Restriction map for
pF1KE0646
(Linear) (Six-base) MAPSORT of: KIEE0646.seq Check: 5841 from: 1 to: 1140
KIEE0646.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 504 1140
Size: 504 636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 989 1140
Size: 49 940 151
Fragments arranged by size:
940 151 49
AfeI AGC'GCT
Cuts at: 0 70 1140
Size: 70 1070
AlwNI CAG_nnn'CTG
Cuts at: 0 472 840 1099 1140
Size: 472 368 259 41
Fragments arranged by size:
472 368 259 41
ApoI r'AATT_y
Cuts at: 0 673 1140
Size: 673 467
AvaI C'yCGr_G
Cuts at: 0 697 1140
Size: 697 443
BamHI G'GATC_C
Cuts at: 0 894 1140
Size: 894 246
BanI G'GyrC_C
Cuts at: 0 976 1134 1140
Size: 976 158 6
Fragments arranged by size:
976 158 6
BanII G_rGCy'C
Cuts at: 0 41 322 452 667 1063 1140
Size: 41 281 130 215 396 77
Fragments arranged by size:
396 281 215 130 77 41
BbeI G_GCGC'C
Cuts at: 0 980 1140
Size: 980 160
BbsI GAAGACnn'nnnn_
Cuts at: 0 851 1140
Size: 851 289
BlpI GC'TnA_GC
Cuts at: 0 421 1140
Size: 421 719
Bme1580I G_kGCm'C
Cuts at: 0 534 634 1137 1140
Size: 534 100 503 3
Fragments arranged by size:
534 503 100 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 498 1140
Size: 498 642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 1140
Size: 336 804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 323 331 802 817 1140
Size: 57 266 8 471 15 323
Fragments arranged by size:
471 323 266 57 15 8
BsaAI yAC'GTr
Cuts at: 0 635 1140
Size: 635 505
BsaBI GATnn'nnATC
Cuts at: 0 607 1140
Size: 607 533
BsaHI Gr'CG_yC
Cuts at: 0 977 1140
Size: 977 163
BsaWI w'CCGG_w
Cuts at: 0 414 1140
Size: 414 726
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 415 715 814 976 1117 1140
Size: 328 87 300 99 162 141 23
Fragments arranged by size:
328 300 162 141 99 87 23
BseYI C'CCAG_C
Cuts at: 0 106 193 213 460 556 639 724 1140
Size: 106 87 20 247 96 83 85 416
Fragments arranged by size:
416 247 106 96 87 85 83 20
BsiEI CG_ry'CG
Cuts at: 0 926 1140
Size: 926 214
BsiHKAI G_wGCw'C
Cuts at: 0 422 452 806 1140
Size: 422 30 354 334
Fragments arranged by size:
422 354 334 30
Bsp1286I G_dGCh'C
Cuts at: 0 41 322 422 452 534 634 667 806
Size: 41 281 100 30 82 100 33 139
Cuts at: 806 1063 1137 1140
Size: 257 74 3
Fragments arranged by size:
281 257 139 100 100 82 74 41
33 30 3
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 504 1140
Size: 504 636
BsrBI CCG'CTC
Cuts at: 0 1070 1140
Size: 1070 70
BssSI C'ACGA_G
Cuts at: 0 533 1140
Size: 533 607
BstAPI GCAn_nnn'nTGC
Cuts at: 0 777 1140
Size: 777 363
BstYI r'GATC_y
Cuts at: 0 894 1140
Size: 894 246
BtgI C'CryG_G
Cuts at: 0 116 707 1140
Size: 116 591 433
Fragments arranged by size:
591 433 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1091 1140
Size: 1091 49
BtsI GCAGTG_nn'
Cuts at: 0 239 450 963 1140
Size: 239 211 513 177
Fragments arranged by size:
513 239 211 177
DraIII CAC_nnn'GTG
Cuts at: 0 1011 1140
Size: 1011 129
DrdI GACnn_nn'nnGTC
Cuts at: 0 172 1140
Size: 172 968
EaeI y'GGCC_r
Cuts at: 0 525 923 1127 1140
Size: 525 398 204 13
Fragments arranged by size:
525 398 204 13
EagI C'GGCC_G
Cuts at: 0 923 1140
Size: 923 217
EarI CTCTTCn'nnn_
Cuts at: 0 193 287 965 1140
Size: 193 94 678 175
Fragments arranged by size:
678 193 175 94
EcoICRI GAG'CTC
Cuts at: 0 450 1140
Size: 450 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 57 323 331 802 817 989 1140
Size: 49 8 266 8 471 15 172 151
Fragments arranged by size:
471 266 172 151 49 15 8 8
EcoO109I rG'GnC_Cy
Cuts at: 0 282 514 1140
Size: 282 232 626
Fragments arranged by size:
626 282 232
HaeII r_GCGC'y
Cuts at: 0 72 382 980 1140
Size: 72 310 598 160
Fragments arranged by size:
598 310 160 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 587 591 619 793 825 1140
Size: 559 28 4 28 174 32 315
Fragments arranged by size:
559 315 174 32 28 28 4
KasI G'GCGC_C
Cuts at: 0 976 1140
Size: 976 164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 1140
Size: 995 145
MslI CAynn'nnrTG
Cuts at: 0 682 875 1140
Size: 682 193 265
Fragments arranged by size:
682 265 193
MspA1I CmG'CkG
Cuts at: 0 1110 1140
Size: 1110 30
NarI GG'CG_CC
Cuts at: 0 977 1140
Size: 977 163
NspI r_CATG'y
Cuts at: 0 718 1140
Size: 718 422
PflMI CCAn_nnn'nTGG
Cuts at: 0 472 840 1140
Size: 472 368 300
Fragments arranged by size:
472 368 300
PmlI CAC'GTG
Cuts at: 0 635 1140
Size: 635 505
PstI C_TGCA'G
Cuts at: 0 457 590 1140
Size: 457 133 550
Fragments arranged by size:
550 457 133
PvuII CAG'CTG
Cuts at: 0 1110 1140
Size: 1110 30
SacI G_AGCT'C
Cuts at: 0 452 1140
Size: 452 688
SfcI C'TryA_G
Cuts at: 0 345 453 586 1140
Size: 345 108 133 554
Fragments arranged by size:
554 345 133 108
SfoI GGC'GCC
Cuts at: 0 978 1140
Size: 978 162
SphI G_CATG'C
Cuts at: 0 718 1140
Size: 718 422
StuI AGG'CCT
Cuts at: 0 1118 1140
Size: 1118 22
StyI C'CwwG_G
Cuts at: 0 1022 1140
Size: 1022 118
TatI w'GTAC_w
Cuts at: 0 506 1140
Size: 506 634
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 1140
Size: 114 1026
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApoI AvaI BamHI BanI
BanII BbeI BbsI BlpI Bme1580I BmrI BplI BpmI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BssSI BstAPI BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NarI
NspI PflMI PmlI PstI PvuII SacI SfcI SfoI
SphI StuI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI Bpu10I BpuEI BsaI BsaXI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI ZraI