Restriction map for
pF1KE0668
(Linear) (Six-base) MAPSORT of: KIEE0668.seq Check: 2157 from: 1 to: 1179
KIEE0668.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 484 1179
Size: 208 276 695
Fragments arranged by size:
695 276 208
AflIII A'CryG_T
Cuts at: 0 686 1179
Size: 686 493
AlwNI CAG_nnn'CTG
Cuts at: 0 351 422 1179
Size: 351 71 757
Fragments arranged by size:
757 351 71
ApoI r'AATT_y
Cuts at: 0 479 491 997 1179
Size: 479 12 506 182
Fragments arranged by size:
506 479 182 12
AvaI C'yCGr_G
Cuts at: 0 877 1179
Size: 877 302
BanI G'GyrC_C
Cuts at: 0 827 1179
Size: 827 352
BbvCI CC'TCA_GC
Cuts at: 0 1097 1179
Size: 1097 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 68 1179
Size: 34 34 1111
Fragments arranged by size:
1111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 791 1179
Size: 791 388
BglII A'GATC_T
Cuts at: 0 1087 1179
Size: 1087 92
Bme1580I G_kGCm'C
Cuts at: 0 676 1179
Size: 676 503
BmrI ACTGGGnnnn_n'
Cuts at: 0 971 1179
Size: 971 208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 381 1179
Size: 381 798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 322 688 759 779 832 1179
Size: 130 192 366 71 20 53 347
Fragments arranged by size:
366 347 192 130 71 53 20
Bpu10I CC'TnA_GC
Cuts at: 0 889 1097 1179
Size: 889 208 82
Fragments arranged by size:
889 208 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1179
Size: 169 1010
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 258 847 1179
Size: 73 185 589 332
Fragments arranged by size:
589 332 185 73
BsaBI GATnn'nnATC
Cuts at: 0 418 1179
Size: 418 761
BsaHI Gr'CG_yC
Cuts at: 0 19 1175 1179
Size: 19 1156 4
Fragments arranged by size:
1156 19 4
BsaWI w'CCGG_w
Cuts at: 0 320 1179
Size: 320 859
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 805 835 1179
Size: 805 30 344
Fragments arranged by size:
805 344 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 782 953 1179
Size: 127 655 171 226
Fragments arranged by size:
655 226 171 127
BseYI C'CCAG_C
Cuts at: 0 219 1150 1179
Size: 219 931 29
Fragments arranged by size:
931 219 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1179
Size: 745 434
BsiHKAI G_wGCw'C
Cuts at: 0 434 1179
Size: 434 745
Bsp1286I G_dGCh'C
Cuts at: 0 434 676 1179
Size: 434 242 503
Fragments arranged by size:
503 434 242
BspEI T'CCGG_A
Cuts at: 0 320 1179
Size: 320 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 336 1105 1179
Size: 336 769 74
Fragments arranged by size:
769 336 74
BsrDI GCAATG_nn'
Cuts at: 0 237 509 1179
Size: 237 272 670
Fragments arranged by size:
670 272 237
BstAPI GCAn_nnn'nTGC
Cuts at: 0 351 1179
Size: 351 828
BstXI CCAn_nnnn'nTGG
Cuts at: 0 810 1171 1179
Size: 810 361 8
Fragments arranged by size:
810 361 8
BstYI r'GATC_y
Cuts at: 0 1087 1179
Size: 1087 92
BtgI C'CryG_G
Cuts at: 0 600 653 920 1179
Size: 600 53 267 259
Fragments arranged by size:
600 267 259 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 419 1179
Size: 419 760
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 582 617 1179
Size: 582 35 562
Fragments arranged by size:
582 562 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 628 1179
Size: 628 551
EaeI y'GGCC_r
Cuts at: 0 281 656 1179
Size: 281 375 523
Fragments arranged by size:
523 375 281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 208 322 484 688 759 779 832
Size: 130 78 114 162 204 71 20 53
Cuts at: 832 1179
Size: 347
Fragments arranged by size:
347 204 162 130 114 78 71 53
20
EcoO109I rG'GnC_Cy
Cuts at: 0 680 872 884 1179
Size: 680 192 12 295
Fragments arranged by size:
680 295 192 12
EcoRI G'AATT_C
Cuts at: 0 479 1179
Size: 479 700
HaeII r_GCGC'y
Cuts at: 0 147 1179
Size: 147 1032
HincII GTy'rAC
Cuts at: 0 60 594 954 1179
Size: 60 534 360 225
Fragments arranged by size:
534 360 225 60
HpaI GTT'AAC
Cuts at: 0 60 1179
Size: 60 1119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 920 1179
Size: 605 315 259
Fragments arranged by size:
605 315 259
MscI TGG'CCA
Cuts at: 0 283 658 1179
Size: 283 375 521
Fragments arranged by size:
521 375 283
MslI CAynn'nnrTG
Cuts at: 0 437 566 1179
Size: 437 129 613
Fragments arranged by size:
613 437 129
MspA1I CmG'CkG
Cuts at: 0 30 635 1179
Size: 30 605 544
Fragments arranged by size:
605 544 30
NcoI C'CATG_G
Cuts at: 0 653 920 1179
Size: 653 267 259
Fragments arranged by size:
653 267 259
NspI r_CATG'y
Cuts at: 0 690 1179
Size: 690 489
PciI A'CATG_T
Cuts at: 0 686 1179
Size: 686 493
PpuMI rG'GwC_Cy
Cuts at: 0 680 872 1179
Size: 680 192 307
Fragments arranged by size:
680 307 192
PstI C_TGCA'G
Cuts at: 0 57 347 1179
Size: 57 290 832
Fragments arranged by size:
832 290 57
PvuII CAG'CTG
Cuts at: 0 30 1179
Size: 30 1149
SfcI C'TryA_G
Cuts at: 0 53 343 399 1179
Size: 53 290 56 780
Fragments arranged by size:
780 290 56 53
SmaI CCC'GGG
Cuts at: 0 879 1179
Size: 879 300
SmlI C'TyrA_G
Cuts at: 0 148 1179
Size: 148 1031
StuI AGG'CCT
Cuts at: 0 94 154 527 1179
Size: 94 60 373 652
Fragments arranged by size:
652 373 94 60
StyI C'CwwG_G
Cuts at: 0 653 803 868 920 1179
Size: 653 150 65 52 259
Fragments arranged by size:
653 259 150 65 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 815 876 1179
Size: 815 61 303
Fragments arranged by size:
815 303 61
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 265 666 1179
Size: 265 401 513
Fragments arranged by size:
513 401 265
XmaI C'CCGG_G
Cuts at: 0 877 1179
Size: 877 302
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AvaI BanI BbvCI BcgI
BciVI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrDI BstAPI BstXI BstYI
BtgI BtgZI CspCI DrdI EaeI Eco57MI EcoO109I EcoRI
HaeII HincII HpaI MmeI MscI MslI MspA1I NcoI
NspI PciI PpuMI PstI PvuII SfcI SmaI SmlI
StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbsI BclI
BfrBI BglI BlpI BmgBI BmtI BsaAI BsiEI BsiWI
BsmI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraI DraIII
EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI Hin4I HindIII KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmnI ZraI