Restriction map for
pF1KE0650
(Linear) (Six-base) MAPSORT of: KIEE0650.seq Check: 9945 from: 1 to: 1146
KIEE0650.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 132 1146
Size: 132 1014
AccI GT'mk_AC
Cuts at: 0 254 1031 1146
Size: 254 777 115
Fragments arranged by size:
777 254 115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1081 1146
Size: 235 846 65
Fragments arranged by size:
846 235 65
AfeI AGC'GCT
Cuts at: 0 334 1146
Size: 334 812
AflII C'TTAA_G
Cuts at: 0 547 1146
Size: 547 599
AflIII A'CryG_T
Cuts at: 0 671 1146
Size: 671 475
AhdI GACnn_n'nnGTC
Cuts at: 0 675 1146
Size: 675 471
AlwNI CAG_nnn'CTG
Cuts at: 0 147 468 1146
Size: 147 321 678
Fragments arranged by size:
678 321 147
ApaI G_GGCC'C
Cuts at: 0 34 1146
Size: 34 1112
AvaI C'yCGr_G
Cuts at: 0 753 953 1146
Size: 753 200 193
Fragments arranged by size:
753 200 193
BanII G_rGCy'C
Cuts at: 0 34 101 1146
Size: 34 67 1045
Fragments arranged by size:
1045 67 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 862 1069 1146
Size: 862 207 77
Fragments arranged by size:
862 207 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 909 943 1146
Size: 909 34 203
Fragments arranged by size:
909 203 34
BfrBI ATG'CAT
Cuts at: 0 359 1146
Size: 359 787
BglI GCCn_nnn'nGGC
Cuts at: 0 788 1146
Size: 788 358
Bme1580I G_kGCm'C
Cuts at: 0 34 41 213 1146
Size: 34 7 172 933
Fragments arranged by size:
933 172 34 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 990 1146
Size: 990 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1146
Size: 196 950
Bpu10I CC'TnA_GC
Cuts at: 0 572 1146
Size: 572 574
BsaI GGTCTCn'nnnn_
Cuts at: 0 571 1146
Size: 571 575
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 331 780 1012 1072 1107 1146
Size: 34 297 449 232 60 35 39
Fragments arranged by size:
449 297 232 60 39 35 34
BseYI C'CCAG_C
Cuts at: 0 147 1146
Size: 147 999
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1146
Size: 454 692
BsiHKAI G_wGCw'C
Cuts at: 0 81 1146
Size: 81 1065
BsmI GAATG_Cn'
Cuts at: 0 361 1146
Size: 361 785
Bsp1286I G_dGCh'C
Cuts at: 0 34 41 81 101 213 1146
Size: 34 7 40 20 112 933
Fragments arranged by size:
933 112 40 34 20 7
BspHI T'CATG_A
Cuts at: 0 893 1146
Size: 893 253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 132 1146
Size: 132 1014
BsrDI GCAATG_nn'
Cuts at: 0 482 1146
Size: 482 664
BsrFI r'CCGG_y
Cuts at: 0 1076 1146
Size: 1076 70
BsrGI T'GTAC_A
Cuts at: 0 695 1146
Size: 695 451
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1014 1146
Size: 1014 132
Bsu36I CC'TnA_GG
Cuts at: 0 632 1146
Size: 632 514
BtgI C'CryG_G
Cuts at: 0 324 1146
Size: 324 822
BtsI GCAGTG_nn'
Cuts at: 0 119 452 548 1146
Size: 119 333 96 598
Fragments arranged by size:
598 333 119 96
ClaI AT'CG_AT
Cuts at: 0 919 1146
Size: 919 227
EaeI y'GGCC_r
Cuts at: 0 1016 1146
Size: 1016 130
EarI CTCTTCn'nnn_
Cuts at: 0 757 1146
Size: 757 389
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 832 1146
Size: 74 758 314
Fragments arranged by size:
758 314 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 235 1081 1146
Size: 196 39 846 65
Fragments arranged by size:
846 196 65 39
EcoO109I rG'GnC_Cy
Cuts at: 0 30 31 636 1056 1146
Size: 30 1 605 420 90
Fragments arranged by size:
605 420 90 30 1
HaeII r_GCGC'y
Cuts at: 0 336 1146
Size: 336 810
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 661 693 870 902 1146
Size: 661 32 177 32 244
Fragments arranged by size:
661 244 177 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1146
Size: 755 391
MscI TGG'CCA
Cuts at: 0 1018 1146
Size: 1018 128
MslI CAynn'nnrTG
Cuts at: 0 349 494 1146
Size: 349 145 652
Fragments arranged by size:
652 349 145
MspA1I CmG'CkG
Cuts at: 0 837 1146
Size: 837 309
NcoI C'CATG_G
Cuts at: 0 324 1146
Size: 324 822
NsiI A_TGCA'T
Cuts at: 0 361 1146
Size: 361 785
NspI r_CATG'y
Cuts at: 0 321 675 1146
Size: 321 354 471
Fragments arranged by size:
471 354 321
PasI CC'CwG_GG
Cuts at: 0 681 1146
Size: 681 465
PciI A'CATG_T
Cuts at: 0 671 1146
Size: 671 475
PflMI CCAn_nnn'nTGG
Cuts at: 0 921 1146
Size: 921 225
PfoI T'CCnGG_A
Cuts at: 0 299 1146
Size: 299 847
PpuMI rG'GwC_Cy
Cuts at: 0 636 1056 1146
Size: 636 420 90
Fragments arranged by size:
636 420 90
PspOMI G'GGCC_C
Cuts at: 0 30 1146
Size: 30 1116
PstI C_TGCA'G
Cuts at: 0 432 599 965 1146
Size: 432 167 366 181
Fragments arranged by size:
432 366 181 167
PvuII CAG'CTG
Cuts at: 0 837 1146
Size: 837 309
SexAI A'CCwGG_T
Cuts at: 0 1040 1146
Size: 1040 106
SfcI C'TryA_G
Cuts at: 0 428 595 961 1146
Size: 428 167 366 185
Fragments arranged by size:
428 366 185 167
SmaI CCC'GGG
Cuts at: 0 755 1146
Size: 755 391
SmlI C'TyrA_G
Cuts at: 0 547 1146
Size: 547 599
StyI C'CwwG_G
Cuts at: 0 236 324 1013 1146
Size: 236 88 689 133
Fragments arranged by size:
689 236 133 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 145 598 673 1146
Size: 133 12 453 75 473
Fragments arranged by size:
473 453 133 75 12
TatI w'GTAC_w
Cuts at: 0 695 1146
Size: 695 451
Tth111I GACn'n_nGTC
Cuts at: 0 988 1146
Size: 988 158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 193 1146
Size: 144 49 953
Fragments arranged by size:
953 144 49
XmaI C'CCGG_G
Cuts at: 0 753 1146
Size: 753 393
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AhdI AlwNI
ApaI AvaI BanII BbsI BcgI BfrBI BglI Bme1580I
BmrI BpmI Bpu10I BsaI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstXI
Bsu36I BtgI BtsI ClaI EaeI EarI EciI Eco57MI
EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PciI PflMI PfoI PpuMI PspOMI
PstI PvuII SexAI SfcI SmaI SmlI StyI TaqII
TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AleI AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BciVI BclI BglII BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BsrBI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgZI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XhoI XmnI ZraI