Restriction map for
pF1KE0671
(Linear) (Six-base) MAPSORT of: KIEE0671.seq Check: 1093 from: 1 to: 774
KIEE0671.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 293 774
Size: 293 481
Acc65I G'GTAC_C
Cuts at: 0 145 774
Size: 145 629
AlwNI CAG_nnn'CTG
Cuts at: 0 610 774
Size: 610 164
ApaI G_GGCC'C
Cuts at: 0 72 774
Size: 72 702
AvaI C'yCGr_G
Cuts at: 0 127 774
Size: 127 647
BanI G'GyrC_C
Cuts at: 0 145 774
Size: 145 629
BanII G_rGCy'C
Cuts at: 0 72 774
Size: 72 702
BglI GCCn_nnn'nGGC
Cuts at: 0 271 774
Size: 271 503
Bme1580I G_kGCm'C
Cuts at: 0 72 660 774
Size: 72 588 114
Fragments arranged by size:
588 114 72
BmgBI CAC'GTC
Cuts at: 0 711 774
Size: 711 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 59 774
Size: 59 715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 774
Size: 390 384
BsaAI yAC'GTr
Cuts at: 0 319 774
Size: 319 455
BsaWI w'CCGG_w
Cuts at: 0 459 774
Size: 459 315
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 205 587 774
Size: 145 60 382 187
Fragments arranged by size:
382 187 145 60
BseYI C'CCAG_C
Cuts at: 0 115 150 334 582 774
Size: 115 35 184 248 192
Fragments arranged by size:
248 192 184 115 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 499 652 774
Size: 483 16 153 122
Fragments arranged by size:
483 153 122 16
Bsp1286I G_dGCh'C
Cuts at: 0 72 660 774
Size: 72 588 114
Fragments arranged by size:
588 114 72
BspEI T'CCGG_A
Cuts at: 0 459 774
Size: 459 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 774
Size: 293 481
BsrBI CCG'CTC
Cuts at: 0 45 774
Size: 45 729
BsrGI T'GTAC_A
Cuts at: 0 615 774
Size: 615 159
BssSI C'ACGA_G
Cuts at: 0 368 774
Size: 368 406
BstAPI GCAn_nnn'nTGC
Cuts at: 0 108 294 592 774
Size: 108 186 298 182
Fragments arranged by size:
298 186 182 108
BstYI r'GATC_y
Cuts at: 0 195 774
Size: 195 579
Bsu36I CC'TnA_GG
Cuts at: 0 560 774
Size: 560 214
BtsI GCAGTG_nn'
Cuts at: 0 500 586 608 660 724 774
Size: 500 86 22 52 64 50
Fragments arranged by size:
500 86 64 52 50 22
DraIII CAC_nnn'GTG
Cuts at: 0 489 774
Size: 489 285
EaeI y'GGCC_r
Cuts at: 0 263 704 740 774
Size: 263 441 36 34
Fragments arranged by size:
441 263 36 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 774
Size: 390 384
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 774
Size: 130 644
EcoO109I rG'GnC_Cy
Cuts at: 0 19 69 119 774
Size: 19 50 50 655
Fragments arranged by size:
655 50 50 19
KpnI G_GTAC'C
Cuts at: 0 149 774
Size: 149 625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 774
Size: 449 325
MspA1I CmG'CkG
Cuts at: 0 57 774
Size: 57 717
PpuMI rG'GwC_Cy
Cuts at: 0 19 774
Size: 19 755
PspOMI G'GGCC_C
Cuts at: 0 68 774
Size: 68 706
PspXI vC'TCGA_Gb
Cuts at: 0 127 774
Size: 127 647
PstI C_TGCA'G
Cuts at: 0 290 774
Size: 290 484
SfcI C'TryA_G
Cuts at: 0 286 774
Size: 286 488
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 271 774
Size: 271 503
SmlI C'TyrA_G
Cuts at: 0 127 774
Size: 127 647
TatI w'GTAC_w
Cuts at: 0 615 774
Size: 615 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 187 219 774
Size: 187 32 555
Fragments arranged by size:
555 187 32
Tth111I GACn'n_nGTC
Cuts at: 0 23 774
Size: 23 751
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 774
Size: 414 360
XhoI C'TCGA_G
Cuts at: 0 127 774
Size: 127 647
Enzymes that do cut:
AarI Acc65I AlwNI ApaI AvaI BanI BanII BglI
Bme1580I BmgBI BmrI BpmI BsaAI BsaWI BseRI BseYI
BsgI Bsp1286I BspEI BspMI BsrBI BsrGI BssSI BstAPI
BstYI Bsu36I BtsI DraIII EaeI Eco57MI EcoNI EcoO109I
KpnI MmeI MspA1I PpuMI PspOMI PspXI PstI SfcI
SfiI SmlI TatI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspHI BsrDI BsrFI BssHII BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DrdI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MluI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII XbaI XmaI XmnI ZraI