(Linear) (Six-base) MAPSORT of: KIEE0654.seq  Check: 8459  from: 1  to: 1158



KIEE0654.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:      0     647    1158 
   Size:        647     511
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377    1158 
   Size:        377     781
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     188    1004    1126    1158 
   Size:        188     816     122      32

  Fragments arranged by size: 

                 816     188     122      32
 
ApoI r'AATT_y

Cuts at:      0     725    1158 
   Size:        725     433
 
AvaI C'yCGr_G

Cuts at:      0     404    1158 
   Size:        404     754
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     528    1037    1158 
   Size:        528     509     121

  Fragments arranged by size: 

                 528     509     121
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     114    1158 
   Size:        114    1044
 
BglI GCCn_nnn'nGGC

Cuts at:      0     926    1092    1158 
   Size:        926     166      66

  Fragments arranged by size: 

                 926     166      66
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     549    1158 
   Size:        549     609
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1073    1158 
   Size:       1073      85
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     803    1034    1158 
   Size:        803     231     124

  Fragments arranged by size: 

                 803     231     124
 
BspHI T'CATG_A

Cuts at:      0     159    1158 
   Size:        159     999
 
BsrBI CCG'CTC

Cuts at:      0     562    1158 
   Size:        562     596
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     190     790    1158 
   Size:        190     600     368

  Fragments arranged by size: 

                 600     368     190
 
BstEII G'GTnAC_C

Cuts at:      0      34    1158 
   Size:         34    1124
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     514     916     937    1158 
   Size:        514     402      21     221

  Fragments arranged by size: 

                 514     402     221      21
 
BstZ17I GTA'TAC

Cuts at:      0     648    1158 
   Size:        648     510
 
BtgI C'CryG_G

Cuts at:      0    1054    1158 
   Size:       1054     104
 
EaeI y'GGCC_r

Cuts at:      0     504     918     957    1005    1158 
   Size:        504     414      39      48     153

  Fragments arranged by size: 

                 504     414     153      48      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377    1158 
   Size:        377     781
 
EcoRV GAT'ATC

Cuts at:      0     904    1158 
   Size:        904     254
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     877    1158 
   Size:        877     281
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      45      77    1158 
   Size:         45      32    1081

  Fragments arranged by size: 

                1081      45      32
 
MfeI C'AATT_G

Cuts at:      0     794    1158 
   Size:        794     364
 
MscI TGG'CCA

Cuts at:      0     506     920     959    1007    1158 
   Size:        506     414      39      48     151

  Fragments arranged by size: 

                 506     414     151      48      39
 
MslI CAynn'nnrTG

Cuts at:      0     170     317     779    1158 
   Size:        170     147     462     379

  Fragments arranged by size: 

                 462     379     170     147
 
MspA1I CmG'CkG

Cuts at:      0     127     178    1158 
   Size:        127      51     980

  Fragments arranged by size: 

                 980     127      51
 
NcoI C'CATG_G

Cuts at:      0    1054    1158 
   Size:       1054     104
 
NdeI CA'TA_TG

Cuts at:      0     576     603     993    1158 
   Size:        576      27     390     165

  Fragments arranged by size: 

                 576     390     165      27
 
NspI r_CATG'y

Cuts at:      0     793    1110    1158 
   Size:        793     317      48

  Fragments arranged by size: 

                 793     317      48
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1072    1158 
   Size:       1072      86
 
PstI C_TGCA'G

Cuts at:      0     183    1158 
   Size:        183     975
 
PvuII CAG'CTG

Cuts at:      0     127     178    1158 
   Size:        127      51     980

  Fragments arranged by size: 

                 980     127      51
 
SfcI C'TryA_G

Cuts at:      0     179    1158 
   Size:        179     979
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     926    1158 
   Size:        926     232
 
SmlI C'TyrA_G

Cuts at:      0    1088    1158 
   Size:       1088      70
 
StyI C'CwwG_G

Cuts at:      0    1054    1158 
   Size:       1054     104
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     490    1158 
   Size:        490     668
 
TatI w'GTAC_w

Cuts at:      0     571     640    1158 
   Size:        571      69     518

  Fragments arranged by size: 

                 571     518      69
 
Tth111I GACn'n_nGTC

Cuts at:      0    1041    1158 
   Size:       1041     117
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     325     495     514    1158 
   Size:        325     170      19     644

  Fragments arranged by size: 

                 644     325     170      19
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     ApoI     AvaI     BbsI    BciVI     BglI
     BmrI    BpuEI     BsgI    BspHI    BsrBI   BstAPI   BstEII    BstXI
  BstZ17I     BtgI     EaeI  Eco57MI    EcoRV     FalI    Hin4I     MfeI
     MscI     MslI   MspA1I     NcoI     NdeI     NspI    PflMI     PstI
    PvuII     SfcI     SfiI     SmlI     StyI    TaqII     TatI  Tth111I
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI    BstYI   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI     XhoI
     XmaI     XmnI     ZraI