
Cuts at: 0 698 1293
Size: 698 595
BssSI C'ACGA_G
Cuts at: 0 142 1293
Size: 142 1151
BstBI TT'CG_AA
Cuts at: 0 759 1293
Size: 759 534
BstEII G'GTnAC_C
Cuts at: 0 207 1293
Size: 207 1086
BstYI r'GATC_y
Cuts at: 0 635 1293
Size: 635 658
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 60 1293
Size: 60 1233
DraI TTT'AAA
Cuts at: 0 453 1293
Size: 453 840
EarI CTCTTCn'nnn_
Cuts at: 0 602 670 1293
Size: 602 68 623
Fragments arranged by size:
623 602 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1055 1293
Size: 295 760 238
Fragments arranged by size:
760 295 238
EcoRI G'AATT_C
Cuts at: 0 1269 1293
Size: 1269 24
FspI TGC'GCA
Cuts at: 0 229 1293
Size: 229 1064
FspAI rTGC'GCAy
Cuts at: 0 229 1293
Size: 229 1064
HaeII r_GCGC'y
Cuts at: 0 10 178 265 1293
Size: 10 168 87 1028
Fragments arranged by size:
1028 168 87 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 1293
Size: 86 32 1175
Fragments arranged by size:
1175 86 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 992 1293
Size: 347 645 301
Fragments arranged by size:
645 347 301
MslI CAynn'nnrTG
Cuts at: 0 154 418 537 820 1293
Size: 154 264 119 283 473
Fragments arranged by size:
473 283 264 154 119
MspA1I CmG'CkG
Cuts at: 0 382 1293
Size: 382 911
NdeI CA'TA_TG
Cuts at: 0 878 1293
Size: 878 415
NsiI A_TGCA'T
Cuts at: 0 999 1293
Size: 999 294
PmlI CAC'GTG
Cuts at: 0 546 1293
Size: 546 747
PstI C_TGCA'G
Cuts at: 0 1038 1293
Size: 1038 255
SfcI C'TryA_G
Cuts at: 0 1034 1293
Size: 1034 259
SgrAI Cr'CCGG_yG
Cuts at: 0 151 1293
Size: 151 1142
SmlI C'TyrA_G
Cuts at: 0 556 1293
Size: 556 737
StyI C'CwwG_G
Cuts at: 0 257 1293
Size: 257 1036
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 549 1293
Size: 549 744
TatI w'GTAC_w
Cuts at: 0 698 1293
Size: 698 595
Enzymes that do cut:
AccI AhdI AleI AlfI ApaLI ApoI BanI BcgI
BfrBI BglI BglII Bme1580I BplI BpmI BpuEI BsaAI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstYI
BtgZI DraI EarI Eco57MI EcoRI FspI FspAI HaeII
Hin4I MmeI MslI MspA1I NdeI NsiI PmlI PstI
SfcI SgrAI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BciVI
BclI BlpI BmgBI BmrI BmtI Bpu10I BsaI BsaBI
BsaHI BsaWI BsgI BsiWI BsmBI BspEI BspHI BssHII
BstAPI BstXI BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI