Restriction map for
pF1KE0672
(Linear) (Six-base) MAPSORT of: KIEE0672.seq Check: 6221 from: 1 to: 1029
KIEE0672.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 787 1029
Size: 787 242
AccI GT'mk_AC
Cuts at: 0 41 1029
Size: 41 988
Acc65I G'GTAC_C
Cuts at: 0 623 1029
Size: 623 406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 645 1029
Size: 31 614 384
Fragments arranged by size:
614 384 31
AlwNI CAG_nnn'CTG
Cuts at: 0 672 1029
Size: 672 357
ApaI G_GGCC'C
Cuts at: 0 591 1029
Size: 591 438
ApaLI G'TGCA_C
Cuts at: 0 397 1029
Size: 397 632
AvaI C'yCGr_G
Cuts at: 0 66 1029
Size: 66 963
BanI G'GyrC_C
Cuts at: 0 70 364 508 623 743 797 1029
Size: 70 294 144 115 120 54 232
Fragments arranged by size:
294 232 144 120 115 70 54
BanII G_rGCy'C
Cuts at: 0 109 247 548 591 659 1029
Size: 109 138 301 43 68 370
Fragments arranged by size:
370 301 138 109 68 43
BbeI G_GCGC'C
Cuts at: 0 368 1029
Size: 368 661
BglI GCCn_nnn'nGGC
Cuts at: 0 998 1029
Size: 998 31
Bme1580I G_kGCm'C
Cuts at: 0 401 591 1029
Size: 401 190 438
Fragments arranged by size:
438 401 190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 333 711 981 1029
Size: 123 210 378 270 48
Fragments arranged by size:
378 270 210 123 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 772 1029
Size: 247 525 257
Fragments arranged by size:
525 257 247
Bpu10I CC'TnA_GC
Cuts at: 0 653 1029
Size: 653 376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 394 1029
Size: 112 282 635
Fragments arranged by size:
635 282 112
BsaI GGTCTCn'nnnn_
Cuts at: 0 468 752 1029
Size: 468 284 277
Fragments arranged by size:
468 284 277
BsaHI Gr'CG_yC
Cuts at: 0 365 596 1029
Size: 365 231 433
Fragments arranged by size:
433 365 231
BsaWI w'CCGG_w
Cuts at: 0 863 929 1029
Size: 863 66 100
Fragments arranged by size:
863 100 66
BseYI C'CCAG_C
Cuts at: 0 493 936 1029
Size: 493 443 93
Fragments arranged by size:
493 443 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1029
Size: 364 665
BsiHKAI G_wGCw'C
Cuts at: 0 194 401 548 659 1029
Size: 194 207 147 111 370
Fragments arranged by size:
370 207 194 147 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 375 1029
Size: 375 654
Bsp1286I G_dGCh'C
Cuts at: 0 109 194 247 401 548 591 659 1029
Size: 109 85 53 154 147 43 68 370
Fragments arranged by size:
370 154 147 109 85 68 53 43
BspEI T'CCGG_A
Cuts at: 0 863 929 1029
Size: 863 66 100
Fragments arranged by size:
863 100 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 787 1029
Size: 386 401 242
Fragments arranged by size:
401 386 242
BsrBI CCG'CTC
Cuts at: 0 102 1029
Size: 102 927
BssSI C'ACGA_G
Cuts at: 0 118 238 1029
Size: 118 120 791
Fragments arranged by size:
791 120 118
BstXI CCAn_nnnn'nTGG
Cuts at: 0 273 345 1029
Size: 273 72 684
Fragments arranged by size:
684 273 72
BstYI r'GATC_y
Cuts at: 0 47 668 806 860 944 1029
Size: 47 621 138 54 84 85
Fragments arranged by size:
621 138 85 84 54 47
Bsu36I CC'TnA_GG
Cuts at: 0 673 1029
Size: 673 356
BtgI C'CryG_G
Cuts at: 0 266 1029
Size: 266 763
BtsI GCAGTG_nn'
Cuts at: 0 194 1029
Size: 194 835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 423 458 1029
Size: 423 35 571
Fragments arranged by size:
571 423 35
DraIII CAC_nnn'GTG
Cuts at: 0 120 1029
Size: 120 909
EaeI y'GGCC_r
Cuts at: 0 263 1011 1029
Size: 263 748 18
Fragments arranged by size:
748 263 18
EarI CTCTTCn'nnn_
Cuts at: 0 664 763 1029
Size: 664 99 266
Fragments arranged by size:
664 266 99
EcoICRI GAG'CTC
Cuts at: 0 546 657 1029
Size: 546 111 372
Fragments arranged by size:
546 372 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 247 645 772 1029
Size: 31 216 398 127 257
Fragments arranged by size:
398 257 216 127 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 828 1029
Size: 828 201
EcoO109I rG'GnC_Cy
Cuts at: 0 147 165 357 405 521 588 962 1029
Size: 147 18 192 48 116 67 374 67
Fragments arranged by size:
374 192 147 116 67 67 48 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 915 1029
Size: 915 114
HaeII r_GCGC'y
Cuts at: 0 368 1029
Size: 368 661
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 700 732 1029
Size: 700 32 297
Fragments arranged by size:
700 297 32
KasI G'GCGC_C
Cuts at: 0 364 1029
Size: 364 665
KpnI G_GTAC'C
Cuts at: 0 627 1029
Size: 627 402
MscI TGG'CCA
Cuts at: 0 265 1013 1029
Size: 265 748 16
Fragments arranged by size:
748 265 16
NarI GG'CG_CC
Cuts at: 0 365 1029
Size: 365 664
NcoI C'CATG_G
Cuts at: 0 266 1029
Size: 266 763
NspI r_CATG'y
Cuts at: 0 504 1029
Size: 504 525
PasI CC'CwG_GG
Cuts at: 0 591 1029
Size: 591 438
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 1029
Size: 348 681
PpuMI rG'GwC_Cy
Cuts at: 0 357 405 521 1029
Size: 357 48 116 508
Fragments arranged by size:
508 357 116 48
PspOMI G'GGCC_C
Cuts at: 0 587 1029
Size: 587 442
SacI G_AGCT'C
Cuts at: 0 548 659 1029
Size: 548 111 370
Fragments arranged by size:
548 370 111
SanDI GG'GwC_CC
Cuts at: 0 521 1029
Size: 521 508
SapI GCTCTTCn'nnn_
Cuts at: 0 664 1029
Size: 664 365
SfoI GGC'GCC
Cuts at: 0 366 1029
Size: 366 663
SmlI C'TyrA_G
Cuts at: 0 127 409 1029
Size: 127 282 620
Fragments arranged by size:
620 282 127
StuI AGG'CCT
Cuts at: 0 790 1001 1029
Size: 790 211 28
Fragments arranged by size:
790 211 28
StyI C'CwwG_G
Cuts at: 0 266 360 1029
Size: 266 94 669
Fragments arranged by size:
669 266 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 1029
Size: 345 684
XmnI GAAnn'nnTTC
Cuts at: 0 772 1029
Size: 772 257
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BglI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BssSI BstXI BstYI Bsu36I
BtgI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI MscI
NarI NcoI NspI PasI PflMI PpuMI PspOMI SacI
SanDI SapI SfoI SmlI StuI StyI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BsaAI BsaBI BsaXI BseRI
BsiEI BsiWI BsmI BspHI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI DrdI
EagI EciI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI MfeI MluI MmeI MslI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI ZraI