(Linear) (Six-base) MAPSORT of: KIEE0660.seq  Check: 2838  from: 1  to: 1167



KIEE0660.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     234     768    1104    1167 
   Size:         93     141     534     336      63

  Fragments arranged by size: 

                 534     336     141      93      63
 
AleI CACnn'nnGTG

Cuts at:      0     997    1167 
   Size:        997     170
 
ApoI r'AATT_y

Cuts at:      0     264    1167 
   Size:        264     903
 
BamHI G'GATC_C

Cuts at:      0      63    1167 
   Size:         63    1104
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     149     324    1167 
   Size:        149     175     843

  Fragments arranged by size: 

                 843     175     149
 
BfrBI ATG'CAT

Cuts at:      0     676    1167 
   Size:        676     491
 
BglII A'GATC_T

Cuts at:      0     733    1167 
   Size:        733     434
 
Bme1580I G_kGCm'C

Cuts at:      0    1053    1167 
   Size:       1053     114
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     596     680     764     848    1016    1100    1167 
   Size:        314     282      84      84      84     168      84      67

  Fragments arranged by size: 

                 314     282     168      84      84      84      84      67
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61    1167 
   Size:         61    1106
 
BsiHKAI G_wGCw'C

Cuts at:      0     568    1167 
   Size:        568     599
 
BsmI GAATG_Cn'

Cuts at:      0     316    1167 
   Size:        316     851
 
Bsp1286I G_dGCh'C

Cuts at:      0     568    1053    1167 
   Size:        568     485     114

  Fragments arranged by size: 

                 568     485     114
 
BsrDI GCAATG_nn'

Cuts at:      0     423    1167 
   Size:        423     744
 
BstBI TT'CG_AA

Cuts at:      0    1056    1140    1167 
   Size:       1056      84      27

  Fragments arranged by size: 

                1056      84      27
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     801    1167 
   Size:        801     366
 
BstYI r'GATC_y

Cuts at:      0      63     733    1167 
   Size:         63     670     434

  Fragments arranged by size: 

                 670     434      63
 
BtsI GCAGTG_nn'

Cuts at:      0     321    1167 
   Size:        321     846
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     417     452    1167 
   Size:        417      35     715

  Fragments arranged by size: 

                 715     417      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     722    1167 
   Size:        722     445
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     234     314     596     680     764     768     848 
   Size:         93     141      80     282      84      84       4      80

Cuts at:    848    1016    1100    1104    1167 
   Size:        168      84       4      63

  Fragments arranged by size: 

                 282     168     141      93      84      84      84      80
                  80      63       4       4
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27    1167 
   Size:         27    1140
 
HincII GTy'rAC

Cuts at:      0     468    1167 
   Size:        468     699
 
MslI CAynn'nnrTG

Cuts at:      0     309     591     997    1167 
   Size:        309     282     406     170

  Fragments arranged by size: 

                 406     309     282     170
 
NdeI CA'TA_TG

Cuts at:      0     337    1167 
   Size:        337     830
 
NsiI A_TGCA'T

Cuts at:      0     678    1167 
   Size:        678     489
 
NspI r_CATG'y

Cuts at:      0     680    1167 
   Size:        680     487
 
PsiI TTA'TAA

Cuts at:      0     506    1167 
   Size:        506     661
 
SfcI C'TryA_G

Cuts at:      0      84     125    1167 
   Size:         84      41    1042

  Fragments arranged by size: 

                1042      84      41
 
SpeI A'CTAG_T

Cuts at:      0     490    1167 
   Size:        490     677
 
SspI AAT'ATT

Cuts at:      0     958    1167 
   Size:        958     209
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     894     926    1167 
   Size:        894      32     241

  Fragments arranged by size: 

                 894     241      32
 

 Enzymes that do cut:

     AcuI     AleI     ApoI    BamHI     BbsI    BfrBI    BglII Bme1580I
     BpmI    BseRI  BsiHKAI     BsmI Bsp1286I    BsrDI    BstBI    BstXI
    BstYI     BtsI    CspCI     EarI  Eco57MI    Hin4I   HincII     MslI
     NdeI     NsiI     NspI     PsiI     SfcI     SpeI     SspI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI