Restriction map for
pF1KE0669
(Linear) (Six-base) MAPSORT of: KIEE0669.seq Check: 9791 from: 1 to: 747
KIEE0669.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 747
Size: 573 174
AleI CACnn'nnGTG
Cuts at: 0 271 747
Size: 271 476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 289 427 747
Size: 289 138 320
Fragments arranged by size:
320 289 138
AlwNI CAG_nnn'CTG
Cuts at: 0 378 435 747
Size: 378 57 312
Fragments arranged by size:
378 312 57
ApaI G_GGCC'C
Cuts at: 0 653 711 747
Size: 653 58 36
Fragments arranged by size:
653 58 36
AvaI C'yCGr_G
Cuts at: 0 257 601 747
Size: 257 344 146
Fragments arranged by size:
344 257 146
BanI G'GyrC_C
Cuts at: 0 639 747
Size: 639 108
BanII G_rGCy'C
Cuts at: 0 170 608 653 711 747
Size: 170 438 45 58 36
Fragments arranged by size:
438 170 58 45 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 170 747
Size: 136 34 577
Fragments arranged by size:
577 136 34
BglI GCCn_nnn'nGGC
Cuts at: 0 528 747
Size: 528 219
BlpI GC'TnA_GC
Cuts at: 0 727 747
Size: 727 20
Bme1580I G_kGCm'C
Cuts at: 0 540 653 711 747
Size: 540 113 58 36
Fragments arranged by size:
540 113 58 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 423 466 527 747
Size: 423 43 61 220
Fragments arranged by size:
423 220 61 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 388 591 747
Size: 208 180 203 156
Fragments arranged by size:
208 203 180 156
BsaAI yAC'GTr
Cuts at: 0 273 391 747
Size: 273 118 356
Fragments arranged by size:
356 273 118
BsaHI Gr'CG_yC
Cuts at: 0 44 176 747
Size: 44 132 571
Fragments arranged by size:
571 132 44
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 747
Size: 238 509
BseYI C'CCAG_C
Cuts at: 0 308 416 422 495 507 747
Size: 308 108 6 73 12 240
Fragments arranged by size:
308 240 108 73 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 664 747
Size: 180 484 83
Fragments arranged by size:
484 180 83
BsiHKAI G_wGCw'C
Cuts at: 0 577 747
Size: 577 170
Bsp1286I G_dGCh'C
Cuts at: 0 170 540 577 608 653 711 747
Size: 170 370 37 31 45 58 36
Fragments arranged by size:
370 170 58 45 37 36 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 747
Size: 353 394
BsrFI r'CCGG_y
Cuts at: 0 104 527 747
Size: 104 423 220
Fragments arranged by size:
423 220 104
BsrGI T'GTAC_A
Cuts at: 0 71 747
Size: 71 676
BssHII G'CGCG_C
Cuts at: 0 262 747
Size: 262 485
BssSI C'ACGA_G
Cuts at: 0 682 747
Size: 682 65
BstYI r'GATC_y
Cuts at: 0 701 747
Size: 701 46
BtgI C'CryG_G
Cuts at: 0 170 299 645 747
Size: 170 129 346 102
Fragments arranged by size:
346 170 129 102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 63 98 747
Size: 63 35 649
Fragments arranged by size:
649 63 35
EaeI y'GGCC_r
Cuts at: 0 278 693 717 747
Size: 278 415 24 30
Fragments arranged by size:
415 278 30 24
EarI CTCTTCn'nnn_
Cuts at: 0 142 747
Size: 142 605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 388 573 591 747
Size: 208 180 185 18 156
Fragments arranged by size:
208 185 180 156 18
EcoO109I rG'GnC_Cy
Cuts at: 0 254 318 650 708 747
Size: 254 64 332 58 39
Fragments arranged by size:
332 254 64 58 39
HaeII r_GCGC'y
Cuts at: 0 111 747
Size: 111 636
MscI TGG'CCA
Cuts at: 0 695 719 747
Size: 695 24 28
Fragments arranged by size:
695 28 24
MslI CAynn'nnrTG
Cuts at: 0 271 747
Size: 271 476
MspA1I CmG'CkG
Cuts at: 0 283 378 747
Size: 283 95 369
Fragments arranged by size:
369 283 95
NaeI GCC'GGC
Cuts at: 0 529 747
Size: 529 218
NcoI C'CATG_G
Cuts at: 0 299 747
Size: 299 448
NgoMIV G'CCGG_C
Cuts at: 0 527 747
Size: 527 220
NspI r_CATG'y
Cuts at: 0 135 747
Size: 135 612
PfoI T'CCnGG_A
Cuts at: 0 185 747
Size: 185 562
PpuMI rG'GwC_Cy
Cuts at: 0 254 747
Size: 254 493
PspOMI G'GGCC_C
Cuts at: 0 649 707 747
Size: 649 58 40
Fragments arranged by size:
649 58 40
PvuII CAG'CTG
Cuts at: 0 378 747
Size: 378 369
SanDI GG'GwC_CC
Cuts at: 0 254 747
Size: 254 493
SapI GCTCTTCn'nnn_
Cuts at: 0 142 747
Size: 142 605
SgrAI Cr'CCGG_yG
Cuts at: 0 104 747
Size: 104 643
SmaI CCC'GGG
Cuts at: 0 259 747
Size: 259 488
StuI AGG'CCT
Cuts at: 0 594 747
Size: 594 153
StyI C'CwwG_G
Cuts at: 0 299 548 747
Size: 299 249 199
Fragments arranged by size:
299 249 199
TatI w'GTAC_w
Cuts at: 0 71 747
Size: 71 676
XmaI C'CCGG_G
Cuts at: 0 257 747
Size: 257 490
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI AvaI BanI BanII
BcgI BglI BlpI Bme1580I BmrI BpmI BsaAI BsaHI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BsrGI
BssHII BssSI BstYI BtgI CspCI EaeI EarI Eco57MI
EcoO109I HaeII MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PfoI PpuMI PspOMI PvuII SanDI SapI SgrAI
SmaI StuI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmnI ZraI